Pre-miRNA單核苷酸多態(tài)性與慢性阻塞性肺疾病的相關(guān)性研究
發(fā)布時(shí)間：2018-11-18 08:51
【摘要】：研究目的： 采用病例-對照研究方法探索pre-miRNA基因多態(tài)性與中國甘肅地區(qū)漢族人群慢性阻塞性肺疾病之間的相關(guān)性。包括pre-miRNA基因中SNP位點(diǎn)rs2910164G/C、rs11614913C/T、 rs3746444C/T。 研究方法： 在蘭州大學(xué)第一醫(yī)院和四川大學(xué)華西醫(yī)院篩選532例沒有血緣關(guān)系的臨床診斷明確的COPD患者和511例正常對照人群。收集COPD患者及正常對照人群血液樣本,采用離心柱法提取樣本的全基因組DNA,用紫外分光光度儀測定DNA的濃度,然后設(shè)計(jì)位點(diǎn)特異性擴(kuò)增引物,利用聚合酶鏈?zhǔn)椒磻?yīng)和限制性片段長度多態(tài)性(PCR-RFLP)的方法分析了3個(gè)位于pre-miRNA基因的單核苷酸位點(diǎn)(rs11614913, rs3746444, and rs2910164)在中國漢族人群COPD患者和正常人中的等位基因和基因型頻率,使用統(tǒng)計(jì)學(xué)軟件SPSS11.5分析兩者的差異。 結(jié)果： SNP位點(diǎn)rs11614913C/T和rs3746444A/G的等位基因頻率和基因型頻率在病例組和對照組之間的分布有顯著的差異。rs11614913C/T多態(tài)性的CC基因型和C等位基因和COPD發(fā)病增高顯著相關(guān)(CC對比IT, OR=0.55,95%CI,0.372-0.818;C對比T,OR=0.806,95%CI,0.676-0.962),類似的,rs3746444A/G多態(tài)性的AA基因型和A等位基因也表現(xiàn)出了與COPD發(fā)病增高很強(qiáng)的相關(guān)性(AA對比GG,OR=0.158,95%CI,0.046-0.542;A對比G, OR=0.733,95%CI,0.569-0.943).然而,本研究的另外一個(gè)多態(tài)性位點(diǎn)：rs2910164G/C的等位基因頻率和基因型頻率在病例組和對照之間的并沒有表現(xiàn)出明顯的差異。 結(jié)論： 研究顯示rs11614913C/T和rs3746444A/G多態(tài)性在COPD的發(fā)病過程中起到重要作用,提示pre-miRNA hsa-mir-196a和hsa-mir-499基因在中國漢族人群中與COPD的發(fā)病有很強(qiáng)的相關(guān)性,而pre-miRNA hsa-mir-146a并沒有表現(xiàn)出與COPD發(fā)病的相關(guān)性。
[Abstract]:Objective: to investigate the association between pre-miRNA gene polymorphism and chronic obstructive pulmonary disease (COPD) in Han population in Gansu province of China. Including SNP rs2910164G/C,rs11614913C/T, rs3746444C/T. in the pre-miRNA gene Methods: 532 COPD patients and 511 normal controls were selected from the first Hospital of Lanzhou University and Huaxi Hospital of Sichuan University. Blood samples from patients with COPD and normal controls were collected. The concentration of DNA was determined by UV spectrophotometry, and then locus specific primers were designed. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (PCR-RFLP) were used to analyze three single nucleotide loci (rs11614913, rs3746444,) of pre-miRNA gene. And rs2910164) alleles and genotype frequencies in COPD patients and normal controls were analyzed by SPSS11.5 software. Results: the allele frequency and genotype frequency of rs11614913C/T and rs3746444A/G at SNP locus were significantly different between case group and control group. CC genotype and C allele and COPD were increased in rs11614913C/T polymorphism. High significant correlation (CC contrast IT,) OR=0.55,95%CI,0.372-0.818; Similarly, the AA genotypes and A alleles of rs3746444A/G polymorphisms also showed a strong correlation with the increased incidence of COPD (AA versus GG,OR=0.158,95%CI,). 0.046-0.542; A contrast G, OR=0.733,95%CI,0.569-0.943). However, there was no significant difference in allelic and genotypic frequencies between the case group and the control group at another polymorphic locus in this study: rs2910164G/C. Conclusion: rs11614913C/T and rs3746444A/G polymorphisms play an important role in the pathogenesis of COPD, suggesting that pre-miRNA hsa-mir-196a and hsa-mir-499 genes are strongly associated with COPD in Chinese Han population. Pre-miRNA hsa-mir-146a did not show a correlation with the onset of COPD.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R563
本文編號：2339536
[Abstract]:Objective: to investigate the association between pre-miRNA gene polymorphism and chronic obstructive pulmonary disease (COPD) in Han population in Gansu province of China. Including SNP rs2910164G/C,rs11614913C/T, rs3746444C/T. in the pre-miRNA gene Methods: 532 COPD patients and 511 normal controls were selected from the first Hospital of Lanzhou University and Huaxi Hospital of Sichuan University. Blood samples from patients with COPD and normal controls were collected. The concentration of DNA was determined by UV spectrophotometry, and then locus specific primers were designed. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (PCR-RFLP) were used to analyze three single nucleotide loci (rs11614913, rs3746444,) of pre-miRNA gene. And rs2910164) alleles and genotype frequencies in COPD patients and normal controls were analyzed by SPSS11.5 software. Results: the allele frequency and genotype frequency of rs11614913C/T and rs3746444A/G at SNP locus were significantly different between case group and control group. CC genotype and C allele and COPD were increased in rs11614913C/T polymorphism. High significant correlation (CC contrast IT,) OR=0.55,95%CI,0.372-0.818; Similarly, the AA genotypes and A alleles of rs3746444A/G polymorphisms also showed a strong correlation with the increased incidence of COPD (AA versus GG,OR=0.158,95%CI,). 0.046-0.542; A contrast G, OR=0.733,95%CI,0.569-0.943). However, there was no significant difference in allelic and genotypic frequencies between the case group and the control group at another polymorphic locus in this study: rs2910164G/C. Conclusion: rs11614913C/T and rs3746444A/G polymorphisms play an important role in the pathogenesis of COPD, suggesting that pre-miRNA hsa-mir-196a and hsa-mir-499 genes are strongly associated with COPD in Chinese Han population. Pre-miRNA hsa-mir-146a did not show a correlation with the onset of COPD.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R563
【參考文獻(xiàn)】
相關(guān)期刊論文 前2條
1 馬志明,張珍祥,韓澤民,徐永健;GSTM1和GSTT1基因多態(tài)性與COPD易感性的研究[J];中國醫(yī)師雜志;2004年06期
2 周敏,黃紹光,萬歡英,李彪,李敏,程齊儉;中國南方漢族人MMP-9基因多態(tài)性與COPD易感性的關(guān)系[J];上海第二醫(yī)科大學(xué)學(xué)報(bào);2004年02期
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