【摘要】：目的探討矽肺患者肺泡巨噬細胞(AM)表面A類清道夫受體(SR-A)的表達及其對AM凋亡的調(diào)控作用,為矽肺的防治提供依據(jù)。方法以不同期別的矽肺患者51例為調(diào)查對象,以4例矽肺觀察對象為對照組,收集其大容量肺灌洗回收液,分離、純化AM,將純化后的AM分為5組:未處理組、酪氨酸激酶抑制劑組、活性氧抑制劑組、抗-MARCO組和抗-MSR1組,37℃處理24h后,裂解各組AM,采用Western blot方法檢測受體蛋白MSR1和MARCO及凋亡蛋白Caspase-3的表達并計算其相對水平。結(jié)果矽肺患者AM中Caspase-3的表達水平均高于對照組,且隨著肺纖維化程度的加重逐漸增高(P0.05)。MSR1和MARCO表達水平雖有隨矽肺期別增高而增加的趨勢,但差異無統(tǒng)計學(xué)意義(P0.05)。未脫塵組Caspase-3的表達水平高于脫塵組(P0.05);MSR1、MARCO表達水平與Caspase-3均呈正相關(guān)�？�-MARCO和抗-MSR1抗體處理后,矽肺患者Caspase-3的表達均高于未處理組,貳期矽肺患者MSR1表達高于未處理組,貳期和叁期矽肺患者MARCO的表達均較未處理組上調(diào)(P0.05)。貳期、叁期矽肺患者的AM經(jīng)N-乙酰半胱胺酸處理后Caspase-3表達水平下調(diào)。結(jié)論 SR-A在介導(dǎo)矽肺患者AM凋亡中可能發(fā)揮重要的作用。
[Abstract]:Objective to investigate the expression of class A scavenger receptor (SR-A) on alveolar macrophages (AM) and its role in regulating AM apoptosis in silicosis patients, and to provide evidence for the prevention and treatment of silicosis. Methods 51 patients with silicosis and 4 patients with silicosis as control group were divided into 5 groups: untreated group. The large volume lung lavage and recovery solution was collected. The purified AM was separated into 5 groups. Tyrosine kinase inhibitor group, reactive oxygen species inhibitor group, anti-MARCO group and anti-MSR1 group were treated at 37 鈩，

本文編號：2330949