The anti-inflammatory and antibacterial mechanisms of bone marrow mesenchymal stem cells（MSCs） ameliorating lung injury in chronic obstructive pulmonary disease（COPD） mice induced by cigarette smoke and Haemophilus Parainfluenza（HPi） were studied. The experiment was divided into four groups in vivo: control group, COPD group, COPD+HPi group, and COPD+HPi+MSCs group. The indexes of emphysematous changes, inflammatory reaction and lung injury score, and antibacterial effects were evaluated in all ... 

【文章來源】：Journal of Huazhong University of Science and Technology（Medical Sciences）. 2017,37(04)

【文章頁數(shù)】：9 頁

【文章目錄】：
1 MATERIALS AND METHODS
    1.1 Isolation and Culture of Bone Marrow MSCs
    1.2 HPi Bacterial Cultivation
    1.3 Establishment of COPD Model by Cigarette Smoke and HPi
    1.4 Grouping and Bone Marrow MSCs Transplanta-tion
    1.5 Bronchoalveolar Lavage Fluid and Lung Tissue Collection
    1.6 Cell Count and Classification of Bronchoalveolar Lavage Fluid
    1.7 Lung Morphologic Analysis and Lung Injury Score
    1.8 Quantitative PCR
    1.9 Cytokines of BALF
    1.10 Bacteriological Count in Lung
    1.1 1 Myeloperoxidase （MPO） Activity Assessment of Lung Tissue
    1.1 2 Alveolar Macrophages Cultivation and Phago-cytosis Function Assessment
    1.1 3 Cigarette Smoke Extract （CSE） Preparation
    1.1 4 Anti-inflammatory and Antibacterial Activity of Macrophages Regulated by MSCs In Vitro
    1.1 5 Western Blot Analysis of NF-кB p65 and CD206
    1.16 Statistical Analysis
2 RESULTS
    2.1 MSCs Transplantation Mitigating Emphysema-tous Changes and Lung Injury Score in COPD Mice Induced by HPi
    2.2 MSCs Transplantation Mitigating Inflammation in COPD Mice Induced by HPi and Cigarette Smoke
    2.3 MSCs Transplantation Promotes Bacterial Clearance in Lung
    2.4 MSCs Transplantation Promotes Phagocytosis of Macrophages in BALF
    2.5 Anti-inflammatory and Antibacterial Activity of Macrophages Regulated by MSCs In Vitro
3 DISCUSSION
Conflict of Interest Statement


【參考文獻(xiàn)】：
期刊論文
[1]MSCs Relieve Lung Injury of COPD Mice through Promoting Proliferation of Endogenous Lung Stem Cells[J]. 劉紅梅,馬利軍,吳紀(jì)珍,李玉光.  Journal of Huazhong University of Science and Technology（Medical Sciences）. 2015(06)
[2]Apoptosis of Alveolar Wall Cells in Chronic Obstructive Pulmonary Disease Patients with Pulmonary Emphysema Is Involved in Emphysematous Changes[J]. 劉紅梅,馬利軍,吳紀(jì)珍,王凱,陳獻(xiàn)亮.  Journal of Huazhong University of Science and Technology（Medical Sciences）. 2009(04)
[3]骨髓間充質(zhì)干細(xì)胞移植對(duì)木瓜蛋白酶和鈷60照射所致肺氣腫大鼠肺泡壁細(xì)胞凋亡的影響[J]. 劉紅梅,甄國華,張珍祥,張惠蘭,曹勇,汪濤,顧乃兵,徐永健.  中國應(yīng)用生理學(xué)雜志. 2008(02)



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