發(fā)布時(shí)間：2018-01-16 09:47

  本文關(guān)鍵詞：綠茶對(duì)肺癌的干預(yù)及其機(jī)制初探　出處：《南昌大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 肺癌 綠茶 化學(xué)預(yù)防 P53 Survivin VEGF

【摘要】：目的: 肺癌在當(dāng)今全球范圍之內(nèi)，已成為發(fā)病率增長(zhǎng)最為迅速，而預(yù)后最差的惡性腫瘤。由于在肺癌的早期診斷方面，仍未發(fā)現(xiàn)高靈敏度的特異性檢測(cè)技術(shù)和篩查方法，人們更迫切期待能通過(guò)日常飲食等手段，積極預(yù)防肺癌的發(fā)病�；瘜W(xué)預(yù)防是癌癥預(yù)防的關(guān)鍵要素。而更多的流行病學(xué)和實(shí)驗(yàn)研究證明，健康的飲食和生活方式等因素能明顯降低肺癌的患病風(fēng)險(xiǎn)。茶是全球飲用范圍最為普及的飲料之一。綠茶具有良好的防癌功效。人們期待，能通過(guò)飲用綠茶這種生活方式，有效的預(yù)防肺癌發(fā)病。與此同時(shí)，構(gòu)建肺部惡性腫瘤動(dòng)物模型是評(píng)估肺癌防治療效必要的實(shí)驗(yàn)方法。本課題應(yīng)用苯并芘，這種因吸煙及空氣污染而產(chǎn)生的，在人們?nèi)粘Ｉ钪薪佑|最為頻繁的高活性致癌物，經(jīng)肺部穿刺操作，對(duì)實(shí)驗(yàn)大鼠進(jìn)行注射，構(gòu)建大鼠肺部惡性腫瘤動(dòng)物模型，通過(guò)這一模型，達(dá)到評(píng)價(jià)綠茶對(duì)肺部惡性腫瘤的干預(yù)作用，并在此基礎(chǔ)之上，更深入的研究綠茶對(duì)大鼠肺部惡性腫瘤組織在p53蛋白和survivin蛋白表達(dá)上的影響。同時(shí)，通過(guò)酶聯(lián)免疫吸附試驗(yàn)，檢測(cè)大鼠動(dòng)物模型VEGF的表達(dá)，以此初步探明綠茶對(duì)肺部惡性腫瘤的干預(yù)機(jī)制。 方法: 構(gòu)建大鼠肺部惡性腫瘤模型。應(yīng)用3,4-苯并芘粉末混于玉米油溶液，配制成混合溶液，每次均固定注射含有3,4-苯并芘2mg劑量的玉米油混合溶液0.2ml。使用10%水合氯醛腹腔注射麻醉后，于大鼠右側(cè)3-4肋間經(jīng)皮肺穿刺注射。對(duì)照組則使用純玉米油溶液做肺部穿刺注射。各組均每隔2周操作1次，總共操作4次。 在綠茶干預(yù)肺部惡性腫瘤實(shí)驗(yàn)組中，，僅使用綠茶作為日常的唯一飲料進(jìn)行化學(xué)預(yù)防。而飲水組和對(duì)照組則使用飲用水作為日常的飲料。觀察大鼠肺部惡性腫瘤病變過(guò)程，通過(guò)免疫組織化學(xué)方法，檢測(cè)綠茶組和飲水組大鼠肺部腫瘤組織p53蛋白和survivin蛋白表達(dá)差異。同時(shí)，通過(guò)酶聯(lián)免疫吸附試驗(yàn)，檢測(cè)各組大鼠血清VEGF表達(dá)的差異。 結(jié)果: 所有單純接受玉米油溶液肺部穿刺注射的對(duì)照組大鼠，在52周實(shí)驗(yàn)周期內(nèi)未發(fā)現(xiàn)腫瘤。飲水組在52周內(nèi)惡性腫瘤發(fā)病率為65%，而綠茶組在52周內(nèi)惡性腫瘤發(fā)病率明顯下降，僅為20%。免疫組織化學(xué)結(jié)果顯示:綠茶組肺部惡性腫瘤組織p53蛋白表達(dá)上調(diào)、survivin蛋白表達(dá)下調(diào)，且與飲水組比較，在表達(dá)差異上具有統(tǒng)計(jì)學(xué)意義(P0.05)。酶聯(lián)免疫吸附試驗(yàn)檢測(cè)結(jié)果顯示:綠茶組VEGF表達(dá)下調(diào)，且與飲水組相比，在表達(dá)差異上具有統(tǒng)計(jì)學(xué)意義(P0.05)。 結(jié)論: 綠茶對(duì)3,4-苯并芘致癌物所致的肺部惡性腫瘤具有較為明顯的預(yù)防和干預(yù)作用。通過(guò)上調(diào)p53蛋白的表達(dá)，下調(diào)survivin蛋白和VEGF的表達(dá)是綠茶預(yù)防肺癌的重要機(jī)制。
[Abstract]:Objective: Lung cancer has become the most rapidly increasing incidence and the worst prognosis of malignant tumors in the world today, due to the early diagnosis of lung cancer. High sensitivity specific detection techniques and screening methods have not yet been found, and people are looking forward to daily diet and other means. Active prevention of lung cancer. Chemical prevention is a key element of cancer prevention. A healthy diet and lifestyle can significantly reduce the risk of lung cancer. Tea is one of the most popular drinks in the world. Green tea has a good anti-cancer effect. It can effectively prevent lung cancer by drinking green tea. At the same time, it is necessary to establish animal model of lung cancer to evaluate the curative effect of lung cancer. This highly active carcinogen, which is produced by smoking and air pollution, is the most frequently exposed carcinogen in people's daily life. It is injected into experimental rats through lung puncture to establish the animal model of lung malignant tumor in rats. Through this model, the intervention effect of green tea on lung malignant tumor was evaluated, and on this basis. To study the effect of green tea on the expression of p53 protein and survivin protein in lung malignant tumor tissues of rats. At the same time, enzyme linked immunosorbent assay (Elisa) was used. To investigate the mechanism of green tea intervention on lung malignant tumor, the expression of VEGF in rat model was detected. Methods: The model of lung malignant tumor in rats was established. The mixed solution was prepared by mixing 3o 4-benzo pyrene powder in corn oil solution and containing 3% in each injection. The dosage of 4-benzopyrene was 0.2 ml of corn oil mixed solution. 10% chloral hydrate was injected intraperitoneally after anesthesia. The right 3-4 intercostal lung puncture was performed on the right side of the rats, while the control group was injected with pure corn oil solution once every 2 weeks for a total of 4 times. In the experimental group of green tea intervention lung malignant tumor. Only green tea was used as the only daily drink for chemical prophylaxis, while drinking water was used as daily drink in drinking water group and control group. The pathological process of lung malignant tumor in rats was observed by immunohistochemical method. The expression of p53 protein and survivin protein in lung tumor tissue of green tea group and drinking group were detected. At the same time, enzyme linked immunosorbent assay (Elisa) was used. The difference of serum VEGF expression in each group was detected. Results: No tumor was found in all the control rats who received pure pulmonary puncture injection of corn oil solution. The incidence of malignant tumor in drinking water group was 65% within 52 weeks. However, the incidence of malignant tumor in green tea group decreased significantly within 52 weeks, only 20%. Immunohistochemical results showed that p53 protein expression was up-regulated in lung malignant tumor tissue of green tea group. The expression of survivin protein was down-regulated and compared with the drinking water group. The results of Elisa showed that the expression of VEGF in green tea group was down-regulated and compared with that in drinking water group. The difference of expression was statistically significant (P 0.05). Conclusion: Green tea has obvious preventive and interventional effects on lung malignant tumors caused by carcinogens of 3o 4-benzo pyrene. It can up-regulate the expression of p53 protein. Down-regulating the expression of survivin protein and VEGF is an important mechanism of preventing lung cancer in green tea.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R734.2

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