本文選題：IL-5 + IL-12�。� 參考：《青海大學(xué)》2012年碩士論文

【摘要】：目的本文用ELISA法對不同海拔地區(qū)100例慢性阻塞性肺疾�。–OPD）患者進(jìn)行了血清IL-5、IL-12水平的測定，探討它們在不同海拔COPD發(fā)病機(jī)制中的作用及意義。 方法選擇中海拔（2200m）AECOPD患者37例、緩解期患者31例和對照組30例，分別為中海拔AECOPD組、緩解期組和對照組，高海拔（4500m）COPD緩解期患者32例和對照組32例，分別為高海拔緩解期組和對照組，用ELISA法測定血清IL-5、IL-12水平。 結(jié)果中海拔AECOPD組血清IL-5水平明顯高于緩解期組和對照組，緩解期組血清IL-5水平低于對照組（P＜0.05）；高海拔緩解期組和對照組血清IL-5水平無差異（P＞0.05）；高海拔緩解期組和對照組血清IL-5水平均高于中海拔緩解期組和中海拔對照組（P＜0.05）。中海拔緩解期組血清IL-12水平高于AECOPD組和對照組（P＜0.05）；中海拔AECOPD組血清IL-12水平和對照組無差異（P＞0.05）；高海拔緩解期組血清IL-12水平高于對照組（P＜0.05）；高海拔緩解期組血清IL-12水平低于中海拔緩解期組（P＜0.05）；高海拔對照組和中海拔對照組血清IL-12水平無差異（P＞0.05）。中海拔AECOPD組FEV1與血清IL-5水平呈負(fù)相關(guān)（r=-0.744，，P 0.05），而FEV1與血清IL-12水平呈正相關(guān)（r=0.738，P 0.05）。 結(jié)論1、AECOPD組血清IL-5水平高于緩解期組，血清IL-12水平低于緩解期組，提示血清IL-5參與COPD氣道急性炎癥反應(yīng)，血清IL-12參與COPD氣道損傷修復(fù)；2、高海拔COPD緩解期組和對照組血清IL-5水平高于中海拔緩解期組和對照組，高海拔COPD緩解期組血清IL-12水平低于中海拔COPD緩解期組，提示IL-5、IL-12參與了低氧高寒環(huán)境下COPD低氧適應(yīng)過程；3、AECOPD組血清IL-5水平越高，患者FEV1越低，而血清IL-12則相反，提示IL-5和IL-12參與COPD氣道改變。
[Abstract]:Objective to study the role of IL-5 and IL-12 in the pathogenesis of COPD in 100 patients with chronic obstructive pulmonary disease (COPD) at different elevations by Elisa. Methods Thirty-seven patients with moderate altitude (2200m) AECOPD, 31 patients in remission period and 30 patients in control group were selected as middle altitude AECOPD group, remission group and control group, 32 patients with COPD at high altitude (4500m) and 32 patients with COPD at high altitude (4500m). The serum levels of IL-5 and IL-12 were measured by Elisa in remission stage group and control group. Results the level of serum IL-5 in AECOPD group was significantly higher than that in remission group and control group (P < 0.05), but there was no difference between high altitude group and control group (P > 0.05). The levels of serum IL-5 in the remission period group and control group were higher than those in the middle altitude remission stage group and the middle altitude control group (P < 0.05). The level of serum IL-12 in the remission period group was higher than that in the AECOPD group and the control group (P < 0.05), the serum IL-12 level in the AECOPD group was not different from that in the control group (P > 0.05), the serum IL-12 level in the high altitude remission period group was higher than that in the control group (P < 0.05). The level of serum IL-12 in the remission period group was lower than that in the middle altitude group (P < 0.05), but there was no difference between the high altitude control group and the middle altitude control group (P > 0.05). There was a negative correlation between FEV1 and serum IL-5 level in AECOPD group (r = 0.744, P 0.05), but a positive correlation between FEV _ 1 and serum IL-12 level (r = 0.738, P 0.05). Conclusion 1the level of serum IL-5 in AECOPD group is higher than that in remission group, and the level of serum IL-12 is lower than that in remission group, suggesting that serum IL-5 is involved in acute airway inflammation of COPD, and serum IL-12 is involved in the repair of COPD airway injury. 2, the serum IL-5 level of COPD remission period and control group at high altitude is higher than that of middle altitude remission stage and control group, and the serum IL-12 level in high altitude COPD remission phase is lower than that in middle altitude COPD remission stage group, which suggests that IL-5 IL-12 is involved in the process of COPD hypoxia adaptation under hypoxia and alpine environment. 3 the higher the level of serum IL-5 in AECOPD group, the lower the level of FEV1, whereas the lower the level of serum IL-12, suggesting that IL-5 and IL-12 are involved in the airway changes of COPD.
【學(xué)位授予單位】：青海大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R563.9

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