本文選題：計(jì)算機(jī)斷層掃描 + 百草枯��； 參考：《河北北方學(xué)院》2013年碩士論文

【摘要】：建立合適的百草枯致肺損傷的模型,為后續(xù)研究提供必要的條件。健康新西蘭大白兔70只,隨機(jī)分為四組,每小組10只,分別以不同劑量百草枯染毒,第一小組百草枯劑量25mg.kg-1、第二小組劑35mg.kg-1、第三小組劑量45mg.kg-1第四小組劑量50mg.kg-l,確定半數(shù)致死量。分別在預(yù)設(shè)時(shí)間點(diǎn)1h、6h、12h、24h、48h及72h每組抽取3只,開胸取雙肺上、中、下野6處標(biāo)本,分別進(jìn)行(1)HE染色檢查；(2)電鏡超微結(jié)構(gòu)觀察。以百草枯35mg.kg-1劑量選為最適劑量,做為后續(xù)CT灌注對(duì)化學(xué)性肺損傷的穩(wěn)定、可靠的模型。研究320排CT容積灌注對(duì)化學(xué)性肺損傷的早期血流動(dòng)力學(xué)改變?cè)u(píng)價(jià),且與病理學(xué)對(duì)照,以尋求CT灌注(CT Perfusion)在化學(xué)性肺損傷早期功能學(xué)改變的監(jiān)測(cè)及診斷價(jià)值。30只新西蘭大白兔分為對(duì)照組及實(shí)驗(yàn)組(0時(shí)刻為對(duì)照組)。采用百草枯(paraquat,PQ)35mg.kg劑量為最適劑量,于Oh、2h、6h、12h、24h、48h行320排胸部CT灌注掃描,并于上述時(shí)間點(diǎn)空氣栓塞處死4只取肺組織做病理檢測(cè)。(1)對(duì)照組肺的rBF、rBV及rPS平均值分別為128.54士31.31、25.20士4.15、13.06士3.05；(2)實(shí)驗(yàn)組肺CTP參數(shù)變化與病理改變有顯著相關(guān)性。毛細(xì)血管內(nèi)皮細(xì)胞腫脹程度評(píng)級(jí)和血流量呈負(fù)相關(guān),r=-0.848,p=0.033。毛細(xì)血管基底膜破壞程度評(píng)級(jí)和血管通透性呈正相關(guān),r=0.893,p=0.016。百草枯投藥后,隨時(shí)間的推移肺血流量呈逐漸下降趨勢(shì),方差分析提示24小時(shí)血流量較未給藥時(shí)(0時(shí)刻)有顯著下降,p0.001。百草枯投藥后,隨時(shí)間的推移肺血管通透性呈逐漸上升趨勢(shì),方差分析提示2小時(shí)血管通透性較未給藥時(shí)(0時(shí)刻)有顯著變化,p0.001。血漿TGF-β1含量變化與灌注參數(shù)：PS的相關(guān)性檢驗(yàn)結(jié)果:Pearson相關(guān)系r=0.07580,p=0.68520.05,血漿TNF-a含量變化與灌注參數(shù)PS的相關(guān)性檢驗(yàn)結(jié)果:Pearson相關(guān)系r=0.42081,P=0.385620.05。均無(wú)統(tǒng)計(jì)學(xué)差異,說明血漿TGF-β1、TNF-a變化與灌注參數(shù)PS無(wú)顯著相關(guān)性。CTP參數(shù)(尤其是PS)能反映百草枯所致化學(xué)性肺損傷超早期血流動(dòng)力學(xué)異常改變,有望用于早期預(yù)報(bào)及動(dòng)態(tài)監(jiān)測(cè)早期肺損傷的發(fā)生。
[Abstract]:A suitable model of paraquat induced lung injury was established to provide necessary conditions for further study. 70 healthy New Zealand white rabbits were randomly divided into four groups, 10 rabbits in each group. The first group was treated with paraquat 25 mg 路kg -1, the second group 35 mg 路kg -1, the third group 45mg.kg-1 group 4 50 mg 路kg -1, and the second group 50 mg 路kg -1. At the preset time point of 1h, 6h, 12h, 24h, 48h and 72h, respectively, 3 rats were taken from each group, and 6 specimens were collected from the upper, middle and lower lungs respectively. The ultrastructure of the two groups were examined by HE staining. The optimal dose of paraquat 35mg.kg-1 was chosen as a stable and reliable model for the follow-up CT perfusion to chemically induced lung injury. To study the evaluation of early hemodynamic changes in patients with chemical lung injury by volume perfusion with 320 slice CT, and compared with pathology. To explore the value of CT perfusion CT perfusion in monitoring and diagnosis of early functional changes in chemical lung injury. Thirty New Zealand white rabbits were divided into two groups: control group and experimental group. The optimal dose of paraquatine (PQQ) was 35mg 路kg / kg, and 320-slice CT perfusion scan was performed at 2 h ~ 6 h ~ 12 h ~ 24 h ~ 48 h. At the above time point, 4 lung tissues were killed by air embolization for pathological examination.) the mean values of rBF- rBV and rPS in the control group were 128.54 鹵31.31 鹵25.20 鹵4.15 ~ 13.06 鹵3.05 ~ 2 respectively) there was a significant correlation between the changes of lung CTP parameters and pathological changes in the experimental group. There was a negative correlation between capillary endothelial cell swelling and blood flow. There was a positive correlation between the degree of capillary basement membrane damage and vascular permeability. After administration of paraquat, the pulmonary blood flow decreased gradually with the passage of time, and the analysis of variance showed that 24 hours blood flow was significantly lower than that at 0 hour after administration of paraquat (P 0.001). After paraquat administration, the pulmonary vascular permeability increased gradually with time, and the variance analysis showed that the blood vessel permeability at 2 hours was significantly different from that at 0 hour after administration of paraquat (P 0.001). The relationship between the plasma TGF- 尾 1 content and the perfusion parameter: PS. The relationship between the content of TNF-a and the perfusion parameter was 0.68520.05.The results showed that the relationship between the plasma TNF-a content and the perfusion parameter PS was 0.385620.050.385620.05. the relationship between the content of plasma TGF- 尾 _ 1 and the perfusion parameter was 0.385620.05.The results showed that the relationship between plasma TGF- 尾 _ 1 content and perfusion parameter PS was 0.385620.05. There was no statistical difference between the two groups, which indicated that there was no significant correlation between plasma TGF- 尾 1 TNF-a and perfusion parameter PS. CTP parameters (especially PS1) could reflect the abnormal hemodynamic changes in the early stage of chemical lung injury induced by paraquat. It is expected to be used for early prediction and dynamic monitoring of early lung injury.
【學(xué)位授予單位】：河北北方學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R563

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