血清IL-21、CXCL13水平與慢性阻塞性肺疾病的相關(guān)性研究
發(fā)布時間：2019-02-23 12:26
【摘要】：目的探討患者血清中IL-21、CXCL13水平與慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)不同疾病狀態(tài)的相關(guān)性及其臨床意義。方法收集20例健康非吸煙者、20例健康吸煙者、31例COPD穩(wěn)定期患者和30例COPD急性加重期(acute exacerbation of COPD,AECOPD)患者的靜脈血,經(jīng)離心后取上清采用ELISA法檢測血清中IL-21、CXCL13的水平,應(yīng)用SPSS 21.0統(tǒng)計軟件,采用單因素方差分析以及Pearson相關(guān)分析等方法,分析比較4組間IL-21、CXCL13水平的差異及相關(guān)性,并同時檢測COPD穩(wěn)定期組患者的肺功能及GOLD分級,分析肺功能、GOLD分級分別與IL-21、CXCL13水平的相關(guān)性。結(jié)果 AECOPD組患者血清中IL-21、CXCL13水平均明顯高于COPD穩(wěn)定期組、健康吸煙組、健康非吸煙組(P0.05);COPD穩(wěn)定期組患者血清IL-21、CXCL13水平與健康吸煙組差異無統(tǒng)計學(xué)意義(P0.05),但明顯高于健康非吸煙組(P0.05);健康吸煙組血清中IL-21、CXCL13水平顯著高于健康非吸煙組(P0.05)。COPD穩(wěn)定期組血清IL-21、CXCL13水平與患者肺功能中FEV1值呈負(fù)相關(guān)(r分別為-0.619、-0.505,P0.05),并與患者GOLD分級呈正相關(guān)(r分別為0.901、0.458,P0.05)。COPD穩(wěn)定期患者血清中IL-21水平與CXCL13水平呈正相關(guān)(r=0.424,P0.05)。結(jié)論健康吸煙人群、COPD穩(wěn)定期和急性加重期患者血清中IL-21、CXCL13水平顯著上升;COPD穩(wěn)定期患者血清中IL-21、CXCL13水平與肺功能和疾病嚴(yán)重程度相關(guān);IL-21、CXCL13可能在COPD進(jìn)程中發(fā)揮促炎作用。
[Abstract]:Objective to investigate the relationship between serum IL-21,CXCL13 level and different disease states of chronic obstructive pulmonary disease (chronic obstructive pulmonary disease,COPD) and its clinical significance. Methods the venous blood samples from 20 healthy non-smokers, 20 healthy smokers, 31 stable COPD patients and 30 (acute exacerbation of COPD,AECOPD patients with acute exacerbation of COPD were collected. After centrifugation, the serum IL-21, was detected by ELISA method. The level of CXCL13 was analyzed and compared by using SPSS 21.0 software, single factor variance analysis and Pearson correlation analysis. At the same time, the pulmonary function and GOLD grade of COPD stable group were detected, and the correlation between pulmonary function and GOLD grade and IL-21,CXCL13 level were analyzed. Results the serum IL-21,CXCL13 levels in AECOPD group were significantly higher than those in stable COPD group, healthy smoking group and healthy non-smoking group (P0.05). There was no significant difference in serum IL-21,CXCL13 level between COPD stable group and healthy smoking group (P0.05), but it was significantly higher than that of healthy non-smoking group (P0.05). The level of serum IL-21,CXCL13 in the healthy smoking group was significantly higher than that in the healthy non-smoking group (P0.05). The serum IL-21,CXCL13 level in the stable). COPD group was negatively correlated with the value of FEV1 in the lung function of the patients (r = -0.619 ~ -0.505, respectively). There was a positive correlation between the serum IL-21 level and the CXCL13 level in the patients with stable). COPD (r = 0.901U 0.458P, respectively) (r = 0.424, P 0.05), and there was a positive correlation between the serum IL-21 level and the CXCL13 level (r = 0.901 / 0.458g, P < 0.05). Conclusion the serum IL-21,CXCL13 levels in healthy smokers, COPD stable and acute exacerbation patients were significantly increased, and the serum IL-21,CXCL13 levels were correlated with lung function and severity of disease in COPD stable patients. IL-21,CXCL13 may play an inflammatory role in the process of COPD.
【作者單位】： 第三軍醫(yī)大學(xué)新橋醫(yī)院呼吸內(nèi)科 全軍呼吸內(nèi)科研究所 全軍呼吸病研究重點實驗室;
【基金】：國家自然科學(xué)基金面上項目(81570051)~~
【分類號】：R563.9
，
本文編號：2428820
[Abstract]:Objective to investigate the relationship between serum IL-21,CXCL13 level and different disease states of chronic obstructive pulmonary disease (chronic obstructive pulmonary disease,COPD) and its clinical significance. Methods the venous blood samples from 20 healthy non-smokers, 20 healthy smokers, 31 stable COPD patients and 30 (acute exacerbation of COPD,AECOPD patients with acute exacerbation of COPD were collected. After centrifugation, the serum IL-21, was detected by ELISA method. The level of CXCL13 was analyzed and compared by using SPSS 21.0 software, single factor variance analysis and Pearson correlation analysis. At the same time, the pulmonary function and GOLD grade of COPD stable group were detected, and the correlation between pulmonary function and GOLD grade and IL-21,CXCL13 level were analyzed. Results the serum IL-21,CXCL13 levels in AECOPD group were significantly higher than those in stable COPD group, healthy smoking group and healthy non-smoking group (P0.05). There was no significant difference in serum IL-21,CXCL13 level between COPD stable group and healthy smoking group (P0.05), but it was significantly higher than that of healthy non-smoking group (P0.05). The level of serum IL-21,CXCL13 in the healthy smoking group was significantly higher than that in the healthy non-smoking group (P0.05). The serum IL-21,CXCL13 level in the stable). COPD group was negatively correlated with the value of FEV1 in the lung function of the patients (r = -0.619 ~ -0.505, respectively). There was a positive correlation between the serum IL-21 level and the CXCL13 level in the patients with stable). COPD (r = 0.901U 0.458P, respectively) (r = 0.424, P 0.05), and there was a positive correlation between the serum IL-21 level and the CXCL13 level (r = 0.901 / 0.458g, P < 0.05). Conclusion the serum IL-21,CXCL13 levels in healthy smokers, COPD stable and acute exacerbation patients were significantly increased, and the serum IL-21,CXCL13 levels were correlated with lung function and severity of disease in COPD stable patients. IL-21,CXCL13 may play an inflammatory role in the process of COPD.
【作者單位】： 第三軍醫(yī)大學(xué)新橋醫(yī)院呼吸內(nèi)科 全軍呼吸內(nèi)科研究所 全軍呼吸病研究重點實驗室;
【基金】：國家自然科學(xué)基金面上項目(81570051)~~
【分類號】：R563.9
，
本文編號：2428820
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