本文選題：低聲壓級(jí)水平次聲波 + 卵巢�。� 參考：《暨南大學(xué)》2014年碩士論文

【摘要】：研究目的 本研究通過觀察大鼠卵巢的結(jié)構(gòu)及測定雌二醇（E2）、睪酮（T）、卵泡刺激素（FSH）、黃體生成素（LH）的激素水平以探討低聲壓級(jí)水平次聲波（2～25Hz/70dB）短時(shí)間暴露對(duì)未孕SD大鼠卵巢功能的影響；通過測定雌三醇（E3）、孕酮（P）的激素水平及記錄仔鼠的數(shù)量、體質(zhì)量、身長、尾長、出生時(shí)的存活率、3天存活率、性別比等相關(guān)指標(biāo)以探討低聲壓級(jí)水平次聲波短時(shí)間暴露對(duì)孕鼠的胚胎和仔鼠的影響。 研究方法 將40只SD雌性大鼠和6只SD雄性大鼠飼養(yǎng)兩天適應(yīng)后隨機(jī)分組：實(shí)驗(yàn)1，對(duì)照組A1與低聲壓級(jí)水平次聲處理組A2各8只，低聲壓級(jí)水平次聲波處理方法為30分鐘/次×2次/天×20天，于第21天麻醉解剖兩組大鼠后取卵巢及血液并處理，卵巢組織經(jīng)HE染色后在光學(xué)顯微鏡下觀察其結(jié)構(gòu)并通過放射免疫分析法測定血清中的雌二醇（E2）、睪酮（T）、卵泡刺激素（FSH）、黃體生成素（LH）的激素水平；實(shí)驗(yàn)2，將24只雌鼠與6只雄鼠按4:1定時(shí)合籠，記錄每只孕鼠妊娠時(shí)間，并將18只孕鼠隨機(jī)分為對(duì)照組B1、妊娠期低聲壓級(jí)水平次聲處理組B2（30分鐘/次×2次/天×妊娠第1至20天）、妊娠中晚期低聲壓級(jí)水平次聲處理組B3（30分鐘/次×2次/天×妊娠第7至20天）各6只，，分別于妊娠第6、12、18天經(jīng)眼眶靜脈采血并處理，通過酶聯(lián)免疫法測血清中的雌三醇（E3）、孕酮（P）的激素水平，待孕鼠分娩后記錄仔鼠的數(shù)量、身長、尾長、體質(zhì)量、出生時(shí)存活率、3天存活率、性別比等相關(guān)指標(biāo)。 結(jié)果 實(shí)驗(yàn)1：對(duì)照組A1和低聲壓級(jí)水平次聲處理組A2大鼠血清中雌二醇（E2）、睪酮（T）、卵泡刺激素（FSH）、黃體生成素（LH）的激素水平的差異無統(tǒng)計(jì)學(xué)意義（P＞0.05）；與A1組相比，A2組卵泡的數(shù)量及形態(tài)無明顯改變，但髓質(zhì)層血管明顯擴(kuò)張。 實(shí)驗(yàn)2：對(duì)照組B1、妊娠期低聲壓級(jí)水平次聲處理組B2和妊娠中晚期低聲壓級(jí)水平次聲處理組B3在不同孕期大鼠血清中雌三醇（E3）、孕酮（P）的激素水平的差異均無統(tǒng)計(jì)學(xué)意義（P＞0.05）；B1、B2及B3組仔鼠的數(shù)量、身長、尾長、出生存活率、3天存活率、性別比無明顯差異（P＞0.05），與B1組相比， B2、B3組仔鼠的體質(zhì)量明顯增加（P＜0.05），B2與B3組仔鼠的體質(zhì)量無明顯差異（P＞0.05）。 結(jié)論 1.低聲壓級(jí)水平次聲短時(shí)間作用可以增加大鼠卵巢的血液供應(yīng)，對(duì)卵泡的發(fā)育、卵巢的內(nèi)分泌功能無明顯影響； 2.低聲壓級(jí)水平次聲短時(shí)間作用對(duì)孕鼠的激素水平無明顯影響，即對(duì)胚胎無明顯影響，故推測短時(shí)間的低聲壓級(jí)水平次聲用于孕婦是安全的； 3.低聲壓級(jí)水平次聲短時(shí)間作用可以增加仔鼠的體質(zhì)量，對(duì)仔鼠數(shù)量、身長、尾長、出生時(shí)存活率、3天存活率、性別比無明顯影響，故推測短時(shí)間的低聲壓級(jí)水平次聲波暴露對(duì)胎兒是安全的。
[Abstract]:Research purpose In this study, we observed the structure of rat ovary and measured the hormone levels of estradiol E _ (2), testosterone, follicle stimulating hormone (FSH) and luteinizing hormone (LH) in order to investigate the effects of infrasound at low sound pressure level on ovarian function in unpregnant SD rats after exposure to infrasonic waves of 25 Hz / 70 dB for a short time. By measuring the hormone levels of estriol E _ 3H, P) and recording the number, body weight, body length, tail length and 3-day survival rate at birth of young rats, Sex ratio and other related indexes were used to study the effects of infrasound exposure at low sound pressure level on embryo and offspring of pregnant rats. Research method Forty SD female rats and six SD male rats were fed for two days and were randomly divided into two groups: control group A1 and low sound pressure level infrasound group A 2, respectively. The treatment method of low sound pressure level infrasonic wave was 30 minutes / time 脳 2 times / day 脳 20 days. The ovary and blood of the two groups were taken and processed after anaesthetized dissection on the 21st day. After HE staining, the structure of ovarian tissue was observed under optical microscope. The levels of estradiol E _ (2), T _ (T), FSH and LH in serum were determined by radioimmunoassay. In experiment 2, 24 female mice and 6 male rats were caged at 4:1 to record the pregnancy time of each pregnant mouse. Eighteen pregnant rats were randomly divided into control group B1, infrasound group with low sound pressure level during pregnancy, group B 2o 30 minutes / times 脳 2 times per day 脳 1 to 20 days of pregnancy, infrasound group with low sound pressure level in the middle and third trimester of pregnancy treated with infrasound group for 30 minutes / time 脳 2 times / d. Each of the 6 rats was on the 7th to 20th day of pregnancy, The blood samples were collected from orbital vein on the 18th day of gestation, and the serum levels of estriol E _ (3) and P _ (P) were measured by enzyme-linked immunosorbent assay (Elisa). The number, length, tail length and body weight of the newborn rats were recorded after delivery. The survival rate at birth was 3 days, sex ratio and other related indicators. Result Experiment 1: there was no significant difference in serum estradiol E _ 2, T _ 2, FSH, LH between the control group A1 and the infrasound group A _ 2 in low sound pressure level (P > 0.05), and the number of follicles in A _ 2 group was higher than that in A _ 2 group (P < 0.05). There was no obvious change in quantity and shape. But the medullary layer vessels dilated obviously. Experiment 2: control group B1, infrasound group B2 during pregnancy and infrasound group B 3 in middle and late pregnancy had no significant difference in the levels of estriol E _ 3 and P) in serum of rats with different pregnancy. The number of litter rats in group B _ 1, B _ 2 and B _ 3, P > 0.05, There was no significant difference in sex ratio (P > 0.05) between group B _ 2 and group B _ 1, but there was no significant difference in body weight between group B _ 2 and group B _ 3 (P < 0.05) and group B _ 3 (P > 0.05). Conclusion 1. Infrasound at low sound pressure level could increase the blood supply of rat ovary, but had no obvious effect on follicle development and endocrine function of ovary. 2. Low sound pressure level infrasound had no obvious effect on hormone level of pregnant rats, that is, it had no obvious effect on embryo, so it is safe to use infrasound of low sound pressure level in pregnant women for a short time. 3. The effect of infrasound at low sound pressure level for short time could increase the body mass of young rats, but had no significant effect on the number, length, tail length, survival rate at birth and 3 days survival rate, and the sex ratio was not significantly affected. Therefore, it is speculated that the infrasound exposure of low sound pressure level for a short time is safe for the fetus.
【學(xué)位授予單位】：暨南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R714

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