本文關(guān)鍵詞： 熒光探針 示蹤 間充質(zhì)干細(xì)胞 急性肺損傷 聚集　出處：《中山大學(xué)學(xué)報(醫(yī)學(xué)科學(xué)版)》2017年03期 　論文類型：期刊論文

【摘要】：【目的】通過構(gòu)建小鼠急性肺損傷(ALI)模型,示蹤間充質(zhì)干細(xì)胞(MSC)治療急性肺損傷�！痉椒ā恳訫SC表面神經(jīng)節(jié)苷脂(GD2)特異性抗原靶向的單克隆抗體Anti-GD2為載體,與熒光菁染料(Cy7)共價結(jié)合,得到新型熒光分子探針Anti-GD2-Cy7。將合成的熒光分子探針Anti-GD2-Cy7通過抗原抗體反應(yīng)標(biāo)記在MSC上。本研究取84只Balb/c雄性小鼠,隨機抽取其中48只平均分成三組:假手術(shù)組(n=16)、MSC+ALI組(n=16)和NS+ALI組(n=16)。建立ALI模型后,在24 h、48 h時間點進行肺組織形態(tài)學(xué)觀察和病理評分、檢測肺濕/干質(zhì)量(W/D)比值和肺微血管通透性改變。剩余36只Balb/c雄性小鼠隨機分為正常組(n=12)、假手術(shù)組(n=12)和MSC+ALI組(n=12),將標(biāo)記上的MSC-GD2-Cy7通過尾靜脈注射入假手術(shù)組和MSC+ALI組小鼠。分別在尾靜脈注射后的30 min及1 d、3 d、7 d各時間點麻醉解剖小鼠后;取其肺在小動物熒光成像儀下進行檢測�！窘Y(jié)果】模型制備達到ALI水平,無論是治療后的24 h還是48 h,MSC+ALI組在肺組織的病理及評分、肺組織的水腫程度和肺血管的通透性明顯比NS+ALI組輕。熒光檢測顯示MSC+ALI組在各時間點肺部平均熒光強度顯著高于假手術(shù)組[(3.37±0.02)×10~(-4)vs(2.05±0.04)×10~(-4)scaled counts/s;(35.54±0.47)×10~(-4)vs(25.29±1.48)×10~(-4)scaled counts/s;(11.17±0.75)×10~(-4)vs(6.09±0.62)×10~(-4)scaled counts/s;(3.10±0.14)vs(0.00±0.00)×10~(-4)scaled counts/s;且P均小于0.05)]�！窘Y(jié)論】間充質(zhì)干細(xì)胞在注射后的30 min即可遷移到正常肺和損傷肺中,且在第1天大量的聚集在損傷肺中并持續(xù)到第7天;這表明MSC具有在損傷部位聚集的作用。
[Abstract]:[objective] to establish a mouse model of acute lung injury (Ali) and to treat acute lung injury with the tracer of mesenchymal stem cells (MSCs). [methods] Anti-GD2, a monoclonal antibody targeting MSC surface ganglioside GD2, was used as vector. A novel fluorescent molecular probe, Anti-GD2-Cy7, was obtained by covalent binding with Cy7. The synthesized fluorescent probe Anti-GD2-Cy7 was labeled on MSC by antigen-antibody reaction. 84 male Balb/c mice were used in this study. 48 of them were randomly divided into three groups: sham operation group (ALI group) and NS ALI group (n = 16). Lung histomorphology and pathological score were observed at 24 h and 48 h after the establishment of ALI model. Lung wet / dry weight / W / D ratio and pulmonary microvascular permeability were measured. The remaining 36 male Balb/c mice were randomly divided into normal group (n = 12), sham-operated group (n = 12) and MSC ALI group (n = 12). The labeled MSC-GD2-Cy7 was injected via tail vein into sham operation group and MSC ALI group. The anaesthetized mice were anaesthetized at 30 min and 1 d 3 d after caudal vein injection respectively. [results] the model was made up to the level of ALI, both 24 h after treatment and 48 h after treatment. The average fluorescence intensity of lung tissue in the MSC ALI group was significantly higher than that in the sham operation group [3.37 鹵0.02) 脳 10 ~ (-4) VSD 2.05 鹵0.05 鹵0.04) 脳 10 ~ (10) -4scaled countsrsr (35.54 鹵0.47) 脳 10 ~ (-4) VS ~ (25.29 鹵1.48) 脳 10 ~ (-4) cases / r = 11.17 鹵0.75) 脳 10 ~ (10) ~ (-0. 75) 脳 10 ~ (-4) V ~ (-1) P = 6.09 鹵0.62) 脳 10 ~ (10) ~ (-0. 14) 鹵0. 10 鹵0. 14 鹵0. 14 鹵0. 14 鹵0. 00 鹵0. 14 鹵0. 05 鹵0. 14%. [P < 0. 05] [P < 0. 05] [P < 0. 05] [P < 0. 05] [P < 0. 05] [P < 0. 05]. The stem cells migrated to the normal and injured lungs 30 min after injection. On the first day, the MSC was accumulated in the injured lung and lasted until the 7th day, which indicated that MSC had the effect of aggregation at the injured site.
【作者單位】： 中山大學(xué)附屬第三醫(yī)院外科ICU;
【基金】：廣東省科技計劃項目(2014A020211010) 廣州市天河區(qū)科技計劃項目(2013kw028)
【分類號】：R563.8

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