本文選題：支氣管哮喘 + 單核甘酸多態(tài)性　； 參考：《吉林大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2014年05期

【摘要】：目的:觀察幾丁質(zhì)酶3類1(CHI3L1)基因多態(tài)性與廣東地區(qū)漢族人群哮喘發(fā)病的關(guān)聯(lián)性,探討采用其編碼的幾丁質(zhì)酶樣蛋白40(YKL-40)評(píng)估病情嚴(yán)重程度及監(jiān)測(cè)病情的臨床意義。方法:入選的251例研究對(duì)象分為哮喘組(150例)和正常對(duì)照組(101例)。采用Massarray法檢測(cè)CHI3L1基因單核苷酸多態(tài)性(SNP),同時(shí)檢測(cè)哮喘組和對(duì)照組研究對(duì)象血清YKL-40水平、外周血嗜酸性粒細(xì)胞百分比(Eos%)、總IgE和肺功能,同時(shí)將哮喘組分為急性加重期和穩(wěn)定期2個(gè)亞組,比較患者血清YKL-40水平的差異。結(jié)果:CHI3L1基因位點(diǎn)rs3806448A/G等位基因在哮喘組和對(duì)照組中分布比較差異有統(tǒng)計(jì)學(xué)意義(P0.01),哮喘組患者rs3806448等位基因A頻數(shù)明顯高于正常對(duì)照組(OR=1.93,95%CI=1.30~2.62,χ2=11.6,P0.01);rs3806448基因型為AA患者血清YKL-40水平、總IgE和Eos%高于基因型為GG或AG患者(P0.05);而rs3806448基因型為AA患者一秒用力呼氣容積(FEV1)占預(yù)計(jì)值百分比(FEV1%)低于基因型為GG或AG患者(P0.05);哮喘組患者血清YKL-40水平明顯高于正常對(duì)照組(P0.01),哮喘急性加重組患者血清YKL-40水平高于病情穩(wěn)定組和正常對(duì)照組(P0.01);哮喘組患者血清YKL-40水平與外周血EOS%及總IgE水平呈正相關(guān)關(guān)系(rEos=0.348,rIgE=0.437,P0.01),與肺功能呈負(fù)相關(guān)關(guān)系(r=-0.745,P0.01)。結(jié)論:CHI3L1-rs3806448多態(tài)性與廣東地區(qū)漢族人群的哮喘發(fā)病有關(guān)聯(lián),提示血清YKL-40可能是評(píng)價(jià)哮喘患者病情嚴(yán)重程度及監(jiān)測(cè)病情變化的一個(gè)新生物標(biāo)志物。
[Abstract]:Objective: to investigate the association between chitinase class 3 (CHI3L1) gene polymorphism and asthma in Guangdong Han population, and to explore the clinical significance of evaluating the severity of asthma by using chitinase-like protein 40 (YKL-40). Methods: 251 subjects were divided into asthma group (n = 150) and normal control group (n = 101). The single nucleotide polymorphisms (SNPs) of CHI3L1 gene were detected by Massarray method. The levels of YKL-40, the percentage of eosinophils, total IgE and lung function were measured in asthmatic and control groups. At the same time, the asthmatic group was divided into two subgroups: acute exacerbation group and stable stage group, and the serum YKL-40 levels were compared. Results there was a significant difference in the distribution of rs3806448A / G allele between asthma group and control group. The frequency of rs3806448 allele A in asthmatic group was significantly higher than that in normal control group (OR1.93 / 95) 1.30 ~ 2.62, 蠂 ~ (211.6P0.01rs3806448) genotype was the serum YKL-40 level of AA patients. Total IgE and Eos% were higher than those in patients with genotype GG or AG (P 0.05), but the percentage of FEV1 in predicted value of patients with rs3806448 genotype AA was lower than that of patients with GG or AG, and the level of YKL-40 in patients with asthma was significantly higher than that in patients with normal controls. The serum levels of YKL-40 in patients with acute exacerbation of asthma were higher than those in patients with stable condition and normal controls, and the levels of EOS% and total IgE in peripheral blood were positively correlated with the levels of EOS% and total IgE in patients with asthma, and were negatively correlated with pulmonary function. Conclusion there is a correlation between the polymorphism of 1: CHI3L1-rs3806448 and the incidence of asthma in Guangdong Han population, suggesting that serum YKL-40 may be a new biomarker for evaluating the severity of asthma and monitoring its changes.
【作者單位】： 暨南大學(xué)附屬第一醫(yī)院呼吸內(nèi)科;廣東醫(yī)學(xué)院附屬醫(yī)院呼吸內(nèi)科;
【基金】：廣東省科技廳科技計(jì)劃項(xiàng)目資助課題(2010B031600285)
【分類號(hào)】：R562.25

【相似文獻(xiàn)】

相關(guān)期刊論文 前1條

1 胡麗蓉;王榮麗;;YKL-40與肺部疾病的關(guān)系[J];中華哮喘雜志(電子版);2010年06期

相關(guān)會(huì)議論文 前1條

1 李U，

本文編號(hào)：1998854