【摘要】：取1只妊娠17.5 d的ICR小鼠,將其體內(nèi)的胎鼠肺泡Ⅱ型上皮細(xì)胞(AECⅡ)經(jīng)分離、純化、培養(yǎng)、鑒定后,分別加入不同濃度的神經(jīng)酰胺,培養(yǎng)12、24和48 h后進(jìn)行MTT試驗(yàn)。在Transwell上構(gòu)建AECⅡ單層,以10μmol/L的神經(jīng)酰胺與AECⅡ培養(yǎng)12和24 h,并設(shè)置相應(yīng)對照組,應(yīng)用伏歐計(jì)測定跨上皮電阻值。結(jié)果顯示,隨著神經(jīng)酰胺染毒濃度的升高和染毒時間的延長,AECⅡ細(xì)胞增殖抑制率呈上升趨勢。10μmol/L的神經(jīng)酰胺分別刺激融合的AECⅡ單層12和24 h后,與未處理對照組比較,神經(jīng)酰胺刺激組的跨上皮電阻值顯著下降。
[Abstract]:One ICR mouse with 17.5d gestation was isolated, purified, cultured and identified by isolation, purification, culture and identification of alveolar type II epithelial cells (AEC II). Different concentrations of ceramide were added and cultured for 12, 24 and 48 hours, respectively. MTT test was carried out after 24 and 48 hours of culture. AEC 鈪，

本文編號：2500908