【摘要】：目的：通過分別檢測支氣管哮喘患者急性發(fā)作期外周血中白細(xì)胞介素-25、嗜酸性粒細(xì)胞、白細(xì)胞總數(shù)、中性粒細(xì)胞和肺功能的水平，研究支氣管哮喘患者IL-25及嗜酸性粒細(xì)胞與肺功能的關(guān)系，以及這些指標(biāo)與支氣管哮喘之間的相關(guān)性，進(jìn)而分析這些指標(biāo)在支氣管哮喘臨床診斷中的作用，為支氣管哮喘臨床治療尋找依據(jù)。 方法：收集記錄完整的36例支氣管哮喘患者的個人資料（包括年齡、性別、病程、過敏史、過敏性鼻炎史、用藥史等），檢測外周血中白細(xì)胞總數(shù)、中性粒細(xì)胞數(shù)、嗜酸性粒細(xì)胞數(shù)水平，并且采用酶聯(lián)免疫吸附法（ELISA）法檢測樣本中IL-25的水平。同時選取30名于大連醫(yī)科大學(xué)附屬第二醫(yī)院體檢中心進(jìn)行體檢的非支氣管哮喘體檢者作為對照組，并且除外其他與IL-25相關(guān)的因素，測定其外周血中白細(xì)胞介素-25、嗜酸性粒細(xì)胞、白細(xì)胞總數(shù)、中性粒細(xì)胞的水平。所有哮喘患者均行肺功能檢查，測定其第一秒用力呼氣容積（FEV1）、第一秒用力呼氣容積占預(yù)計(jì)值百分比（FEV1%pre）、第一秒用力呼氣容積占用力肺活量百分比（FEV1/FVC%）、用力呼氣50%肺活量的瞬間流量占預(yù)計(jì)值的百分比（FEF50%pre）、用力呼氣75%肺活量的瞬間流量占預(yù)計(jì)值的百分比（FEF75%pre）、用力呼氣25-75%肺活量的平均流量占預(yù)計(jì)值的百分比（FEF25-75%pre）等值，比較這些肺功能指標(biāo)與白細(xì)胞介素-25、嗜酸性粒細(xì)胞、白細(xì)胞總數(shù)、中性粒細(xì)胞等的相關(guān)性。 結(jié)果：1.支氣管哮喘組血清中IL-25、肺功能的檢測水平及相關(guān)性：①支氣管哮喘組血清中IL-25水平明顯高于正常對照組（P0.05），且亞組間比較，中度和重度哮喘組IL-25水平明顯高于輕度哮喘組，差異有顯著統(tǒng)計(jì)學(xué)意義。②在支氣管哮喘組中，IL-25水平與FEV1、FEV1%pre、FEV1/FVC%、FEF50%pre、FEF75%pre、FEF25-75%pre的水平存在負(fù)相關(guān)。 2、支氣管哮喘組外周血中嗜酸性粒細(xì)胞的水平及與肺功能的相關(guān)性：支氣管哮喘組中，EOS水平與FEV1、FEV1%pre、FEV1/FVC%、FEF50%pre、FEF75%pre、FEF25-75%pre的水平存在負(fù)相關(guān)。同時，在哮喘組各亞組中，中度組與輕度組EOS的水平有顯著統(tǒng)計(jì)學(xué)差異（P0.05），，與重度組有顯著統(tǒng)計(jì)學(xué)差異（P0.05）)。 3.哮喘組患者血清中IL-25水平與嗜酸性粒細(xì)胞的水平呈正相關(guān)，而與白細(xì)胞總數(shù)、中性粒細(xì)胞數(shù)無相關(guān)性。 結(jié)論：1.哮喘組血清中IL-25水平明顯高于正常對照組，且隨著FEV1/FVC%、FEV1、FEV1%pre等大氣道指標(biāo)和FEF50%pre、FEF75%pre、FEF25-75%pre等小氣道指標(biāo)的下降而增加，呈負(fù)相關(guān)性，提示IL-25是同時反映大、小氣道病變的敏感指標(biāo)，可能作為臨床輔助診斷及判斷病情嚴(yán)重程度的指標(biāo)之一。 2.哮喘組血清EOS水平亦隨著FEV1/FVC%、FEV1%pre和FEF50%pre、FEF75%pre、FEF25-75%pre的下降而增加，呈負(fù)相關(guān)性，且IL-25水平與EOS水平呈正相關(guān)，推測IL-25可能是通過誘導(dǎo)EOS聚集來發(fā)揮其致炎作用的。
[Abstract]:Objective: to detect the levels of interleukin-25 (IL-25), eosinophils, total leukocytes, neutrophils and lung function in peripheral blood of patients with acute asthma. To study the relationship between IL-25, eosinophil and pulmonary function in patients with bronchial asthma, and the correlation between these indexes and asthma, and to analyze the role of these indexes in the clinical diagnosis of bronchial asthma. To find the basis for clinical treatment of bronchial asthma. Methods: the data of 36 patients with bronchial asthma (including age, sex, course of disease, allergic history, allergic rhinitis history, medication history, etc.) were collected and recorded. The total number of white blood cells and the number of neutrophils in peripheral blood were measured. Eosinophilic granulocyte number and IL-25 levels in the samples were detected by enzyme-linked immunosorbent assay (ELISA). Thirty patients with non-bronchial asthma were selected as control group and their peripheral blood interleukin-25 (IL-25) and eosinophilic granulocyte (eosinophilic granulocyte) were determined except for other factors related to IL-25. The total number of white blood cells and the level of neutrophils. All asthmatic patients underwent pulmonary function tests, The first second forced expiratory volume (FEV1), the first second forced expiratory volume as a percentage of the predicted value (FEV1%pre), the first second forced expiratory volume occupied vital capacity percentage (FEV1/FVC%), the forced expiratory volume of 50% vital capacity as a percentage of the predicted value was measured. (FEF50%pre), 75% of forced expiratory vital capacity as a percentage of predicted instantaneous flow (FEF75%pre), and 25 to 75% of forced expiratory vital capacity as a percentage of predicted value (FEF25-75%pre) equivalent. To compare the correlation between these pulmonary function indexes and interleukin-25, eosinophil, leukocyte count, neutrophil, etc. The result is 1: 1. The level and correlation of IL-25, pulmonary function in asthma group; the level of IL-25 in asthma group was significantly higher than that in normal control group (P0.05). The IL-25 level in moderate and severe asthma group was significantly higher than that in mild asthma group (P0.05). The level of IL-25 was negatively correlated with the level of FEV1,FEV1%pre,FEV1/FVC%,FEF50%pre,FEF75%pre,FEF25-75%pre in bronchial asthma group. 2. The level of eosinophil in peripheral blood and its correlation with lung function in bronchial asthma group: bronchitis There was a negative correlation between the level of EOS and the level of FEV1,FEV1%pre,FEV1/FVC%,FEF50%pre,FEF75%pre,FEF25-75%pre in tube asthma group. At the same time, the level of EOS in moderate group and mild group was significantly different from that in severe group (P0.05), and there was significant difference between severe group and moderate group (P0.05). 3). There was a positive correlation between serum IL-25 level and eosinophil level in asthmatic patients, but no correlation with total leukocyte count and neutrophil count. Conclusion 1. The level of serum IL-25 in asthma group was significantly higher than that in normal control group, and increased with the decrease of FEV1/FVC%,FEV1,FEV1%pre and FEF50%pre,FEF75%pre,FEF25-75%pre, indicating that IL-25 was a sensitive index reflecting the large and small airway lesions at the same time. It may be used as one of the indicators of clinical assistant diagnosis and judgement of severity of the disease. 2. 2. The serum EOS level in asthma group increased with the decrease of FEV1/FVC%,FEV1%pre and FEF50%pre,FEF75%pre,FEF25-75%pre, and the level of IL-25 was positively correlated with EOS level. It was speculated that IL-25 might play its role in inflammation by inducing EOS aggregation.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R562.25

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 陳欣;白介素-12與支氣管哮喘[J];國外醫(yī)學(xué)(呼吸系統(tǒng)分冊);2002年06期

2 周士霞;王起龍;袁彥麗;康燕;張輝;張春玲;王靜;;哮喘患者誘導(dǎo)痰中TSLP、IL-25水平測定及意義[J];山東醫(yī)藥;2010年03期

3 劉粉;吳金香;趙繼萍;李鴻佳;劉文;畢文祥;董亮;;氣道上皮IL-25促進(jìn)哮喘氣道重塑[J];細(xì)胞與分子免疫學(xué)雜志;2012年06期

4 陳建華;;炎癥性腸病患者血清IL-23、IL-17、IL-25含量及臨床意義[J];中華臨床醫(yī)師雜志(電子版);2012年03期

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