發(fā)布時(shí)間：2018-07-15 19:57

【摘要】：目的肺是多器官功能障礙中最容易受累的器官,嚴(yán)重感染、創(chuàng)傷、休克、酸中毒及有害氣體吸入等多種因素均可誘發(fā)急性肺損傷(acute lung injury, ALI),其是臨床上常見(jiàn)的導(dǎo)致危及生命的急性呼吸衰竭原因之一。本研究通過(guò)對(duì)膿毒癥誘導(dǎo)急性肺損傷大鼠模型研究,探討谷氨酰胺對(duì)膿毒癥大鼠急性肺損傷時(shí)的肝細(xì)胞生長(zhǎng)因子(HGF)及角質(zhì)細(xì)胞生長(zhǎng)因子(KGF)的作用,以期進(jìn)一步探討谷氨酰胺對(duì)肺損傷后組織修復(fù)的影響。 方法采用改良盲腸結(jié)扎穿刺法復(fù)制大鼠膿毒癥急性肺損傷模型,40只健康SD大鼠,分為3組,即對(duì)照組、手術(shù)組[改良盲腸結(jié)扎穿刺法]、谷氨酰胺治療組[采用改良盲腸結(jié)扎穿刺法后靜脈注射3%谷氨酰胺0.75g/kg]。實(shí)驗(yàn)中手術(shù)組大鼠死亡6只,治療組大鼠死亡3只,在手術(shù)6h后手術(shù)組隨機(jī)處死6只,24h后處死剩下5只,治療組藥物處理6h后處死6只,24h后處死剩下的8只,留取標(biāo)本行血?dú)夥治?肺干濕重測(cè)量,4000r/min離心10min,收集血清,置于-20℃冰箱保存待用。Elisa法測(cè)定血清中HGF和KGF的含量。并進(jìn)行各組肺組織的病理學(xué)光鏡檢查。研究谷氨酰胺對(duì)急性肺損傷大鼠組織修復(fù)的影響。 結(jié)果 1.HGF和KGF的比較：與對(duì)照組相比較,手術(shù)組及治療組在術(shù)后6小時(shí)、24小時(shí)HGF及KGF含量明顯升高(p0.05),治療組術(shù)后6小時(shí)、24小時(shí)HGF及KGF含量較手術(shù)組明顯升高(p0.05),手術(shù)組術(shù)后6小時(shí)與24小時(shí)相比較,HGF、KGF含量在6h、24h間差異無(wú)顯著性(P0.05)。 2.血?dú)饨Y(jié)果的比較：與對(duì)照組比較,動(dòng)脈氧分壓(partial pressureof oxygen in artery, PaO2)在改良盲腸結(jié)扎穿刺術(shù)后6小時(shí)、24小時(shí)各組均有下降(P0.05)；在術(shù)后6小時(shí)、24小時(shí),手術(shù)組較同時(shí)間點(diǎn)谷氨酰胺治療組下降的差異有統(tǒng)計(jì)學(xué)意義(P0.05)； 3.肺干濕重的比較：實(shí)驗(yàn)組、治療組在術(shù)后6小時(shí)、24小時(shí)的肺濕干重比值與對(duì)照組之間存在增高表現(xiàn)(p0.05),手術(shù)組較治療組在術(shù)后6小時(shí)、24小時(shí)肺濕干重比均明顯增高(P0.05)。 結(jié)論 1.靜脈給予谷氨酰胺能促進(jìn)急性肺損傷大鼠血清中HGF、KGF濃度水平的上升,從而促進(jìn)受損組織的修復(fù)。
[Abstract]:Objective the lung is one of the most easily involved organs in multiple organ dysfunction, with severe infection, trauma and shock. Acidosis and inhalation of harmful gases can induce acute lung injury (acute lung injury, Ali), which is one of the common causes of life-threatening acute respiratory failure. In this study, we investigated the effects of glutamine on hepatocyte growth factor (HGF) and keratinocyte growth factor (KGF) during acute lung injury induced by sepsis in rats. To further explore the effect of glutamine on tissue repair after lung injury. Methods Forty SD rats with septic acute lung injury model were established by modified cecal ligation puncture method and divided into 3 groups: control group. Operation group [modified cecal ligation puncture method], glutamine treatment group [modified cecum ligation puncture method after intravenous injection of 3% glutamine 0.75g/kg]. In the experiment, 6 rats died in the operation group, 3 rats died in the treatment group, 5 rats were killed at random in the operation group after 6 hours of operation, 6 rats in the treatment group were killed after 6 hours of drug treatment, and 8 rats were killed after 24 hours of drug treatment. The samples were collected for blood gas analysis, the lung dry-wet weight was measured by 4000r / min centrifugation for 10 mins, and the serum was collected and stored in -20 鈩，

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