本文選題：當(dāng)歸揮發(fā)油 + 大鼠�。� 參考：《甘肅中醫(yī)學(xué)院》2014年碩士論文

【摘要】：目的： 本實驗旨在研究觀察當(dāng)歸揮發(fā)油對大鼠胃竇及十二指腸平滑肌肌電活動的影響，分析當(dāng)歸揮發(fā)油對胃腸平滑肌的作用特點及規(guī)律，為探討當(dāng)歸揮發(fā)油在防治消化道疾病方面的價值提供參考。 方法： Wistar大鼠禁食不禁水18h后，10%水合氯醛麻醉，手術(shù)暴露胃及小腸，分別用銀針電極自胃竇及十二指腸垂直于縱行肌斜刺入漿膜下2mm肌層，然后與BL-420F生物機(jī)能實驗系統(tǒng)相連接，建立胃腸平滑肌肌電實驗研究系統(tǒng)。設(shè)立阿托品組、維拉帕米組、多巴胺組為工具藥，以胃竇及十二指腸平滑肌慢波肌電的頻率和振幅變化等為觀察指標(biāo)，觀察當(dāng)歸揮發(fā)油對正常麻醉大鼠在體胃竇及十二指腸平滑肌慢波肌電活動、對新斯的明誘導(dǎo)的大鼠胃竇及十二指腸平滑肌慢波肌電活動、對氯化鋇誘導(dǎo)大鼠胃竇及十二指腸平滑肌慢波肌電活動及對嗎丁啉誘導(dǎo)的大鼠胃竇及十二指腸平滑肌慢波肌電活動的影響。 結(jié)果： 1）當(dāng)歸揮發(fā)油中劑量（0.2%）和低劑量（0.1%）下，麻醉大鼠胃竇平滑肌慢波肌電頻率和振幅呈現(xiàn)抑制效應(yīng)，而十二指腸平滑肌慢波肌電的頻率和振幅呈現(xiàn)興奮效應(yīng)；當(dāng)歸揮發(fā)油高劑量（0.4%）胃竇平滑肌慢波肌電的頻率和振幅呈現(xiàn)興奮效應(yīng)，，而十二指腸平滑肌慢波肌電的頻率和振幅則呈現(xiàn)抑制效應(yīng)。2）當(dāng)歸揮發(fā)油0.4%、0.2%、0.1%三個劑量組對新斯的明誘導(dǎo)的麻醉大鼠胃竇和十二指腸平滑肌慢波肌電頻率和振幅的變化均表現(xiàn)為明顯的拮抗作用，其作用曲線與拮抗藥阿托品的作用曲線相一致。3）當(dāng)歸揮發(fā)油0.2%、0.14%、0.1%三個劑量組對氯化鋇誘導(dǎo)的十二指腸平滑肌慢波肌電頻率的變化呈現(xiàn)明顯的拮抗作用，且效應(yīng)曲線特征與維拉帕米表現(xiàn)為高度的一致性。4）當(dāng)歸揮發(fā)油高劑量組對嗎丁啉誘導(dǎo)的麻醉大鼠胃竇和十二指腸平滑肌慢波肌電頻率的變化呈現(xiàn)明顯的拮抗作用，且效應(yīng)曲線特征與多巴胺表現(xiàn)為高度的一致性。 結(jié)論： 1）當(dāng)歸揮發(fā)油對麻醉大鼠胃竇和十二指腸平滑肌正常慢波肌電頻率和振幅具有相反的作用；2）不同濃度當(dāng)歸揮發(fā)油對麻醉大鼠胃竇和十二指腸平滑肌正常慢波肌電頻率和振幅具有“雙向”作用；3）當(dāng)歸揮發(fā)油對新斯的明誘導(dǎo)的麻醉大鼠胃竇和十二指腸平滑肌肌電慢波頻率和振幅的變化呈現(xiàn)明顯拮抗作用，該作用可能與阻滯M受體有關(guān)；4）當(dāng)歸揮發(fā)油對BaCl2誘導(dǎo)的麻醉大鼠十二指腸平滑肌慢波肌電頻率的變化呈現(xiàn)明顯的拮抗作用，該作用可能與阻滯鈣離子通道有關(guān)。5）當(dāng)歸揮發(fā)油對嗎丁啉誘導(dǎo)的麻醉大鼠十二指腸平滑肌慢波肌電頻率的變化呈現(xiàn)明顯的拮抗作用，該作用可能與興奮多巴胺受體有關(guān)。
[Abstract]:Objective: The purpose of this study was to observe the effect of volatile oil of Angelica sinensis on the myoelectric activity of gastric antrum and duodenal smooth muscle in rats, and to analyze the characteristics and regularity of the effect of volatile oil of Angelica sinensis on gastrointestinal smooth muscle. To explore the value of essential oil of Angelica sinensis in the prevention and treatment of digestive tract diseases. Methods: After fasting for 18 hours, Wistar rats were anesthetized with 10% chloral hydrate. The stomach and small intestine were exposed. Silver needle electrodes perpendicular to the longitudinal muscle from antrum and duodenum were applied to the subserous 2mm muscle layer, and then connected with the BL-420F biological function experimental system. To establish an experimental study system of gastrointestinal smooth muscle electromyography. Atropine group, verapamil group and dopamine group were used as tool drugs. The changes of frequency and amplitude of slow wave myoelectric activity in gastric antrum and duodenal smooth muscle were observed. To observe the slow wave myoelectric activity of gastric antrum and duodenal smooth muscle in normal anesthetized rats, and to observe the slow wave myoelectric activity of gastric antrum and duodenal smooth muscle induced by neostigmine. The effects of barium chloride on the slow wave myoelectric activity of gastric antrum and duodenal smooth muscle and morpholine induced slow wave myoelectric activity of gastric antrum and duodenal smooth muscle of rats were studied. Results: 1) the frequency and amplitude of slow wave myoelectricity in antral smooth muscle of anesthetized rats showed inhibitory effect, but the frequency and amplitude of slow wave electromyography in duodenal smooth muscle showed excitatory effect. The frequency and amplitude of slow wave electromyography of gastric antral smooth muscle showed excitatory effect. The frequency and amplitude of slow wave myoelectric activity in duodenal smooth muscle showed inhibitory effect. 2) the changes of frequency and amplitude of slow wave myoelectric activity induced by neostigmine in antral and duodenal smooth muscle of anesthetized rats treated with 0. 4% volatile oil of Angelica sinensis and 0. 1% 0. 1% of Angelica sinensis All of them showed obvious antagonistic effects. The action curve was consistent with that of antagonist atropine. 3) the volatile oil of Angelica sinensis 0.20.14and 0.1% had obvious antagonistic effect on the changes of slow wave frequency of duodenal smooth muscle induced by barium chloride. The characteristics of the effect curve were highly consistent with verapamil. 4) the high dose group of Angelica sinensis volatile oil showed obvious antagonistic effect on the changes of slow wave electromyography frequency of gastric antrum and duodenum smooth muscle induced by morpholine in anesthetized rats. The characteristics of the response curve were highly consistent with that of dopamine. Conclusion: 1) the volatile oil of Angelica sinensis has the opposite effect on the frequency and amplitude of normal slow wave myography in antrum and duodenal smooth muscle of anesthetized rats. (2) different concentrations of volatile oil of Angelica sinensis on normal slow wave myoelectric activity of gastric antrum and duodenal smooth muscle of anesthetized rats The frequency and amplitude have a "bidirectional" effect. The volatile oil of Angelica sinensis has a significant antagonistic effect on the changes of frequency and amplitude of myoelectric slow wave of gastric antrum and duodenal smooth muscle induced by neostigmine in anesthetized rats. This action may be related to blocking M receptor. The volatile oil of Angelica sinensis has an obvious antagonistic effect on the frequency of slow wave electromyography of duodenal smooth muscle induced by BaCl2 in anesthetized rats. This action may be related to blocking calcium channel. 5) Angelica volatile oil has obvious antagonistic effect on the changes of slow wave electromyography frequency of duodenal smooth muscle induced by morpholine in anesthetized rats, which may be related to excitatory dopamine receptor.
【學(xué)位授予單位】：甘肅中醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R285.5;R259

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