The imbalance between β-amyloid(Aβ) generation and clearance plays a fundamental role in the pathogenesis of Alzheimer ’s disease(AD). The sporadic form of AD is characterized by an overall impairment in Aβ clearance. Immunotherapy targeting Aβ clearance is believed to be a promising approach and is under active clinical investigation. Autophagy is a conserved pathway for degrading abnormal protein aggregates and is crucial for Aβ clearance. We previously reported that oral vaccination with a re...

【文章頁(yè)數(shù)】：14 頁(yè)

【文章目錄】：
INTRODUCTION
MATERIALS AND METHODS
    Construction of the Vaccine
    Cell Culture and Treatment
    Animals and Oral Vaccination
    Sample Preparation
ELISA
    Detection of Serum Anti-Aβ Antibody and Characterization of Antibody Isotypes
    Immunof luorescence Imaging
    Western Blotting Analysis
    Statistical Analysis
RESULTS
    r AAV/Aβ Vaccine Transfection Increased Aβ Secretion in HEK293 Cells
    Oral Vaccination with r AAV/Aβ Signif icantly Attenuated Brain Aβ Burden in APP/PS1 Transgenic Mice
    O r a l V a c c i n a t i o n w i t h r A A V / A β I n c r e a s e d Concentrations of Serum Anti-A β Antibody and Elevated Brain Complement Levels in APP/PS1 Mice
    Activation of Autophagy Function in Brains of APP/PS1Mice with Oral r AAV/Aβ Vaccination
    Akt/m TOR Pathway Suppressed in Brains of APP/PS1Mice with Oral r AAV/Aβ Vaccination
DISCUSSION
    Oral Vaccination with r AAV/Aβ Initiated the Immune Response and Increased Serum Anti-Aβ Antibody in APP/PS1 Mice
    Potential Mechanisms Responsible for Oral VaccinationInduced Antibody- Mediated Aβ Clearance
    Enhancement of Autophagy in the Brain May Play a Novel Role in Oral Vaccination-Induced Aβ Clearance
    Oral Vaccination Appears to Be a Safe Strategy for the Early Prevention of AD



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