本文選題：特發(fā)性炎癥肌病 + 高遷移率族蛋白B1　； 參考：《遼寧中醫(yī)藥大學》2016年碩士論文

【摘要】：目的:研究單味中藥天花粉影響實驗性自身免疫性肌炎的大鼠血清肌酶(肌酸激酶、乳酸脫氫酶、天門冬氨酸氨基轉(zhuǎn)移酶)和HMGB1、TLR2、TLR4信號傳導改變的情況,進一步了解實驗性自身免疫性肌炎大鼠經(jīng)過天花粉干預后,肌肉炎癥損傷下的修復情況以及產(chǎn)生效果的機制。材料與方法:1.大鼠組別分配與免疫把四十只SPF級SD大鼠預先在實驗室中喂養(yǎng)一星期之后,仿效完全隨機設計的方法,把預先喂養(yǎng)的四十只SD大鼠隨機的分成四個組別,分別為:空白組、模型組、激素組、天花粉組。每組分別為十只。由異種動物(家兔)的骨骼肌作為肌勻漿的原料。制備好肌勻漿后將肌勻漿和完全弗氏佐劑各等份,把它們均勻攪拌后乳化。其中空白組中十只不用任何方法干預,剩下的三十只以之前調(diào)對好的等比例肌勻漿和完全弗氏佐劑進行免疫注射。注射的位置選取在大鼠背部的左右雙側(cè),實行皮下注射,每次注射量均為0.35毫升,每星期一次,此次注射后于第七天,第十四天,第二十一天,第二十八天,第三十五天對大鼠再進行注射,經(jīng)6次注射后,完成對大鼠的免疫。2.給藥觀察與提取樣本分析在免疫四個星期后,大鼠造模成功以后進行藥物干預�？瞻捉M和模型組采用生理鹽水來灌胃,而剩下兩組均采用每一組特有的處理方式進行灌胃。四組一共灌胃四周,四周后稱重大鼠的體重,分組記錄數(shù)據(jù),待體重測定完后麻醉,從腹主動脈中取血作為血液樣本,采集肌肉等相關組織作為肌肉樣本,后處死。3.處理樣本與分析樣本通過Lennon LA的評分細則,評定經(jīng)過藥物干預后大鼠的臨床狀態(tài),并加以評分;對最后一次灌胃后四組之間的體重進行對比;鑒定血清中肌酸激酶、乳酸脫氫酶、天門冬氨酸氨基轉(zhuǎn)移酶的值;通過酶聯(lián)免疫吸附法,獲得大鼠肌肉里HMGB1、TLR2、TLR4的值,并加以鑒定。結(jié)果:1.天花粉可以改善肌酸激酶(CK),乳酸脫氫酶(LDH),天門冬氨酸氨基轉(zhuǎn)移酶(AST)在血清中的含量,起到降低CK、LDH、AST的作用。2.天花粉對HMGB1、TLR4傳導產(chǎn)生影響,對TLR2傳導未產(chǎn)生影響。天花粉可減少HMGB1、TLR4在肌肉中的含量。結(jié)論:天花粉可以阻礙HMGB1、TLR4的傳導;降低血清肌酶在血液中的含量;但對TLR2傳導無影響。其可部分阻止免疫因子活化的程度,改善炎性細胞浸潤健康肌肉組織的程度,對于皮膚、肌肉等組織的炎癥導致皮膚肌肉損害起到了一定的抑制作用。天花粉對于治療特發(fā)性炎癥肌病具有一定作用。
[Abstract]:Objective: to study the effect of Trichosanthin on the signal transduction of serum creatine kinase, lactate dehydrogenase, aspartate aminotransferase and HMGB1 / TLR2 / TLR4 in rats with experimental autoimmune myositis. To investigate the repair of experimental autoimmune myositis and its mechanism after trichosanthin intervention. Materials and methods: 1. After 40 SPF SD rats were prefed in the laboratory for one week, forty prefed SD rats were randomly divided into four groups: blank group. Model group, hormone group, trichosanthin group. There were ten rats in each group. The skeletal muscle of xenogeneic animals (rabbits) is used as the raw material of muscle homogenate. The muscle homogenate and complete Freund's adjuvant were prepared and emulsified. Ten of the blank group did not need any intervention, and the remaining 30 were immunized with equal proportion muscle homogenate and complete Freund's adjuvant. The location of the injection was selected at the right and left sides of the back of the rat and subcutaneously injected. The dose of each injection was 0.35 ml, once a week. The injection was conducted on the seventh, fourteenth, 21, and 28 days after the injection. On the 35 day, the rats were injected again. After 6 times of injection, the rats were immunized. 2. 2. Drug administration observation and sample analysis were performed four weeks after immunization and after successful modeling in rats. The blank group and model group were perfused with normal saline, while the other two groups were treated by each group. The four groups were given gastric perfusion for four weeks. After four weeks, the weight of the rats was recorded in groups. After the weight was measured and anesthetized, blood was taken from the abdominal aorta as blood samples, muscle and other related tissues were taken as muscle samples, and then killed. The clinical status of the rats after drug intervention was evaluated and evaluated through the scoring rules of Lennon LA; the weight of the four groups after the last gastric perfusion was compared; and the serum creatine kinase was identified. Lactate dehydrogenase, aspartate aminotransferase and HMGB1TLR2TLR4 in rat muscle were obtained and identified by enzyme-linked immunosorbent assay (Elisa). The result is 1: 1. Trichosanthin can improve the contents of creatine kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST) in serum, and play a role in decreasing the activity of CKK LDHN AST. Trichosanth had an effect on HMGB1 TLR4 transmission, but had no effect on TLR2 transmission. Trichosanth can reduce the content of HMGB1 and TLR4 in muscle. Conclusion: Trichosanthin can block the transmission of HMGB1 and TLR4, decrease the content of serum myozyme in blood, but have no effect on TLR2 conduction. It can partly prevent the activation of immune factors, improve the degree of inflammatory cells infiltrating healthy muscle tissue, and inhibit the skin muscle damage caused by inflammation of skin, muscle and other tissues. Trichosanthin has a certain effect on the treatment of idiopathic inflammatory myopathy.
【學位授予單位】：遼寧中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R285.5
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本文編號：1803389