本文關(guān)鍵詞： 細(xì)顆粒物 氫氣 氣道炎癥 氧化應(yīng)激 肺功能　出處：《河北醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:探討PM2.5在大鼠哮喘形成中的作用以及吸入氫氣對(duì)上述過程的干預(yù)效果。方法:1將35只wister雌性大鼠隨機(jī)分配到5個(gè)組內(nèi),分別為空白對(duì)照組、哮喘模型組、哮喘模型+H2組、哮喘模型+PM 2.5組、哮喘模型+PM 2.5+H2組,采用卵清白蛋白、氫氧化鋁干粉劑腹腔注射致敏及激發(fā)建立哮喘模型,于造模開始同時(shí)給與哮喘模型+PM 2.5組、哮喘模型+PM 2.5+H2組大鼠開始霧霾染毒,每天4小時(shí),每周5天,暴露30天,吸入霧霾濃度控制于0.6mg/m3左右,同時(shí),哮喘模型+PM 2.5+H2組大鼠于霧霾暴露完畢后同哮喘+氫氣組哮喘大鼠一起給予氫氣吸入治療,暴露后吸入含66%H2的氫氧混合氣,每天氫氣治療2小時(shí),持續(xù)30天。2各組大鼠于霧霾暴露第30天結(jié)束時(shí),應(yīng)用3%戊巴比妥鈉腹腔注射麻醉,氣管切開,插管,應(yīng)用肺功能儀檢測(cè)各組大鼠在霧化吸入不同濃度乙酰甲膽堿后氣道總阻力的變化;檢測(cè)完氣道阻力后,股動(dòng)脈取血處死大鼠,血液室溫放置,凝固后離心收集血清;打開胸腔,結(jié)扎右肺主支氣管,取3mL PBS灌洗大鼠左肺,重復(fù)灌洗3次,離心后取上清,采用ELISA法檢測(cè)大鼠血清和支氣管肺泡灌洗液(BALF)中IL-13和OVA-sIgE水平;取大鼠右肺上葉肺組織于4%甲醛中固定48小時(shí)后,用于肺組織病理,于電子顯微鏡下觀察各組大鼠肺組織病理結(jié)果。3數(shù)據(jù)分析:采用SPSS 21.0統(tǒng)計(jì)軟件對(duì)所有數(shù)據(jù)進(jìn)行統(tǒng)計(jì)分析。對(duì)于計(jì)量資料數(shù)據(jù)用均數(shù)土標(biāo)準(zhǔn)差來表示。檢測(cè)數(shù)據(jù)正態(tài)性及正態(tài)方差齊的計(jì)量資料采用單因素方差分析(One-Way ANOVA),進(jìn)一步兩兩比較均采用SNK法;以P0.05為具有統(tǒng)計(jì)學(xué)意義。結(jié)果:1大鼠霧化吸入0.9%氯化鈉溶液后各組之間氣道阻力無(wú)差異(P0.05);當(dāng)吸入乙酰甲膽堿濃度為6.25mg/mL時(shí),哮喘模型+PM 2.5組大鼠氣道阻力明顯高于其余各組,有統(tǒng)計(jì)學(xué)意義(P0.05),哮喘模型組大鼠氣道阻力高于空白對(duì)照組但與哮喘模型+H2組、哮喘模型+PM 2.5+H2組之間無(wú)差異(P0.05);吸入乙酰甲膽堿濃度升至12.5mg/mL時(shí),除哮喘模型+H2組與哮喘模型+PM 2.5+H2組無(wú)差異外,其余各組織間有明顯差異。2各組血清及灌洗液中SIgE水平與空白組相比明顯升高,兩組之間對(duì)比有統(tǒng)計(jì)學(xué)差異(P0.05);同哮喘模型組大鼠相比,哮喘模型+H2組大鼠血清及肺泡灌洗液中SIg E水平明顯降低,兩組之間對(duì)比有統(tǒng)計(jì)學(xué)差異(P0.05);哮喘模型+PM 2.5同哮喘組大鼠相比較,哮喘模型+PM 2.5組大鼠炎癥水平明顯高于哮喘模型組大鼠,兩組之間存在統(tǒng)計(jì)學(xué)差異(P0.05)。3對(duì)照組大鼠支氣管管腔通暢,黏膜上皮完整一致。哮喘模型組大鼠支氣管管腔狹窄;哮喘模型+PM2.5組大鼠支氣管管腔狹窄程度較哮喘模型組明顯加重;氫氣治療組大鼠其支氣管管腔狹窄程度較哮喘模型組大鼠略有改善,但較空白對(duì)照組嚴(yán)重結(jié)論:霧霾可以加重大鼠氣道反應(yīng)性,可以增加氣道對(duì)乙酰甲膽堿的敏感性,在哮喘形成過程中起到重要作用;氫氣的抗炎作用,減輕實(shí)驗(yàn)大鼠氣道炎癥,一定程度上可以改善大鼠的氣道高反應(yīng)性。
[Abstract]:Objective: To investigate the PM2.5 formation in rat asthma and the effects of inhalation of hydrogen in the process of intervention. Methods: 1 35 Wister female rats were randomly divided into 5 groups, namely control group, asthma group, asthma model group +H2, model +PM 2.5 asthma group, asthma model +PM in group 2.5+H2, using egg albumin, aluminum hydroxide powder agent sensitized intraperitoneally and induced asthma model, from the start of the model at the same time give asthma model +PM 2.5 group, +PM 2.5+H2 group, asthma model rats began haze exposure, 4 hours a day, 5 days a week, 30 days of exposure, the concentration of haze control in about 0.6mg/m3, inhalation at the same time, asthma model of +PM rats in the 2.5+H2 group after exposure to haze with asthma + H2 group asthmatic rats given together with hydrogen gas inhalation treatment, after exposure to inhalation of hydrogen oxygen mixed gas containing 66%H2, every hydrogen treatment for 2 hours, for 30 days in.2 group The rats in the haze exposed for thirtieth days at the end of anesthesia, using 3% sodium pentobarbital intraperitoneal injection of tracheotomy, intubation, application of pulmonary function testing in rats inhaled different concentrations of methacholine airway resistance; airway resistance after test, blood from the femoral artery of rats, blood at room temperature serum was collected after solidification, centrifugal; open the chest, ligation of right main bronchus lung, 3mL PBS left lung lavage in rats, 3 repeated lavage after centrifugation, the supernatant, serum and bronchoalveolar lavage fluid of rats was detected by the method of ELISA (BALF) IL-13 and OVA-sIgE levels; and the right lung upper lobe lung in rats in 4% formaldehyde fixed after 48 hours for pulmonary pathology in the electron microscope.3 data analysis of lung tissue of rats pathological results: using SPSS 21 statistical software for statistical analysis of all data for the measurement data used. The number of soil are expressed as standard deviation. The detection data of normality and homogeneity of variance of the normal measurement data using single factor analysis of variance (One-Way ANOVA), a further 22 were compared by SNK method; the P0.05 was considered statistically significant. Results: no differences between the 1 rats inhaled 0.9% Sodium Chloride Solution group airway resistance (P0.05); when inhaled methacholine concentration is 6.25mg/mL, the airway resistance in asthma model group +PM 2.5 rats was significantly higher than that of other groups, with statistical significance (P0.05), airway resistance in asthma rats in model group were higher than those in blank control group and asthma model group +H2, the difference between the asthma model group 2.5+H2 +PM (P0.05); acetyl the concentration of methacholine inhalation to 12.5mg/mL, in addition to the asthma model in +H2 group and +PM 2.5+H2 group had no difference in asthma model, all groups were significantly different between.2 serum and lavage fluid SIgE levels compared with the control group in Ming Dynasty Between the two groups significantly increased, compared with statistical difference (P0.05); compared with the asthma group, asthma model group serum +H2 and SIg level of E rats by bronchoalveolar lavage fluid was significantly lower, compared between the two groups had significant difference (P0.05); asthma model +PM 2.5 rats compared with the asthmatic group, inflammation the level of asthma model of +PM rats in 2.5 groups were significantly higher than asthma model group rats, there were significant differences between the two groups (P0.05).3 control group rat bronchial lumen patency intact mucous membrane. The bronchial asthma model group rat luminal stenosis; the degree of asthma model rats in the +PM2.5 group of bronchial stenosis compared with the asthma model group was significantly increased hydrogen; rats in the treatment group and the degree of bronchial stenosis compared with the asthma model group rats were slightly improved, but compared with blank control group conclusion: severe haze can aggravate the airway responsiveness to methacholine airway, can increase Choline sensitivity plays an important role in the development of asthma. The anti-inflammatory effect of hydrogen can alleviate airway inflammation in rats, and can improve airway hyperresponsiveness to some extent.

【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R562.25

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