【摘要】：目的探討痰上清液基質(zhì)金屬蛋白酶(MMP)-9及基質(zhì)金屬蛋白酶組織抑制劑(TIMP-1)與慢性阻塞性肺疾病(COPD)氣道炎癥的關(guān)系。方法 COPD急性期患者47例,COPD穩(wěn)定期患者50例,同期健康體檢者50例為健康對(duì)照組,均采集痰液計(jì)算痰細(xì)胞總數(shù),離心處理后經(jīng)細(xì)胞涂片瑞氏染色計(jì)算每類細(xì)胞數(shù),包括中性粒細(xì)胞(Neu)、淋巴細(xì)胞(Lym)、巨噬細(xì)胞(Macro)及嗜酸性細(xì)胞(Eos),同時(shí)采用雙抗體夾心法對(duì)痰上清液MMP-9與TIMP-1進(jìn)行檢測(cè),分析COPD急性期MMP-9、TIMP-1與氣道炎癥的相關(guān)性。結(jié)果 COPD患者的細(xì)胞總數(shù)、Neu、Lym、Macro、Eos明顯高于對(duì)照組(P0.05),急性期組細(xì)胞總數(shù)、Neu、Neu/白細(xì)胞計(jì)數(shù)(WBC)明顯高于穩(wěn)定期組(P0.05),而Lym/WBC、Macro/WBC、Eos/WBC明顯低于穩(wěn)定期組與對(duì)照組(P0.05);急性期組的痰MMP-9、TIMP-1水平及MMP-9/TIMP-1比值均明顯高于穩(wěn)定期組與對(duì)照組(P0.05),且穩(wěn)定期組明顯高于對(duì)照組(P0.05);COPD急性期痰上清液中除TIMP-1外,MMP-9水平及MMP-9/TIMP-1均與Neu、Lym、Macro及Eos呈正相關(guān)(P0.05)。而COPD穩(wěn)定期僅痰上清液TIMP-1與Macro呈正相關(guān)(P0.05)。結(jié)論 MMP-9與COPD氣道炎癥的關(guān)系緊密,TIMP-1與MMP-9水平失衡可能是COPD急性發(fā)作的重要原因。
[Abstract]:Objective to investigate the relationship between matrix metalloproteinase 9 (MMP) 9) and tissue inhibitor of matrix metalloproteinases (TIMP-1) in sputum supernatant and airway inflammation in chronic obstructive pulmonary disease (COPD). Methods 47 patients with acute COPD, 50 patients with stable COPD and 50 healthy controls were collected from sputum to calculate the total number of sputum cells. (Lym), macrophage (Macro) and eosinophil (Eos), were used to detect MMP-9 and TIMP-1 in sputum supernatant by double antibody sandwich method. MMP-9, in acute stage of COPD was analyzed. Correlation between TIMP-1 and airway inflammation. Results the total number of cells in COPD patients was significantly higher than that in the control group (P0.05). In the acute phase group, the total number of cells and the Neu,Neu/ leukocyte count were significantly higher than those in the stable phase group (P0.05), while Lym/WBC,Macro/WBC, was significantly higher in the acute phase group than in the stable group (P0.05). Eos/WBC was significantly lower than that in stable group and control group (P0.05). The sputum MMP-9,TIMP-1 level and MMP-9/TIMP-1 ratio in the acute phase group were significantly higher than those in the stable phase group and the control group (P0.05), and the stable phase group was significantly higher than the control group (P0.05). The level of MMP-9 and MMP-9/TIMP-1 were positively correlated with Neu,Lym,Macro and Eos except TIMP-1 in the supernatant of phlegm in acute phase of COPD (P0.05). In the stable phase of COPD, only the sputum supernatant TIMP-1 was positively correlated with Macro (P0.05). Conclusion there is a close relationship between MMP-9 and COPD airway inflammation. The imbalance of TIMP-1 and MMP-9 may be the important cause of acute attack of COPD.
【作者單位】： 航空總醫(yī)院呼吸科;航空總醫(yī)院檢驗(yàn)科;
【分類號(hào)】：R563.9

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