本文選題：基因芯片技術(shù) + 胸腔積液��； 參考：《成都中醫(yī)藥大學(xué)》2014年博士論文

【摘要】：目的：利用基因芯片技術(shù),分析溫陽消飲法對胸腔積液大鼠胸膜基因表達(dá)的調(diào)控作用。 方法：48只雄性SD大鼠適應(yīng)性飼養(yǎng)3天后,隨機(jī)分為A組(40只)與空白對照組(8只)。A組動物以戊巴比妥鈉麻醉后,采用1%λ-角叉菜膠溶液進(jìn)行右側(cè)胸膜腔注射。24h后,運用CR數(shù)字成像系統(tǒng)觀察胸部X光片,篩選出胸腔積液明顯的大鼠,作為造模成功的動物備用。將造模成功的動物隨機(jī)分為：溫陽消飲組(10只),每日1次溫陽消飲法代表方水煎液灌胃(5ml/kg);模型組(9只),每日1次生理鹽水灌胃(5ml/kg)�？瞻讓φ战M(8只),每日1次生理鹽水灌胃(5ml/kg)。連續(xù)給藥4天后,動物進(jìn)行安樂死。每組取3只動物的右側(cè)臟層和壁層胸膜,利用Agilent單通道表達(dá)譜芯片對胸膜組織基因表達(dá)情況進(jìn)行分析。 結(jié)果： (1)壁層胸膜組織 模型組與空白對照組、溫陽消飲組與模型組差異表達(dá)基因涉及多個生物學(xué)過程和信號通路。在造模因素作用下,基因Fgd4、Mef2a、Fhl3、Dyrk2、Slc24a6、 Pbrm1、LOC498368、Tp53i11、Rcan3、Hapln4、Abil和Hlx表達(dá)上調(diào),在中藥干預(yù)下表達(dá)下調(diào)；Nme7、Pold2、Klk1l和Fkbp5在造模條件下表達(dá)下調(diào),用藥后上調(diào)。 (2)臟層胸膜組織 模型組與空白對照組、溫陽消飲組與模型組差異表達(dá)基因涉及多個生物學(xué)過程和信號通路。在造模因素作用下,基因Rhoa、RGD1306349、Nsd1、Figf、Brd4、 Crebbp、Sos2、Irf2、Brd2、Tspyl2、Eif4g2、Lcor、Isy1、Sf3a3、Msl2l1、Myo9a、 Papolg、Vasp、Pdcd7、Zbtb11、RGD1559904、Csnklg1、Leng8、Ddx10、Arhgef3、 Smg6、Zfat、Tm9sf3、Otud5和Chd6表達(dá)上調(diào),在溫陽消飲法代表方干預(yù)下表達(dá)下調(diào)；Ksr1、Slc25a27、Ptrf、Efemp1、RGD1563319、Cyb5r3、Gpr135、Polr3gl、 Kcna5、Ugp2、Acad11和Btbdll在造模條件下表達(dá)下調(diào),用溫陽消飲法代表方治療后上調(diào)。 結(jié)論：前期研究發(fā)現(xiàn),溫陽消飲法有助于λ-角叉菜膠誘導(dǎo)的豚鼠胸腔積液的吸收。本實驗表明,其機(jī)制可能與苓桂術(shù)甘湯合葶藶大棗瀉肺湯給藥調(diào)控多個生物學(xué)過程及信號通路有關(guān)。本研究從基因水平揭示了溫陽消飲法的部分機(jī)制：①溫陽消飲法可下調(diào)壁層胸膜炎癥促進(jìn)基因Mef2a、凋亡誘導(dǎo)基因Dyrk2和Tp53i11表達(dá),改善炎癥效應(yīng)。②溫陽消飲法可下調(diào)臟層胸膜炎癥促進(jìn)基因Rhoa、Figf、 Brd4、Crebbp和Brd2表達(dá),上調(diào)炎癥抑制基因Ksr1和Slc25a127表達(dá),從而降低炎性損害。此外,Fgd4、Fh13和Nme7等基因的表達(dá)變化與本研究的關(guān)系有待進(jìn)一步確認(rèn)。
[Abstract]:Aim: to analyze the effect of Wenyang Xiaoyin method on pleural gene expression in pleural effusion rats.Methods Forty eight male Sprague-Dawley rats were randomly divided into group A (n = 40) and control group (n = 8) after anesthesia with pentobarbital sodium, 1% 位 -carrageenin solution was injected into the right pleural cavity for 24 hours.The chest X-ray films were observed by CR digital imaging system and the rats with obvious pleural effusion were screened out.The successful animals were randomly divided into Wenyang Xiaoyin group (n = 10), Wenyang Xiaoyin group (n = 10) and normal saline group (n = 9).The control group (n = 8) was perfused with normal saline once a day (5 ml / kg).After 4 days of continuous administration, animals were euthanized.The right visceral and parietal pleura of 3 animals in each group were collected and the expression of genes in pleural tissue was analyzed by Agilent single channel microarray.Results:Parietal pleural tissueThe differentially expressed genes in model group and blank control group, Wenyang Xiaoyin group and model group were involved in many biological processes and signaling pathways.Under the action of modeling factors, the expression of Fgd4Mef2aFhl3Dyrk2C24a6, Pbrm1, LOC498368, Tp53i11Rcan3Rcan3Rcan3Hpln4Abil and Hlx were up-regulated, and the expression of Nme7Pold2Klk1l and Fkbp5 were down-regulated under the condition of modeling, and up-regulated after administration of traditional Chinese medicine.Visceral pleural tissueThe differentially expressed genes in model group and blank control group, Wenyang Xiaoyin group and model group were involved in many biological processes and signaling pathways.鍦ㄩ，

本文編號：1756155