發(fā)布時(shí)間：2018-04-18 10:49

  本文選題：肺纖維化 + 人參平肺方�。� 參考：《南京中醫(yī)藥大學(xué)學(xué)報(bào)》2017年01期

【摘要】：目的在二氧化硅粉塵懸液致大鼠纖維化模型上觀察人參平肺方的作用并探討其可能機(jī)制。方法 SD大鼠麻醉后在氣管內(nèi)注入二氧化硅粉塵懸液以建立肺纖維化模型,然后隨機(jī)分為模型組、潑尼松組(5.0mg/kg)、人參平肺方3.0、6.0、12.0g/kg劑量組,同時(shí)另設(shè)空白對(duì)照組。連續(xù)給藥48d后取大鼠肺組織測(cè)定肺系數(shù),HE及Masson染色觀察肺泡炎癥及纖維化程度,測(cè)試盒檢測(cè)肺組織中羥脯氨酸(HYP)含量,ELISA法檢測(cè)肺泡灌洗液TGF-β1、NF-κB、PDGF、TNF-α及IFN-γ水平,免疫組織化學(xué)染色檢測(cè)肺組織TGF-β1、Samd2/3及ERK1/2蛋白的表達(dá)。結(jié)果人參平肺方能夠顯著降低肺纖維化大鼠的肺系數(shù)(P0.05),降低肺泡炎癥及纖維化程度,降低肺泡灌洗液TGF-β1、NF-κB、PDGF及TNF-α水平(P0.05),升高肺泡灌洗液IFN-γ水平(P0.05),降低肺組織TGF-β1、Samd2/3及ERK1/2的蛋白表達(dá)(P0.05)。結(jié)論人參平肺方對(duì)肺纖維化大鼠有治療作用,其機(jī)制可能與TGF-β/Smad/ERK信號(hào)通路有關(guān)。
[Abstract]:Objective to observe the effect of Ginseng Pingfei recipe on the rat model of fibrosis induced by silica dust suspension and to explore its possible mechanism.Methods the model of pulmonary fibrosis was established by injecting silica dust suspension into the trachea of SD rats after anesthesia. The rats were randomly divided into two groups: model group, prednisone group (5.0 mg / kg), ginsenpingfei group (3.0 ~ 6.0 ~ 12.0 g / kg) and blank control group.After 48 days of continuous administration, lung tissue was taken from rats to measure lung coefficient, HE and Masson staining to observe the degree of alveolar inflammation and fibrosis. The content of hydroxyproline in lung tissue and the levels of TGF- 尾 1, NF- 魏 -PDGF- 偽 and IFN- 緯 in alveolar lavage fluid were detected by Elisa and the test box was used to detect the level of TGF- 尾 _ 1NF- 魏 -PDGF- 偽 and IFN- 緯 in alveolar lavage fluid.Immunohistochemical staining was used to detect the expression of TGF- 尾 1, Samd 2 / 3 and ERK1/2 protein in lung tissue.Results Ginseng Pingfei recipe could significantly decrease the lung coefficient of rats with pulmonary fibrosis (P0.05), reduce the degree of alveolar inflammation and fibrosis, decrease the levels of TGF- 尾 1, NF- 魏 B, PDGF and TNF- 偽 in alveolar lavage fluid, increase the level of IFN- 緯 in alveolar lavage fluid, and decrease the expression of TGF- 尾 _ 1 Samd _ 2 / 3 and ERK1/2 protein in lung tissue.Conclusion Ginseng Pingfei recipe has therapeutic effect on rats with pulmonary fibrosis, and its mechanism may be related to TGF- 尾 / Smad-ERK signaling pathway.
【作者單位】： 南京中醫(yī)藥大學(xué)藥學(xué)院;江蘇省中藥藥效與安全性評(píng)價(jià)重點(diǎn)實(shí)驗(yàn)室;南京中醫(yī)藥大學(xué)基礎(chǔ)醫(yī)學(xué)院;
【基金】：江蘇省中藥資源產(chǎn)業(yè)化過程協(xié)同創(chuàng)新中心重點(diǎn)課題(ZDXM-4) 江蘇省優(yōu)勢(shì)學(xué)科建設(shè)項(xiàng)目(PAPD) 國家自然科學(xué)基金(81473580)
【分類號(hào)】：R285.5

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