本文關(guān)鍵詞： 高原訓練 高壓氧艙 自由基 大鼠　出處：《青海師范大學》2014年碩士論文　論文類型：學位論文

【摘要】：目的：通過測定大強度運動后進入不同時間、不同壓強下增壓氧艙恢復后的大鼠血清自由基接相關(guān)指標的含量，探討高原低氧環(huán)境下大強度運動后增壓輔助方法對大鼠恢復情況的影響，為進一步研究增壓輔助方法對運動員疲勞恢復提供研究基礎。方法：將56只Wistar大鼠隨機分成7組，（1）高原安靜組，安靜飼養(yǎng)（2）高原訓練組，大負荷訓練后不用任何方法幫其疲勞恢復（3）高原訓練后進入壓力為0.2MPa的增壓氧艙恢復一小時的增壓組（4）高原訓練后進入壓力為0.2MPa的增壓氧艙恢復兩小時的增壓組（5）高原訓練后進入壓力為0.3MPa的增壓氧艙恢復一小時的增壓組（6）模擬海拔4500m低氧訓練組，進入模擬海拔4500m大負荷訓練后不用任何方法幫其疲勞恢復（7）進入模擬海拔4500m進行低氧訓練后再進入壓力為0.2MPa的增壓氧艙恢復一小時的增壓組，正式訓練6天結(jié)束后第7天將所有大鼠分批進行跑臺至力竭，24小時休息后麻醉處死。測定各組大鼠血清中SOD、CAT、GSH-Px、T-AOC、MDA、ROS的濃度。結(jié)果：大鼠在海拔2261m的環(huán)境下，進行6天的低氧訓練后，進增壓氧艙壓力增加至0.2MPa一小時，會使大鼠血清中SOD濃度升高，MDA濃度降低，ROS濃度降低，CAT、GSH-Px、T-AOC濃度均無顯著性差異。大鼠在海拔2261m的環(huán)境下，，進行6天的低氧訓練后，進增壓氧艙壓力增加至0.2MPa兩小時，會使大鼠血清中SOD濃度升高，CAT濃度下降、MDA濃度降低，GSH-Px、T-AOC、ROS濃度均無顯著性差異。大鼠在海拔2261m的環(huán)境下，進行6天的低氧訓練后，進增壓氧艙壓力增加至0.3MPa一小時會使大鼠血清中CAT濃度下降，MDA濃度下降，SOD、GSH-Px、T-AOC、ROS濃度均無顯著性差異。大鼠在模擬海拔4500m的環(huán)境下，進行6天的低氧訓練后，進增壓氧艙壓力增加至0.2MPa一小時，會使大鼠血清中ROS濃度升高，SOD、CAT、GSH-Px、MDA、 T-AOC濃度均無顯著性差異。結(jié)論：大鼠經(jīng)海拔2261m環(huán)境下進行6天的低氧訓練后，分別進入壓力0.2MPa一小時或兩小時、0.3MPa一小時后均會使血清中自由基濃度下降，對疲勞恢復有積極的作用。大鼠經(jīng)海模擬海拔4500m環(huán)境下進行6天的低氧訓練后，進入壓力0.2MPa一小時后會使血清中自由基濃度上升，對疲勞恢復有負面作用。大鼠經(jīng)海拔2261m環(huán)境下進行6天的低氧訓練后，進增壓氧艙壓力增加到0.2MP，一小時或兩小時，效果最好，壓力加到0.3MP，一小時，效果次之。
[Abstract]:Objective: to determine the content of serum free radical grafting indexes in rats after high intensity exercise at different time and pressure after recovery of hyperbaric oxygen chamber. Objective: to investigate the effect of hyperbaric auxiliary method after high intensity exercise on the recovery of rats under high altitude hypoxia. Methods: 56 Wistar rats were randomly divided into 7 groups (n = 7). (2) High altitude training group. After heavy load training, no method is needed to help it recover from fatigue. After high altitude training, it enters the pressurized oxygen chamber with pressure of 0.2 MPA and recovers for one hour. After altitude training, enter the pressurized oxygen chamber with a pressure of 0.2MPa and resume the pressurized oxygen tank for two hours.) after the altitude training, enter the pressurized oxygen chamber with the pressure of 0.3MPa and recover the pressurized oxygen tank for one hour. Simulated altitude 4500m hypoxia training group. Enter the simulated altitude of 4500m heavy load training without any means to help its fatigue recovery. After hypoxic training at simulated altitude of 4500m, the pressurized oxygen chamber with a pressure of 0.2MPa returned to the pressurized group for one hour. On the 7th day after 6 days of formal training, all rats were anesthetized and killed after 24 hours' rest, and the serum GSH-PxX T-AOC of the rats in each group was determined. Results: after 6 days of hypoxic training, the pressure of oxygen chamber increased to 0.2 MPA for one hour. It can increase the concentration of SOD and decrease the concentration of Ros in serum of rats. There was no significant difference in T-AOC concentration. After 6 days of hypoxic training, the pressure of the pressurized chamber increased to 0.2 MPA for two hours at 2261m altitude. It can increase the concentration of SOD in serum of rats and decrease the concentration of cat. The concentration of malondialdehyde (MDA) decreases the concentration of GSH-PxX T-AOC. There was no significant difference in the concentration of ROS. After 6 days of hypoxic training, rats were exposed to hypoxia at an altitude of 2261m. When the pressure of oxygen chamber increased to 0.3 MPA for one hour, the concentration of CAT in serum of rats decreased and the concentration of MDA decreased. There was no significant difference in the concentration of ROS. After 6 days of hypoxic training, the pressure of the pressurized chamber increased to 0.2 MPA for one hour under the simulated altitude of 4500m. The concentration of ROS in serum of rats was increased. There was no significant difference in T-AOC concentration. Conclusion: after 6 days of hypoxic training at 2261m altitude, the rats entered the pressure of 0.2MPa for one hour or two hours, respectively. The concentration of free radicals in serum decreased after 0.3 MPA for one hour, which had a positive effect on the recovery of fatigue. After 6 days of hypoxia training at simulated altitude of 4500m, rats were trained at sea level for 6 days. The concentration of free radicals in serum increased after entering the pressure of 0.2MPa for one hour, which had a negative effect on fatigue recovery. The rats were trained at 2261m altitude for 6 days after hypoxic training. The pressure of oxygen chamber is increased to 0.2 MPP, one hour or two hours, the effect is the best, the pressure is 0.3 MPP, one hour, the effect is second.
【學位授予單位】：青海師范大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：G804.2

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