本文選題：麻醉藥機制　切入點：七氟烷　出處：《四川大學(xué)學(xué)報(醫(yī)學(xué)版)》2016年05期

【摘要】：目的建立選擇性麻醉脊髓和腦的動物模型,探索七氟烷松弛骨骼肌作用的位點是否在脊髓。方法選擇健康新西蘭大白兔16只,通過體外循環(huán)技術(shù),于第12胸椎(T12)~第1腰椎(L1)水平結(jié)扎主動脈,從而形成上半身及下半身兩個相對獨立的循環(huán)系統(tǒng)。通過七氟烷經(jīng)肺或者氧合器給予到新西蘭大白兔上半身或者下半身,實現(xiàn)選擇性腦麻醉或者選擇性脊髓麻醉(主要是腰骶段脊髓)。連續(xù)監(jiān)測呼氣末(代表腦組織)和氧合器出氣口(代表脊髓)的七氟烷濃度。利用氣相色譜儀,注射器兩次平衡法來測量頸動脈(代表腦組織)和腹主動脈(代表脊髓)血液中七氟烷的質(zhì)量濃度和分壓。結(jié)果經(jīng)肺吸入1.5倍肺泡氣最低有效濃度(MAC)七氟烷并達平衡狀態(tài)時,呼氣末七氟烷濃度高于氧合器出氣口,頸動脈血液中七氟烷的質(zhì)量濃度和分壓高于腹主動脈(P0.05),即腦組織中的七氟烷濃度和分壓高于脊髓。經(jīng)過10~20min洗脫期后,由氧合器給予新西蘭大白兔下半身1.5 MAC七氟烷并達平衡狀態(tài)時,上述指標的表現(xiàn)則完全相反(P0.05),即脊髓中的七氟烷濃度和分壓高于腦組織。結(jié)論通過七氟烷的不同給予方式(經(jīng)肺或者氧合器),成功建立了七氟烷選擇性麻醉新西蘭大白兔腦、脊髓模型。
[Abstract]:Objective to establish an animal model of selective anesthesia of spinal cord and brain and to explore whether the site of sevoflurane relaxation of skeletal muscle is in spinal cord. Methods Sixteen healthy New Zealand white rabbits were selected by cardiopulmonary bypass (CPB). The aorta was ligated horizontally at the 12th thoracic vertebrae T12 ~ 1 L1) to form two relatively independent circulatory systems, the upper and lower half of the body, which were given to the upper or lower half of the New Zealand white rabbit via sevoflurane through the lung or oxygenator. Selective cerebral anesthesia or selective spinal anesthesia (mainly lumbosacral spinal cord). Continuous monitoring of sevoflurane concentrations at end expiratory (representing brain tissue) and oxygenator outlet (on behalf of spinal cord). Gas chromatograph, The mass concentration and partial pressure of sevoflurane in the blood of carotid artery (representing brain tissue) and abdominal aorta (representing spinal cord) were measured by the twice balance method of syringe. Results when inhaling 1.5 times the lowest effective concentration of alveolar gas through the lung and reaching the equilibrium state of sevoflurane, The concentration of sevoflurane at the end of expiratory period was higher than that in the outlet of oxygenator, and the mass concentration and partial pressure of sevoflurane in carotid blood were higher than that in abdominal aorta, that is, the concentration and partial pressure of sevoflurane in brain tissue were higher than those in spinal cord. When a New Zealand white rabbit was given 1.5 MAC sevoflurane in its lower body by oxygenator, On the contrary, the sevoflurane concentration and partial pressure in spinal cord were higher than that in brain tissue. Conclusion the sevoflurane selective anesthesia of the brain of New Zealand white rabbits was successfully established by different ways of giving sevoflurane (via lung or oxygenator). Spinal cord model.
【作者單位】： 四川大學(xué)華西醫(yī)院麻醉科;四川大學(xué)華西第二醫(yī)院麻醉科;四川大學(xué)華西醫(yī)院疼痛科;
【基金】：國家自然科學(xué)基金(No.81200865)資助
【分類號】：R614

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