【摘要】：目的肺是多器官功能障礙中最容易受累的器官,嚴重感染、創(chuàng)傷、休克、酸中毒及有害氣體吸入等多種因素均可誘發(fā)急性肺損傷(acute lung injury, ALI),其是臨床上常見的導致危及生命的急性呼吸衰竭原因之一。本研究通過對膿毒癥誘導急性肺損傷大鼠模型研究,探討谷氨酰胺對膿毒癥大鼠急性肺損傷時的肝細胞生長因子(HGF)及角質細胞生長因子(KGF)的作用,以期進一步探討谷氨酰胺對肺損傷后組織修復的影響。 方法采用改良盲腸結扎穿刺法復制大鼠膿毒癥急性肺損傷模型,40只健康SD大鼠,分為3組,即對照組、手術組[改良盲腸結扎穿刺法]、谷氨酰胺治療組[采用改良盲腸結扎穿刺法后靜脈注射3%谷氨酰胺0.75g/kg]。實驗中手術組大鼠死亡6只,治療組大鼠死亡3只,在手術6h后手術組隨機處死6只,24h后處死剩下5只,治療組藥物處理6h后處死6只,24h后處死剩下的8只,留取標本行血氣分析,肺干濕重測量,4000r/min離心10min,收集血清,置于-20℃冰箱保存待用。Elisa法測定血清中HGF和KGF的含量。并進行各組肺組織的病理學光鏡檢查。研究谷氨酰胺對急性肺損傷大鼠組織修復的影響。 結果 1.HGF和KGF的比較：與對照組相比較,手術組及治療組在術后6小時、24小時HGF及KGF含量明顯升高(p0.05),治療組術后6小時、24小時HGF及KGF含量較手術組明顯升高(p0.05),手術組術后6小時與24小時相比較,HGF、KGF含量在6h、24h間差異無顯著性(P0.05)。 2.血氣結果的比較：與對照組比較,動脈氧分壓(partial pressureof oxygen in artery, PaO2)在改良盲腸結扎穿刺術后6小時、24小時各組均有下降(P0.05)；在術后6小時、24小時,手術組較同時間點谷氨酰胺治療組下降的差異有統(tǒng)計學意義(P0.05)； 3.肺干濕重的比較：實驗組、治療組在術后6小時、24小時的肺濕干重比值與對照組之間存在增高表現(xiàn)(p0.05),手術組較治療組在術后6小時、24小時肺濕干重比均明顯增高(P0.05)。 結論 1.靜脈給予谷氨酰胺能促進急性肺損傷大鼠血清中HGF、KGF濃度水平的上升,從而促進受損組織的修復。
[Abstract]:Objective the lung is one of the most easily involved organs in multiple organ dysfunction, with severe infection, trauma and shock. Acidosis and inhalation of harmful gases can induce acute lung injury (acute lung injury, Ali), which is one of the common causes of life-threatening acute respiratory failure. In this study, we investigated the effects of glutamine on hepatocyte growth factor (HGF) and keratinocyte growth factor (KGF) during acute lung injury induced by sepsis in rats. To further explore the effect of glutamine on tissue repair after lung injury. Methods Forty SD rats with septic acute lung injury model were established by modified cecal ligation puncture method and divided into 3 groups: control group. Operation group [modified cecal ligation puncture method], glutamine treatment group [modified cecum ligation puncture method after intravenous injection of 3% glutamine 0.75g/kg]. In the experiment, 6 rats died in the operation group, 3 rats died in the treatment group, 5 rats were killed at random in the operation group after 6 hours of operation, 6 rats in the treatment group were killed after 6 hours of drug treatment, and 8 rats were killed after 24 hours of drug treatment. The samples were collected for blood gas analysis, the lung dry-wet weight was measured by 4000r / min centrifugation for 10 mins, and the serum was collected and stored in -20 鈩，

本文編號：2125211