【摘要】：目的觀察克拉霉素對激素反應(yīng)性通路中組蛋白去乙�；�2(HDAC2)及糖皮質(zhì)激素受體(GR)的影響,探討克拉霉素對煙霧暴露哮喘小鼠可能的治療作用。方法選擇BALB/c小鼠作為研究對象,通過卵蛋白致敏激發(fā)建立哮喘模型組(OVA組)、煙霧暴露哮喘組(SEA組)以及哮喘接觸煙霧暴露后通過克拉霉素治療組(CAM組),同時設(shè)立對照組平行比較。采用組織病理學(xué)檢測各組小鼠肺組織炎癥情況,并通過PCR檢測肺組織中HDAC2mRNA的表達情況和Western blot檢測肺組織中HDAC2和GR蛋白的表達。結(jié)果組織病理學(xué)觀察OVA組和SEA組均存在肺組織氣道炎癥,其中SEA組纖毛被嚴(yán)重破壞,而CAM組炎癥細胞浸潤減弱。與CAM組相比,SEA組小鼠支氣管肺泡灌洗液中IL-4和IL-8水平均顯著提高(104.36±14.39 vs.65.49±10.82、681.35±66.18 vs.321.49±90.37,P=0.031、0.017)。CAM組HDAC2mRNA表達顯著高于SEA組(0.062±0.013 vs.0.031±0.015,P=0.032)。Western blot檢測結(jié)果顯示,與CAM組相比,SEA組HDAC2蛋白(0.23±0.017 vs.0.49±0.022,P=0.033)和GR蛋白(0.19±0.014 vs.0.64±0.023,P=0.011)表達均顯著降低。結(jié)論克拉霉素能抑制煙霧暴露的哮喘小鼠氣道炎癥反應(yīng),作用機制可能通過與GR相結(jié)合,影響下游HDAC2基因表達有關(guān)。
[Abstract]:Objective to observe the effects of clarithromycin on histone deacetylase 2 (HDAC2) and glucocorticoid receptor (GR) in hormone responsive pathway and to explore the possible therapeutic effects of clarithromycin on smog exposed asthma mice. Methods BALB/c mice were selected as study objects. Asthma model group (OVA group), asthmatic group (SEA group) exposed to smoke and Clarithromycin treated group (CAM group) after exposure to smoke were established by ovalbumin sensitization. The lung inflammation was detected by histopathology, the expression of HDAC2mRNA and the expression of HDAC2 and GR protein in lung tissue were detected by PCR and Western blot respectively. Results histopathological observation showed that there was airway inflammation in both OVA and SEA groups, in which the ciliates in SEA group were severely damaged, but inflammatory cell infiltration was weakened in CAM group. 涓嶤AM緇勭浉姣，

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