本文選題：飲食誘導肥胖 + 瘦素��； 參考：《天津醫(yī)科大學》2014年碩士論文

【摘要】：目的 通過建立飲食誘導肥胖(DIO)大鼠模型,觀察肥胖模型大鼠的血漿瘦素等生化指標的變化,測量血壓并進行血流動力學研究,觀察血壓改變與內皮功能的關系,探討瘦素對肥胖大鼠血壓影響的機制。 方法 1.選用SD雄性大鼠40只完全隨機分組,對照組(A組)10只喂以普通飼料,肥胖組30只喂以高脂飼料,根據(jù)大鼠體重及肥胖評定指數(shù)(Lee's指數(shù))判定肥胖模型是否成功。造模成功后隨機分為B、C、D三組各10只并且C、D兩組再分別高脂飼養(yǎng)2周及4周。 2.血清學檢測：造模成功后,分別于10周末、12周末及14周末大鼠麻醉動脈采血作血液生化檢查,采用酶法測定甘油三酯(TG)、膽固醇(TC)、葡萄糖(GLU)、高密度脂蛋白(HDL-C)水平。應用酶法測定一氧化氮(NO)、總一氧化氮合酶(TNOS)、誘導型一氧化氮合酶(iNOS)、內皮型一氧化氮合酶(eNOS).超氧陰離子、超氧化物歧化酶(SOD)水平,應用酶聯(lián)免疫吸附法檢測瘦素的水平。 3.血壓測定：不同組大鼠觀察期末將大鼠麻醉后分離暴露左頸靜脈及右頸動脈,然后經(jīng)左頸靜脈分別勻速推注乙酰膽堿和硝普鈉各5分鐘,推注速度為50μg/kg·min,同時經(jīng)右頸動脈插管,EPS電生理儀描記收縮壓演變過程。 結果 1.與對照組相比,飼養(yǎng)10周后肥胖組大鼠的體重較對照組顯著增加(P0.01), Lee's指數(shù)明顯增加(P0.05),以體重比正常組平均體重增加10%及Lee's指數(shù)比正常組平均水平增加1.5%作為判斷肥胖大鼠的標準,提示造模成功。 2.飲食誘導肥胖大鼠隨著飼養(yǎng)周期的增加,瘦素水平較對照組顯著增加(P0.01)。 3.與對照組對比,肥胖組隨著飼養(yǎng)周期的延長,NO、NOS及SOD血清水平均降低(P0.05), TNOS及iNOS及均升高(P0.05),而超氧陰離子水平?jīng)]有明顯變化(P0.05)。 4.頸動脈插管直接測血壓顯示：肥胖各組大鼠基礎收縮壓明顯高于對照組(P0.05),頸靜脈推注乙酰膽堿和硝普鈉,各組大鼠血壓水平明顯下降,對照組推注乙酰膽堿(P0.01)及硝普鈉(P0.05)引起降壓幅度高于肥胖組,頸靜脈推注乙酰膽堿和硝普鈉比較顯示,對照組間降壓幅度無統(tǒng)計學意義(P0.05),各個肥胖組間,硝普鈉降壓幅度高于乙酰膽堿P0.05),對照組間降壓幅度無統(tǒng)計學意義(P0.05)。
[Abstract]:PurposeThe changes of plasma leptin and other biochemical indexes were observed, blood pressure was measured and hemodynamics was studied, and the relationship between changes of blood pressure and endothelial function was observed by establishing diet-induced adipose-induced diol (Dio) rat model.To investigate the mechanism of leptin on blood pressure in obese rats.Method1.Forty Sprague-Dawley male rats were randomly divided into control group (n = 10) and obese group (n = 30).The rats were randomly divided into three groups (n = 10 each) and fed with hyperlipidemia for 2 weeks and 4 weeks respectively.2.Serological examination: after successful modeling, blood samples were collected from anesthetized arteries of rats at the end of 10 weeks, 12 weeks and 14 weeks, respectively. The levels of triglyceride triglyceride (TGN), cholesterol triglyceride (TCN), glucose glutin (GLU) and high density lipoprotein (HDL-C) were determined by enzymatic method.Total nitric oxide synthase (TNOS), inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (Enos) were determined by enzyme method.The levels of superoxide anion and superoxide dismutase (SOD) were determined by enzyme linked immunosorbent assay (Elisa).3.Blood pressure measurement: the left jugular vein and right carotid artery were isolated and exposed after anaesthesia in different groups of rats at the end of the observation period. Acetylcholine and sodium nitroprusside were injected through the left jugular vein for 5 minutes, respectively.The injection rate was 50 渭 g/kg, and the evolution of systolic blood pressure was recorded by right carotid artery intubation with EPS electrophysiological instrument.Result1.Compared with the control group,After 10 weeks of feeding, the body weight of obese rats was significantly higher than that of the control group, and the Lee's index was significantly higher than that of the control group. The average body weight of the obese rats was increased by 10% compared with the normal group and the Lee's index was 1.5% higher than the average level of the normal group.The results suggest that the model is successful.2.The level of leptin in obese rats induced by diet was significantly higher than that in the control group with the increase of feeding cycle.3.Compared with the control group, the serum levels of nitric oxide synthase (NOS) and SOD in obese group were decreased with the increase of feeding period, while the levels of TNOS and iNOS were increased, while the level of superoxide anion did not change significantly.4.Direct blood pressure measurement by carotid artery intubation showed that the basic systolic blood pressure of obese rats was significantly higher than that of the control group (P 0.05). The blood pressure of rats in each group was significantly decreased by injecting acetylcholine and sodium nitroprusside into the jugular vein.The hypotension amplitude of the control group was higher than that of the obese group. The comparison between the jugular vein injection of acetylcholine and sodium nitroprusside showed that there was no significant difference between the control group and the control group (P 0.05).Sodium nitroprusside was higher than acetylcholine P0. 05% in lowering blood pressure, but there was no significant difference between control group (P 0. 05 and P 0. 05).
【學位授予單位】：天津醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R589.2

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