目的:煙草煙霧暴露下建立小鼠肺氣腫模型,觀察調(diào)節(jié)性T細(xì)胞（regulatory T cell,Treg）中組蛋白去乙�；福╤istone deacetylase,HDAC）活性對Treg/Th17細(xì)胞的平衡變化,探討吸煙導(dǎo)致肺氣腫中存在CD4⁺T細(xì)胞亞群之間相互失衡的機(jī)制。方法:將健康雄性8周齡Balb/c小鼠按照隨機(jī)數(shù)字法分為對照組、煙熏組,每組各30只,對照組暴露于新鮮空氣中自然喂養(yǎng)24周,煙熏組用香煙煙霧暴露24周建立小鼠肺氣腫模型。（1）蘇木精-伊紅（HE）染色觀察小鼠肺組織形態(tài)學(xué)的改變,計(jì)算兩組肺泡平均內(nèi)襯間隔（Mean linear intercepts,MLI）。（2）流式細(xì)胞術(shù)檢測小鼠外周血、脾臟和肺組織中CD4⁺、CD4⁺IL-17、CD4⁺IFN-γ、CD4⁺Foxp3⁺細(xì)胞的表達(dá)。（3）采用免疫磁珠法分選肺組織中Treg細(xì)胞,實(shí)時(shí)熒光定量PCR法（Real-time polymerase chain reaction,RT-PCR）... 

【文章來源】：廣西醫(yī)科大學(xué)廣西壯族自治區(qū)

【文章頁數(shù)】：85 頁

【學(xué)位級別】：碩士

【部分圖文】：

A為對照組，B為煙熏組（HE×100）

圖 2：A 圖為對照組、煙熏組小鼠外周血、脾臟、肺組織中的 CD4 細(xì)胞表達(dá)水平；B 圖為對照組、煙熏組小鼠外周血、脾臟、肺組織的 CD4+Foxp3+細(xì)胞表達(dá)水平（*P<0.05）。Figure2，The ratio of CD4+, CD4+Foxp3+cells in peripheral blood, spleen and lung(*P<0.05).Figure2 Notes: There was no significant change in the expression ratio of CD4 cells.The ratio ofCD4+Foxp3+ Treg cells, from peripheral blood, spleen and lungs in cigarette smoke exposure group wereincreased compared with those in control group (P<0.05).

2：A 圖為對照組、煙熏組小鼠外周血、脾臟、肺組織中的 CD4 細(xì)胞表達(dá)水平；B 圖為對照組、煙熏組小鼠外周血、脾臟、肺組織的 CD4+Foxp3+細(xì)胞表達(dá)水平（*P<0.05）。Figure2，The ratio of CD4+, CD4+Foxp3+cells in peripheral blood, spleen and lung(*P<0.05).igure2 Notes: There was no significant change in the expression ratio of CD4 cells.The ratio ofD4+Foxp3+ Treg cells, from peripheral blood, spleen and lungs in cigarette smoke exposure group werecreased compared with those in control group (P<0.05).


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