發(fā)布時(shí)間：2018-03-02 18:24

  本文選題：咪達(dá)唑侖　切入點(diǎn)：大鼠神經(jīng)元　出處：《黑龍江畜牧獸醫(yī)》2017年05期 　論文類型：期刊論文

【摘要】：為了確定咪達(dá)唑侖對中樞神經(jīng)神經(jīng)元細(xì)胞氨基酸類遞質(zhì)谷氨酸(Glu)及甘氨酸(Gly)含量的影響,試驗(yàn)以胎鼠為研究動物進(jìn)行神經(jīng)元的體外培養(yǎng),分別設(shè)置高、中、低(60,5,3.5μg/m L)3個(gè)藥物濃度,每個(gè)藥物濃度設(shè)3組平行試驗(yàn),然后采用ELISA法檢測樣品中Glu與Gly的含量,對比含量變化分析咪達(dá)唑侖對大鼠神經(jīng)元氨基酸類神經(jīng)遞質(zhì)的影響。結(jié)果表明:不同濃度咪達(dá)唑侖對Glu呈現(xiàn)先增強(qiáng)后抑制的作用,而對Gly的作用表現(xiàn)為先抑制后增強(qiáng)。說明不同濃度咪達(dá)唑侖在麻醉過程中抑制興奮類氨基酸神經(jīng)遞質(zhì),增強(qiáng)抑制類氨基酸神經(jīng)遞質(zhì)。
[Abstract]:In order to determine the effect of midazolam on the contents of amino acid transmitter glutamate Gluand glycine Glycine in central nervous neuron cells, fetal mice were used as experimental animals to culture neurons in vitro. The concentrations of midazolam and midazolam were set at high, middle and low concentrations of 605 渭 g / mL, 3.5 渭 g / mL, respectively. The concentration of Glu and Gly in each drug was determined by ELISA method. The effects of midazolam on amino acid neurotransmitters in rat neurons were analyzed. The results showed that midazolam at different concentrations increased Glu first and then inhibited it. The effect of midazolam on Gly was inhibited at first and then increased, indicating that midazolam at different concentrations inhibited the excitatory amino acid neurotransmitters and enhanced the inhibition of amino acid neurotransmitters.
【作者單位】： 東北農(nóng)業(yè)大學(xué)動物醫(yī)學(xué)學(xué)院臨床外科學(xué)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金項(xiàng)目(31572580;31372491)
【分類號】：R965

【相似文獻(xiàn)】

相關(guān)期刊論文 前7條

1 凌衛(wèi)明;;癲癇患者血漿氨基酸神經(jīng)遞質(zhì)的含量測定及臨床意義[J];臨床檢驗(yàn)雜志;2008年06期

2 李s，

本文編號：1557657