【摘要】：目的檢測(cè)不同臨床分期塵肺患者血清中miR-21的表達(dá)水平,探討其作為塵肺分子標(biāo)志物的可能性。方法采集118例男性塵肺患者(Ⅰ期57例、Ⅱ期48例和Ⅲ期13例)靜脈血標(biāo)本,收集血清,用實(shí)時(shí)熒光定量RT-PCR法檢測(cè)其miR-21的表達(dá)水平,并以115例年齡、性別匹配的健康人作為對(duì)照。結(jié)果健康對(duì)照者血清miR-21的相對(duì)表達(dá)量為(0.004±0.003),Ⅰ期和Ⅱ~Ⅲ期塵肺患者分別為(0.011±0.008)和(0.022±0.018)。Ⅰ期和Ⅱ~Ⅲ期塵肺患者血清miR-21水平顯著高于健康對(duì)照組(t分別為-6.39,-7.81,P均0.01)。且Ⅱ~Ⅲ期患者高于Ⅰ期(t=-4.28,P0.01)。用于塵肺診斷時(shí)miR-21的ROC曲線下面積(AUCROC)為0.85(95%CI:0.78~0.93),以相對(duì)表達(dá)量0.007為臨界值,診斷塵肺的敏感性和特異性分別為83.9%和90.4%。結(jié)論 miR-21在塵肺患者血清中高表達(dá),且與臨床分期有關(guān),有望成為用于塵肺診斷的分子標(biāo)志物。
[Abstract]:Objective to detect the expression of miR-21 in serum of patients with pneumoconiosis at different clinical stages and to explore the possibility of using it as a molecular marker of pneumoconiosis. Methods the venous blood samples were collected from 118 male patients with pneumoconiosis (57 cases in stage 鈪，

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