發(fā)布時間：2018-02-27 03:01

  本文關(guān)鍵詞： 四君子湯 慢性心衰 心肌蛋白質(zhì)組學 Suclg 絲氨酸蛋白酶抑制劑 丙酮酸脫氫酶 肌球蛋白輕鏈　出處：《中國實驗方劑學雜志》2017年05期 　論文類型：期刊論文

【摘要】：目的:探討四君子湯對慢性心衰大鼠心肌組織蛋白質(zhì)表達變化的影響。方法:SD雄性大鼠42只,隨機取6只作為正常組,其余36只復制慢性心力衰竭大鼠模型,造模成功后,分為四君子湯高、低劑量組(11.88,2.97 g·kg~(-1)·d~(-1)),卡托普利組(100 mg·kg~(-1)·d~(-1)),正常組和模型組分別給予相同體積生理鹽水,連續(xù)灌胃給藥1月。分別留取各組左心室組織,雙向凝膠電泳分離心肌蛋白,分析差異顯示的蛋白,并對有顯著差異的蛋白點進行膠內(nèi)酶切和質(zhì)譜鑒定。結(jié)果:與模型組比較,四君子湯組有31種蛋白質(zhì)表達上調(diào),28種蛋白質(zhì)表達下調(diào),各選取8個差異顯著(Volume值相差≥1.5倍)的蛋白進行質(zhì)譜鑒定,通過整合去重,鑒定出4個蛋白:與能量代謝相關(guān)的Suclg2蛋白、丙酮酸脫氫酶復合體;與心肌細胞收縮功能相關(guān)的肌球蛋白輕鏈2;與心肌細胞重構(gòu)相關(guān)的絲氨酸蛋白酶抑制劑A3N。結(jié)論:四君子湯可調(diào)節(jié)慢性心衰大鼠心肌組織多種蛋白表達,可能通過調(diào)節(jié)能量代謝、減少心肌重構(gòu)、提高收縮功能而改善心功能。
[Abstract]:Objective: to investigate the effect of Sijunzi decoction on the changes of myocardial protein expression in chronic heart failure rats. Methods: 42 male SD rats were randomly selected as normal group and the other 36 rats were duplicated with chronic heart failure model. The rats were divided into four groups: Sijunzi decoction was high, low dose group was 11.88g 路kg ~ (-1)) 路D ~ (-1), captopril group was 100 mg 路kg ~ (-1) 路d ~ (-1) ~ (-1), normal group and model group were given the same volume of normal saline respectively on January, left ventricular tissue was isolated by two-dimensional gel electrophoresis, myocardial protein was isolated by two-dimensional gel electrophoresis. Results: compared with the model group, 31 proteins were up-regulated and 28 proteins were down-regulated in Sijunzi decoction group. Eight proteins with significant difference (volume value 鈮，

本文編號：1540805