本文關(guān)鍵詞：術(shù)后腸梗阻對(duì)大鼠下丘腦和腦干nesfatin-1表達(dá)的影響　出處：《青島大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 腹部手術(shù) Fos 下丘腦 Nesfatin-1 術(shù)后腸梗阻 腦干

【摘要】：目的：觀察術(shù)后腸梗阻對(duì)大鼠腦內(nèi)nesfatin-1表達(dá)的影響。 方法：大鼠經(jīng)腹腔注射硫酸仲丁巴比妥(100～150mg/kg)麻醉后行腹部手術(shù),術(shù)后2小時(shí)后經(jīng)心灌注固定,免疫組化觀察大鼠腦內(nèi)c-Fos和nesfatin-1免疫陽性神經(jīng)元的表達(dá)。 結(jié)果：手術(shù)組大鼠下丘腦視上核(SON)(t=6.9,p0.01),室旁核(mpPVN(t=17.2; p0.01), mmPVN (t=25.8, p0.01),1mPVN (t=19.5; p0.01)和apPVN(t==22.1;p0.01)),藍(lán)斑(LC)(t=2.6,p0.05),動(dòng)眼神經(jīng)副核(EW)(t=10.74, p0.01),中縫蒼白核(rRPa)(t=2.57,p0.05),孤束核(NTS)(t=10.46,p0.01),延髓腹外側(cè)區(qū)(VLM)(t=7.86,p0.01)神經(jīng)元c-Fos表達(dá)顯著增加,但nesfatir-1免疫陽性神經(jīng)元表達(dá)無顯著改變(P0.05)。但統(tǒng)計(jì)分析發(fā)現(xiàn),腹部手術(shù)激活的c-Fos神經(jīng)元中,在下丘腦SON內(nèi)99%、apPVN內(nèi)71%、1mPVN內(nèi)47%、mmPVN內(nèi)41%以及mpPVN內(nèi)9%表達(dá)nesfatin-1;在腦干,腹部手術(shù)可分別激活LC內(nèi)91%、rRPa內(nèi)82%、EW和VLM內(nèi)74%、NTS14%表達(dá)c-Fos的iesfatin-1神經(jīng)元。 結(jié)論：腹部手術(shù)可激活下丘腦和腦干攝食相關(guān)中樞內(nèi)nesfatin-1神經(jīng)元,其對(duì)攝食和胃排空障礙疾病調(diào)控具有潛在臨床意義。
[Abstract]:Objective: To observe the effect of intestinal obstruction after operation on the expression of nesfatin-1 in the brain of rats.
Methods: abdominal operation was performed after intraperitoneal injection of SEC butyl barbiturate (100 ~ 150mg/kg). After 2 hours, the expression of c-Fos and nesfatin-1 immunoreactive neurons in rat brain was observed by immunohistochemistry.
Results: the hypothalamic supraoptic nucleus of rats in operation group (SON) (t=6.9, P0.01), paraventricular nucleus (mpPVN (t=17.2; P0.01), mmPVN (t=25.8, P0.01), 1mPVN (t=19.5; P0.01) and apPVN (t==22.1; P0.01)), blue (LC) (t=2.6, P0.05), oculomotor nerve accessory nucleus (EW) (t=10.74, P0.01), nucleus raphe pallidus (rRPa) (t=2.57, P0.05), the nucleus of the solitary tract (NTS) (t=10.46, P0.01), ventrolateral medulla (VLM) (t=7.86, P0.01) expression of c-Fos neurons increased significantly, but the expression of nesfatir-1 immunoreactive neurons did not change significantly (P0.05). But the statistical analysis found that the activation of c-Fos neurons in abdominal surgery, SON in the hypothalamus in 99%, apPVN in 71%, 1mPVN in 47%, mmPVN 41% and mpPVN 9% expression of nesfatin-1; in the brainstem, abdominal surgery can activate LC in 91%, rRPa in 82%, EW and VLM in 74%, NTS14% expression of c-Fos iesfatin-1 neurons.
Conclusion: abdominal operation can activate nesfatin-1 neurons in hypothalamic and brainstem feeding related central nervous system, and it has potential clinical significance in regulating the disease of ingestion and gastric emptying disorders.

【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R574.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 Silvia Sookoian;Gustavo Casta濼o;Carolina Gemma;Tomas Fernández Gianotti;Carlos Jose Pirola;;Common genetic variations in CLOCK transcription factor are associated with nonalcoholic fatty liver disease[J];World Journal of Gastroenterology;2007年31期

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本文編號(hào)：1426942