本文選題：Shh + Gli　； 參考：《免疫學(xué)雜志》2015年02期

【摘要】：目的探討Sonic hedgehog(SHH)信號通路中Shh、Gli1及核因子-κB(nuclear factor kappa B,NF-κB)在脂多糖誘導(dǎo)的小鼠急性肺損傷中的表達(dá)變化。方法脂多糖腹腔注射復(fù)制小鼠急性肺損傷模型,實(shí)驗(yàn)動物隨機(jī)分為實(shí)驗(yàn)組(脂多糖5 mg/kg)和對照組。分別于給藥后6、12、24 h取肺組織,觀察肺組織病理改變、肺濕干重比值(W/D)、RT-PCR和Western blot檢測Shh、Gli1的m RNA和蛋白表達(dá),Western blot檢測NF-κB的蛋白表達(dá)。結(jié)果實(shí)驗(yàn)組肺組織病理損傷評分及W/D比值明顯高于對照組(P0.05)。Shh的m RNA表達(dá)水平在各時間點(diǎn)均高于對照組(P0.05),且與時間呈正相關(guān)(P0.01)。6 h時Gli1的m RNA表達(dá)水平與對照組無明顯差異(P0.05),12、24 h時明顯高于對照組(P0.01),且與時間呈正相關(guān)(P0.01)。與對照組相比,實(shí)驗(yàn)組6、12、24 h時Shh、Gli1的蛋白表達(dá)水平均明顯升高(P0.05),且與時間呈正相關(guān)(P0.01)。NF-κB的蛋白表達(dá)在各時間點(diǎn)均明顯高于對照組(P0.05)。結(jié)論脂多糖誘導(dǎo)的小鼠急性肺損傷中Shh、Gli1表達(dá)上調(diào)可能是脂多糖通過調(diào)控NF-κB來實(shí)現(xiàn)的。
[Abstract]:Objective to investigate the expression of Sonic hedgehog Sonic Gli1 and NF- 魏 B(nuclear factor kappa BnNF- 魏 B in lipopolysaccharide-induced acute lung injury in mice. Methods the acute lung injury model of mice was induced by intraperitoneal injection of lipopolysaccharide. The experimental animals were randomly divided into two groups: the experimental group (5 mg / kg LPS) and the control group. Lung tissue samples were taken at 6h, 12h, 24 h after administration. Lung pathological changes, lung wet / dry weight ratio (WR) and Western blot were detected for m RNA and protein expression. Western blot was used to detect the protein expression of NF- 魏 B. Results the expression of m RNA in lung tissue of experimental group was significantly higher than that of control group at all time points, and the expression level of m RNA in Gli1 was significantly higher than that in control group at all time points. There was a positive correlation between the expression level of m RNA of Gli1 and that of control group at P0.01.6 h. At 24 h after 12h, the significant difference was significantly higher than that of the control group (P 0.01), and there was a positive correlation with the time. Compared with the control group, the protein expression of Shhfen Gli1 in the experimental group was significantly higher than that in the control group at 24 h, and the expression of the protein was significantly higher than that of the control group at all time points. Conclusion the up-regulation of Shhll-Gli1 expression in lipopolysaccharide induced acute lung injury in mice may be due to the regulation of NF- 魏 B by lipopolysaccharide.
【作者單位】： Department
【基金】：山東省醫(yī)藥衛(wèi)生科技發(fā)展計(jì)劃(2011HZ066)
【分類號】：R563.8

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相關(guān)期刊論文 前3條

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本文編號：1929729