本文選題：甘丙肽　切入點(diǎn)：甘丙肽受體　出處：《中國(guó)神經(jīng)精神疾病雜志》2017年07期

【摘要】：目的研究在雌性焦慮抑郁樣行為小鼠海馬中甘丙肽及其受體的表達(dá)和所起的作用。方法制備甘丙肽及甘丙肽受體-1(galanin receptor subtybe 1,Gal R1)、受體-2(Gal R2)、受體-3(Gal R3)探針,運(yùn)用原位雜交技術(shù)觀察甘丙肽及其受體Gal R1、Gal R2、Gal R3在雌性C57BL/6J小鼠海馬中分布情況。另取雌性C57BL/6J小鼠隨機(jī)分為實(shí)驗(yàn)組(15只)與對(duì)照組(15只),實(shí)驗(yàn)組給予慢性溫和型不可預(yù)見性刺激(chronic unpredictability mild stress,CUMS)4周,對(duì)照組不給予任何刺激。4周后分別行糖水偏好實(shí)驗(yàn)、新物體識(shí)別實(shí)驗(yàn)、曠場(chǎng)實(shí)驗(yàn)及懸尾實(shí)驗(yàn)以評(píng)估小鼠的焦慮抑郁樣行為水平及工作記憶情況;行為實(shí)驗(yàn)后,每組8只小鼠提取海馬RNA行qPCR以檢測(cè)海馬甘丙肽及其受體表達(dá)情況,其余小鼠取腦行免疫熒光染色檢測(cè)海馬C-Fos陽性細(xì)胞數(shù)。結(jié)果原位雜交結(jié)果顯示甘丙肽及其受體Gal R1、Gal R2在腹側(cè)海馬Hilus區(qū)有表達(dá),Gal R3未見陽性信號(hào)。糖水偏好實(shí)驗(yàn)實(shí)驗(yàn)組較對(duì)照組糖水飲用量減少(P0.05);新物體識(shí)別實(shí)驗(yàn)顯示,實(shí)驗(yàn)組比對(duì)照組探索新物體時(shí)間百分比短(P0.05);曠場(chǎng)實(shí)驗(yàn)中,實(shí)驗(yàn)組小鼠較對(duì)照組中間停留時(shí)間縮短(P0.05);懸尾實(shí)驗(yàn)中,實(shí)驗(yàn)組較對(duì)照組累計(jì)靜止時(shí)間延長(zhǎng)(P0.05)。qPCR結(jié)果示實(shí)驗(yàn)組小鼠海馬甘丙肽及Gal R1表達(dá)較對(duì)照組增高(P0.05)。免疫組化結(jié)果示實(shí)驗(yàn)組小鼠海馬齒狀回C-Fos陽性細(xì)胞較對(duì)照組增多(P0.05)。結(jié)論甘丙肽、Gal R1及Gal R2主要分布在小鼠海馬的腹側(cè)Hillus區(qū);在雌性C57BL/6J小鼠中,海馬甘丙肽及Gal R1的增加可能與焦慮抑郁樣行為有關(guān),與海馬齒狀回C-Fos細(xì)胞增加也有關(guān)。
[Abstract]:Aim to study the expression and role of glycine and its receptor in the hippocampus of female anxiety and depression like mice.Methods the glianin and its receptor -1galanin receptor subtybe 1G al R1, receptor -2n Gal R2, receptor -3 Gal R3 were prepared. The distribution of galanin and its receptor Gal R1, Gal R2Gal R3 in the hippocampus of female C57BL/6J mice were observed by in situ hybridization (ish) technique. The distribution of galanin and its receptor, Gal R1, Gal R2Gal R3 in the hippocampus of female C57BL/6J mice were observed by in situ hybridization.In addition, female C57BL/6J mice were randomly divided into experimental group (n = 15) and control group (n = 15). The experimental group was given chronic unpredictability mild stress (CUMS) for 4 weeks, and the control group was given sugar water preference experiment after 4 weeks.New object recognition test, open field test and tail suspension test were used to evaluate the anxiety and depression like behavior level and working memory of mice. After the behavioral experiment, 8 mice in each group extracted hippocampal RNA for qPCR to detect the expression of glianin and its receptor in hippocampus.The number of C-Fos positive cells in hippocampus was detected by immunofluorescence staining in other mice.Results the results of in situ hybridization showed that there was no positive expression of Gal R3 in the ventral hippocampal Hilus region.Compared with the control group, the sugary water preference experiment group had less sugar water consumption than the control group, and the new object recognition experiment showed that the experimental group had shorter time to explore the new object than the control group (P 0.05); in the open field experiment,Compared with the control group, the median residence time of the experimental group was shorter than that of the control group (P 0.05), and the cumulative resting time of the experimental group was longer than that of the control group. The results of qPCR showed that the expression of glianin and Gal R1 in the hippocampus of the experimental group was higher than that of the control group (P 0.05).Immunohistochemical results showed that the number of C-Fos positive cells in dentate gyrus of experimental group was higher than that of control group (P 0.05).Conclusion Gal R1 and Gal R2 are mainly distributed in the ventral Hillus region of the hippocampus of mice, and the increase of Gal R1 and Gal R1 in female C57BL/6J mice may be related to anxiety and depressive behavior, as well as to the increase of C-Fos cells in dentate gyrus.
【作者單位】： 第四軍醫(yī)大學(xué)附屬唐都醫(yī)院神經(jīng)內(nèi)科;
【基金】：國(guó)家自然科學(xué)基金(編號(hào):31371094)
【分類號(hào)】：R749.4

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本文編號(hào)：1721032