本文選題：隔藥餅灸 + 功能性胃腸病��； 參考：《湖南中醫(yī)藥大學(xué)》2014年碩士論文

【摘要】：目的觀察隔藥餅灸對功能性胃腸病(肝郁脾虛型)大鼠Ghrelin及VIP的影響,探討隔藥餅灸治療功能性胃腸病的可能作用機(jī)制。 方法60只健康SD大鼠雌雄分籠適應(yīng)性飼養(yǎng)3天,隨機(jī)分為5組,每組12只：空白對照組(A組)、模型組(B組)、隔藥餅灸組(C組)、多潘立酮組(D組)和逍遙散組(E組),除A組正常飼養(yǎng)外,其余4組均采用復(fù)合病因造模法(慢性束縛應(yīng)激+飲食失節(jié)+過度疲勞+夾尾+搖晃)造模,連續(xù)21天后,再按被試因素施加方法分組處理：A組正常飼養(yǎng)；B組行捆綁束縛30mim,并灌服生理鹽水；C組行隔藥餅灸30min,并灌服等量生理鹽水；D組行捆綁束縛30min,并灌服多潘立酮；E組行捆綁束縛30min,灌服逍遙散；共14天。實驗結(jié)束,禁食24h,麻醉處死,腹主動脈采血檢測VIP含量,取胃竇和結(jié)腸組織檢測Ghrelin含量,數(shù)據(jù)分析。 結(jié)果1、一般狀態(tài)：造模前,各組大鼠皮毛光澤,活潑好動,精神狀態(tài)良好,糞便球形,干濕適中；造模后,模型大鼠毛發(fā)散亂、枯黃無光澤,靜臥少動,精神狀態(tài)疲憊且煩躁,大便多稀溏；經(jīng)過治療后,隔藥餅灸組、多潘立酮組和逍遙散組大鼠皮毛轉(zhuǎn)光澤,活動增多,精神狀態(tài)好轉(zhuǎn),大便由稀變干。2、體重和食量：造模后,大鼠的食量減少,食欲減退,體重增長減緩,有統(tǒng)計學(xué)意義(P0.05或P0.01)。3、礦場試驗：造模前,大鼠活動度較高,經(jīng)造模,活動度減少,有統(tǒng)計學(xué)意義(P0.05或P0.01)。4、血清VIP含量：模型組與空白組比較,大鼠血清中VIP含量升高(P0.05),隔藥餅灸組、多潘立酮組和逍遙散組與模型組比較,VIP的含量均降低(P0.05或P0.01)。5、胃竇和結(jié)腸組織中Ghrelin蛋白的表達(dá)：模型組與空白組比較,大鼠胃竇和結(jié)腸組織中Ghrelin蛋白的表達(dá)下降(P0.05或P0.01)；隔藥餅灸組、多潘立酮組和逍遙散組與模型組比較,Ghrelin蛋白的表達(dá)上升(P0.01)。 結(jié)論隔藥餅灸能降低SD大鼠血清VIP含量,增加組織中Ghrelin蛋白的表達(dá),這可能是隔藥餅灸治療功能性胃腸病的機(jī)制之一。
[Abstract]:Objective to observe the effect of herbal cake moxibustion on Ghrelin and VIP in rats with functional gastrointestinal disease (liver stagnation and spleen deficiency) and to explore the possible mechanism of drug separated cake moxibustion in the treatment of functional gastrointestinal disease. Methods 60 healthy Sprague-Dawley rats were fed with male and female cages for 3 days and were randomly divided into 5 groups. There were 12 rats in each group: control group (A group), model group (group B), moxibustion group (group C) and group D (group D) and Xiaoyao powder group (group E), except for group A (normal feeding). The other four groups were made by the method of compound etiology (chronic restraint stress diet, excessive fatigue and tail-shaking) for 21 days. Then according to the factors applied to the subjects, they were divided into two groups: control group A, normal feeding group B, group B were treated by binding and binding 30 min, group C were given normal saline for 30 min, group D was given the same amount of normal saline for 30 min, and group E was given domperidone. Bind and bind for 30 minutes, take Xiaoyao San; A total of 14 days. At the end of the experiment, fasting for 24 hours, anesthetized death, abdominal aorta blood samples to detect the content of VIP, gastric antrum and colon tissue to detect the content of Ghrelin, data analysis. Results 1. General state: before modeling, the rats in each group had luster, liveliness, good mental state, globular feces, moderate dryness and wetness. After making the model, the hair of the model rats was scattered, yellow and luster was dull, the rats were still lying with little movement, and the mental state was tired and irritable. After treatment, the rats in the medicated cake moxibustion group, domperidone group and Xiaoyao powder group changed their fur to luster, their activities increased, their mental state improved, their defecation changed from dilute to dry, and their body weight and food intake decreased after making the model. Loss of appetite and slow growth of body weight, with statistical significance (P0.05 or P0.01P0.01U. 3). Field test: before modeling, the activity of rats was higher, and the activity of rats decreased after modeling. There was significant difference between model group and blank group (P 0.05 or P 0.01). The content of serum VIP in model group was higher than that in blank group, and there was no significant difference between the model group and the blank group. Compared with model group, the content of VIP in serum of rats increased P0.05A, the content of Ghrelin in moxibustion group, domperidone group and Xiaoyao powder group decreased P 0.05 or P0.01A. 5. The expression of Ghrelin protein in gastric antrum and colon: compared with blank group, the expression of Ghrelin protein in gastric antrum and colon: the model group was compared with the blank group. The expression of Ghrelin protein in gastric antrum and colon decreased by P0.05 or P0.01, and the expression of ghrelin protein increased in moxibustion group, domperidone group and Xiaoyao powder group compared with model group. Conclusion moxibustion with separated medicine cake can decrease the content of serum VIP and increase the expression of Ghrelin protein in the tissues of SD rats, which may be one of the mechanisms of treating functional gastrointestinal diseases by moxibustion with separated medicine cake.
【學(xué)位授予單位】：湖南中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R245

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