【摘要】：目的:探討正常組與人工模擬寒環(huán)境組大鼠肺組織蛋白組學(xué)差異表達(dá)及質(zhì)譜分析。方法:選取20只SD大鼠隨機(jī)分為正常對(duì)照組與寒環(huán)境組,每組10只。寒環(huán)境組模型復(fù)制使用人工氣候箱:(T)=(6±2)℃,每天刺激4 h(其余時(shí)間為正常組溫濕度);正常組(T)=(21±2)℃,每天持續(xù)24 h。造模7 d后,兩組大鼠于麻醉前12 h禁食、禁水,稱量體重。麻醉后開胸取出左肺組織10 mg/只,每組取3個(gè)樣本進(jìn)行樣品蛋白質(zhì)制備、雙向電泳實(shí)驗(yàn)、圖像分析、質(zhì)譜檢測(cè)。結(jié)果:對(duì)兩組差異蛋白圖譜進(jìn)行蛋白質(zhì)組學(xué)分析,鑒定出9個(gè)蛋白在兩組之間差異表達(dá),寒環(huán)境組與正常對(duì)照組比較,其中4個(gè)表達(dá)上調(diào)、5個(gè)表達(dá)下調(diào)。結(jié)論:通過(guò)蛋白質(zhì)組學(xué)分析,鑒定出的蛋白與外寒環(huán)境有相關(guān)性。
[Abstract]:Aim: to investigate the differential expression of proteomics and mass spectrometry in lung tissue between the normal group and the artificial cold environment group. Methods: twenty SD rats were randomly divided into normal control group and cold environment group with 10 rats in each group. The model of cold environment group was duplicated with artificial climate box: (T) = (6 鹵2) 鈩，

本文編號(hào)：2368163