【摘要】：目的:探討抗IL-1β和TNF-αIgY抗體霧化吸入治療豚鼠過敏性支氣管哮喘的機(jī)制。方法:Hartly豚鼠隨機(jī)分為霧化吸入生理鹽水的正常對照組(C組),以及霧化吸入卵清白蛋白(OVA)建立過敏性哮喘模型的哮喘模型組(M組)、1.0%抗IL-1β和TNF-αIgY模型豚鼠治療組(Z1組)和布地奈德混懸液模型豚鼠治療組(Z2組)。各組豚鼠末次治療結(jié)束后2、4、8、24 h心臟穿刺取血;收集支氣管肺泡灌洗液(BALF)。美藍(lán)及伊紅染色計(jì)數(shù)血液嗜酸性粒細(xì)胞、嗜中性粒細(xì)胞、淋巴細(xì)胞、巨噬細(xì)胞、嗜堿性粒細(xì)胞。ELISA法測定血漿和BALF中IL-1β、IL-4、IL-6、IL-8、IL-13、IL-16、TNF-α、TGF-β1、IgE的含量。結(jié)果:Z1組嗜酸性粒細(xì)胞在2~8 h低于M組。Z1組血漿IL-1β(4、24 h)、IL-16(2、4、24 h)、TGF-β1(4~24 h)、IgE(24 h)較M組顯著降低(P0.05),而在BALF中IL-1β和TNF-α(2~8 h),IL-4、IL-6、IL-8和IL-13(4~24 h),IL-16(8 h)、TGF-β1和IgE(4~8 h)顯著降低于M組,尤其是IL-1β和TNF-α在2 h就開始顯著降低(P0.05)。IL-1β及TNF-α與IL-4、IL-6、IL-8、IL-13、IL-16、TGF-β1、IgE等呈正相關(guān)(P0.05)。結(jié)論:抗IL-1β和TNF-αIgY霧化吸入減輕OVA誘導(dǎo)的豚鼠過敏性哮喘炎癥可能是通過降低多種過敏性炎癥因子濃度而實(shí)現(xiàn)。
[Abstract]:Objective: to investigate the mechanism of anti-IL-1 尾 and anti-TNF- 偽 IgY aerosol inhalation in the treatment of allergic bronchial asthma in guinea pigs. Methods Guinea pigs were randomly divided into normal control group (group C) and asthmatic model group (group M) induced by atomized ovalbumin (OVA) (group M), which were treated with 1.0% anti IL-1 尾 and TNF- 偽 IgY (group Z1). Guinea pig model treated with budesonide suspension (group Z 2). After the last treatment in each group, the blood samples were taken from the heart for 24 hours after 2 minutes and 8 minutes. The bronchoalveolar lavage fluid (BALF).) was collected from the guinea pigs with bronchoalveolar lavage fluid. Blood eosinophils, neutrophils, lymphocytes, macrophages and basophil were counted by methylene blue and eosinophil staining. Elisa method was used to detect the content of IL-1 尾 -IL-4, IL-6, IL-8, IL-13, TNF- 偽, TGF- 尾 _ 1 and IgE in plasma and BALF. Results the levels of IL-1 尾 (424 h) and TGF- 尾 1 (424 h) in group Z1 were significantly lower than those in group M (P 0.05), while the levels of IL-1 尾 and TNF- 偽 (2h) in BALF were significantly lower than those in group M (P 0.05). The levels of IL-1 尾 and TNF- 偽 (2h) in BALF and IL-13 (424 h) TGF- 尾 1 and IgE (4h) were significantly lower than those in group M (P 0.05). In particular, IL-1 尾 and TNF- 偽 began to decrease significantly at 2 h (P0.05). IL-1 尾 and TNF- 偽 were positively correlated with IL-4, IL-6, IL-8, IL-13, IL-16, TGF- 尾 1-IgE and so on (P0.05). Conclusion: the anti IL-1 尾 and TNF- 偽 IgY aerosol inhalation may reduce the inflammation of allergic asthma induced by OVA in guinea pigs by reducing the concentration of many kinds of allergic inflammatory factors.
【作者單位】： 南昌大學(xué)醫(yī)學(xué)院;南昌大學(xué)醫(yī)學(xué)院研究院;復(fù)旦大學(xué)附屬中山醫(yī)院放療科;江西省人民醫(yī)院;江西省醫(yī)學(xué)科學(xué)研究院;
【基金】：江西省科技廳社會發(fā)展重大招標(biāo)課題(2006第四批)
【分類號】：R562.25
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本文編號：2182349