本文選題：急性肺損傷 + 髓樣細胞表達的觸發(fā)受體��； 參考：《實用醫(yī)學(xué)雜志》2017年22期

【摘要】：目的觀察急性肺損傷(ALI)小鼠肺內(nèi)髓樣細胞表達的觸發(fā)受體-1(TREM-1)與內(nèi)質(zhì)網(wǎng)應(yīng)激的相關(guān)性。方法以Balb/c小鼠為研究對象,氣管注射脂多糖(LPS,5 mg/kg)復(fù)制小鼠ALI模型,采用real-time PCR法檢測肺組織TREM-1與內(nèi)質(zhì)網(wǎng)應(yīng)激相關(guān)蛋白CHOP和GRP78 mRNA的表達,并分析TREM-1與內(nèi)質(zhì)網(wǎng)相關(guān)蛋白基因表達的相關(guān)性。LPS(100 ng/mL)誘導(dǎo)小鼠原代腹腔巨噬細胞炎癥反應(yīng),檢測巨噬細胞TREM-1、CHOP和GRP78 mRNA表達并分析其相關(guān)性。采用TREM-1激活抗體處理小鼠巨噬細胞后,觀察其對CHOP和GRP78表達的影響。結(jié)果 ALI小鼠肺內(nèi)TREM-1 mRNA與內(nèi)質(zhì)網(wǎng)應(yīng)激相關(guān)蛋白CHOP和GRP78 mRNA表達顯著增加,TREM-1與CHOP和GRP78表達呈正相關(guān)。LPS可上調(diào)小鼠巨噬細胞TREM-1、CHOP和GRP78 mRNA的表達,且TREM-1與CHOP和GRP78呈正相關(guān)。激活TREM-1可增加CHOP和GRP78 mRNA表達。結(jié)論 ALI小鼠肺內(nèi)TREM-1和內(nèi)質(zhì)網(wǎng)應(yīng)激呈正相關(guān),激活TREM-1可增強巨噬細胞內(nèi)質(zhì)網(wǎng)應(yīng)激。
[Abstract]:Objective to investigate the relationship between the expression of trigger receptor 1 (TREM-1) and endoplasmic reticulum stress in acute lung injury (Ali) mice. Methods the Ali model of Balb / c mice was induced by endotracheal injection of lipopolysaccharide (LPS5 mg/kg). The expression of TREM-1 and endoplasmic reticulum stress-related proteins chop and GRP78 in lung tissue was detected by real-time PCR. The relationship between TREM-1 and endoplasmic reticulum associated protein gene expression. LPS (100 ng / mL) induced inflammatory reaction of primary peritoneal macrophages in mice. The expression of TREM-1 and GRP78 mRNA in macrophages was detected and the correlation between TREM-1 and GRP78 mRNA was analyzed. The effect of TREM-1 activated antibody on the expression of chop and GRP78 in mouse macrophages was observed. Results the expressions of TREM-1 mRNA and endoplasmic reticulum stress-related proteins chop and GRP78 in lung of Ali mice were significantly increased. The expression of TREM-1 was positively correlated with the expression of chop and GRP78. LPS could up-regulate the expression of TREM-1 and GRP78 mRNA in mouse macrophages, and the expression of TREM-1 was positively correlated with chop and GRP78. Activation of TREM-1 increased the expression of chop and GRP78 mRNA. Conclusion there is a positive correlation between TREM-1 and endoplasmic reticulum stress in Ali mice. Activation of TREM-1 can enhance endoplasmic reticulum stress in macrophages.
【作者單位】： 遵義醫(yī)學(xué)院附屬醫(yī)院麻醉科;
【基金】：國家自然科學(xué)基金(編號:81660021) 遵義醫(yī)學(xué)院大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練計劃項目(編號:20162312)
【分類號】：R563
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本文編號：2051663