【摘要】：目的研究哮喘小鼠體內(nèi)Tfh細(xì)胞以及Bcl-6蛋白的變化,并初步探討氨茶堿對(duì)哮喘的治療作用。方法 4~6周齡BALB/c小鼠40只,隨機(jī)分成4組,每組10只。分別為正常對(duì)照組、哮喘模型組、氨茶堿治療組及地塞米松陽性對(duì)照組。后3組建立哮喘模型后對(duì)應(yīng)給以基礎(chǔ)處置。按兩因素析因設(shè)計(jì)測(cè)取小鼠氣道反應(yīng)性資料,HE染色觀察小鼠肺組織的病理形態(tài),流式細(xì)胞術(shù)檢測(cè)Tfh細(xì)胞的比例變化,Western-blotting檢測(cè)Bcl-6的蛋白表達(dá)量,Elisa法測(cè)定肺泡灌洗液以及血清中IL-4、IL-10的水平。結(jié)果哮喘模型組小鼠的氣道阻力較正常組小鼠顯著升高(P0.05),HE染色切片中氣道平滑肌增厚,且有大量的炎癥細(xì)胞浸潤(rùn),炎癥評(píng)分較正常組明顯升高(P0.05),小鼠脾臟中的Tfh細(xì)胞的比例較正常對(duì)照組小鼠顯著升高,Bcl-6蛋白含量較正常組明顯升高,血清以及肺泡灌洗液中IL-4含量較正常對(duì)照組顯著升高,而IL-10的水平顯著下降;而氨茶堿治療組以及地塞米松陽性對(duì)照組的氣道阻力較哮喘模型組小鼠顯著降低(P0.05),小鼠HE染色切片發(fā)現(xiàn)其炎癥細(xì)胞浸潤(rùn)程度較哮喘模型組顯著降低,Tfh細(xì)胞的比例較哮喘模型組顯著降低,肺組織中Bcl-6蛋白表達(dá)水平較哮喘模型組顯著降低,血清以及肺泡灌洗液中IL-4含量較哮喘模型組明顯下降,IL-10水平顯著升高。結(jié)論哮喘小鼠Tfh細(xì)胞比例增加,Bcl-6蛋白水平升高,氨茶堿可以有效改善哮喘小鼠氣道炎癥和氣道高反應(yīng)性,可能與其影響Tfh細(xì)胞比例以及IL-4、IL-10的水平有關(guān)。
[Abstract]:Objective to study the changes of Tfh cells and Bcl-6 protein in asthmatic mice and to explore the therapeutic effect of aminophylline on asthma. Methods 40 BALB/c mice aged 4 weeks 6 weeks were randomly divided into 4 groups with 10 mice in each group. They were normal control group, asthma model group, aminophylline treatment group and dexamethasone positive control group. The asthmatic model was established in the latter three groups. The airway reactivity of mice was measured by two factor factorial design. HE staining was used to observe the pathological morphology of mouse lung tissue, flow cytometry was used to detect the proportion of Tfh cells, and Western-blotting was used to detect the protein expression of Bcl-6. The levels of IL-4,IL-10 in alveolar lavage fluid and serum were measured by Elisa method. Results the airway resistance in asthmatic model group was significantly higher than that in normal group (P0.05). The airway smooth muscle was thickened and a large number of inflammatory cells infiltrated in the), HE staining section, and the inflammatory score was significantly higher than that in the normal group (P0.05). The percentage of Tfh cells in the spleen of mice was significantly higher than that of the control group, the content of Bcl-6 protein was significantly higher than that of the normal group, and the content of IL-4 in serum and alveolar lavage fluid was significantly higher than that in the normal control group. However, the level of IL-10 decreased significantly. The airway resistance in the aminophylline treatment group and the dexamethasone positive control group was significantly lower than that in the asthma model group (P0.05). The degree of inflammatory cell infiltration in the HE staining section of the mice was significantly lower than that in the asthmatic model group. The proportion of Tfh cells was significantly lower than that of asthma model group, the expression of Bcl-6 protein in lung tissue was significantly lower than that in asthmatic model group, the content of IL-4 in serum and alveolar lavage fluid was significantly lower than that in asthma model group, and the level of IL-10 was significantly higher than that in asthmatic model group. Conclusion Aminophylline can effectively improve airway inflammation and airway hyperresponsiveness in asthmatic mice by increasing the proportion of Tfh cells and the level of Bcl-6 protein, which may be related to the effect of aminophylline on the proportion of Tfh cells and the level of IL-4,IL-10.
【作者單位】： 河北省唐山市工人醫(yī)院呼吸科;河北聯(lián)合大學(xué)附屬醫(yī)院呼吸內(nèi)科;
【分類號(hào)】：R562.25
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本文編號(hào)：2410929