本文選題：大腸桿菌 + 金黃色葡萄球菌�。� 參考：《浙江大學(xué)》2013年碩士論文

【摘要】：目的： 通過檢測(cè)部分常見致病菌感染的肺組織代謝過程中產(chǎn)生的揮發(fā)性有機(jī)物(volatile organic compounds, VOCs),篩選出各菌株的特征性VOCs,為呼吸氣體檢測(cè)在肺部感染性疾病的早期診斷中的應(yīng)用提供實(shí)驗(yàn)依據(jù)。 方法： 建立致病菌感染的人體外肺組織模型及致病菌感染的動(dòng)物模型,利用固相微萃取聯(lián)合氣相色譜質(zhì)譜儀連用技術(shù)(solid-phase microextraction and gas chromatography mass spectroscopy, SPME/GC-MS),通過對(duì)大腸桿菌、金黃色葡萄球菌及銅綠假單胞菌感染的肺組織所產(chǎn)生的呼吸氣體的檢測(cè),篩選出這三種致病菌的特征性VOCs, 結(jié)果： 在致病菌感染人體外肺組織模型中,大腸桿菌感染產(chǎn)生的VOCs中可以檢測(cè)到十二烷(C12H26)、間-(1,1-二甲基-乙基)-苯(C14H22).十六醇(C16H34O),金黃色葡萄球菌感染產(chǎn)生的VOCs中可以檢測(cè)到C12H26、C14H22、2.5-二(1.1-二甲基-乙基)-苯酚(C14H22O),銅綠假單胞菌感染產(chǎn)生的VOCs中可以檢測(cè)到C12H26、C14H22、2,4,6-三(1,1-二甲基-乙基)-苯酚(C18H30O)。在致病菌感染的動(dòng)物模型(體內(nèi)培養(yǎng)模型)中,大腸桿菌感染產(chǎn)生的VOCs中可以檢測(cè)到特異性標(biāo)志物C16H34O,金黃色葡萄球菌感染產(chǎn)生的VOCs中可以檢測(cè)到特異性標(biāo)志物C14H22O,銅綠假單胞菌感染產(chǎn)生的VOCs中可以檢測(cè)到特異性標(biāo)志物C18H30O。 結(jié)論： 通過兩種感染模型VOCs檢測(cè)結(jié)果可知,大腸桿菌、金黃色葡萄球菌、銅綠假單胞菌感染的肺組織在代謝過程中可產(chǎn)生特征性VOCs,其中十六醇(C16H34O)是大腸桿菌感染肺部組織產(chǎn)生的特征性標(biāo)志物,2,5-二(1,1-二甲基-乙基)-苯酚(C14H22O)是金黃色葡萄球菌感染產(chǎn)生的特征性標(biāo)志物,2,4,6-三(1,1-二甲基-乙基)-苯酚(C18H30O)是銅綠假單胞菌感染產(chǎn)生的特征性標(biāo)志物。
[Abstract]:Purpose :

The volatile organic compounds ( VOCs ) generated during the metabolic process of lung tissues infected by common pathogenic bacteria were detected , and the characteristic VOCs of each strain were screened , and the experimental basis was provided for the application of respiratory gas detection in the early diagnosis of lung infectious diseases .

Method :

By using solid - phase extraction and gas chromatography mass spectroscopy ( GC - MS ) , the characteristic VOCs of these three pathogenic bacteria were selected by means of solid - phase extraction and gas chromatography mass spectroscopy ( GC - MS ) , and the respiratory gases produced by lung tissues infected by E . coli , Staphylococcus aureus and Pseudomonas aeruginosa were selected by solid - phase extraction and gas chromatography mass spectroscopy ( GC - MS ) .

Results :

C12H26 , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O , C14H22O and C14H22O could be detected in VOCs .

Conclusion :

It can be seen from the results of the two kinds of infection models that the lung tissues infected by E . coli , S . aureus and Pseudomonas aeruginosa can produce characteristic VOCs during metabolism , wherein cetyl alcohol ( C16H34O ) is a characteristic marker of E . coli infection of lung tissue , and 2,5 - bis ( 1,1 - dimethyl - ethyl ) - phenol ( C14H22O ) is a characteristic marker of Staphylococcus aureus infection , and 2,4,6 - tris ( 1,1 - dimethyl - ethyl ) - phenol ( C18 H30O ) is a characteristic marker for Pseudomonas aeruginosa infection .
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R563.1

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