本文選題：慢性阻塞性肺疾病 + 股四頭肌萎縮��； 參考：《中南大學(xué)》2013年碩士論文

【摘要】：目的：TWEAK在COPD大鼠股四頭肌萎縮中的作用機(jī)制及可能的信號(hào)轉(zhuǎn)導(dǎo)通路。 方法：8周齡50只Wistar大鼠,按隨機(jī)法分對(duì)照組20只、COPD模型組30只,COPD模型組采用煙熏+氣管內(nèi)滴注豬胰彈性蛋白酶方法建立COPD模型(共三個(gè)月)；模型評(píng)估：COPD模型組以HE染色觀察肺和氣道組織病理變化、肺功能及大鼠表現(xiàn)判斷造模是否成功。分別使用免疫組化法及Western blot法測(cè)定股四頭肌中NF-κB、 MuRF1和TWEAK表達(dá)并分析其相關(guān)性；所有數(shù)據(jù)使用SPSS18.0軟件進(jìn)行分析。 結(jié)果： 1.COPD模型組大鼠造模成功,肺功能測(cè)定及肺組織病理結(jié)果顯示均符合COPD診斷標(biāo)準(zhǔn)。 2.COPD模型組大鼠體重以及一側(cè)股四頭肌的重量較正常對(duì)照組均有明顯減輕(P0.05,P0.05)。 3.COPD模型組大鼠股四頭肌中TWEAK的表達(dá)明顯高于正常對(duì)照組大鼠(P0.05)。 4. COPD模型組大鼠股四頭肌中NF-κB以及MuRF1的表達(dá)明顯高于對(duì)照組大鼠(P0.05,P0.05) 5.各組大鼠股四頭肌NF-κB、MuRF1的表達(dá)與TWEAK的表達(dá)相關(guān)性分析呈正相關(guān)(P0.05,P0.05) 6.各組大鼠股四頭肌中MuRF1與NF-κB的表達(dá)相關(guān)性分析呈正相關(guān)(P0.05)。 結(jié)論： 1.COPD模型組大鼠股四頭肌中TWEAK表達(dá)明顯增加,且與大鼠股四頭肌萎縮有著密切的關(guān)系。 2.泛素-蛋白酶體途徑可能參與COPD大鼠股四頭肌萎縮。 3. TWEAK引起COPD大鼠股四頭肌萎縮可能與泛素-蛋白酶體途徑的激活相關(guān),且NF-κB可能是其中一個(gè)重要的調(diào)節(jié)因子。含圖22幅,表11個(gè),參考文獻(xiàn)46篇。
[Abstract]:Objective to investigate the mechanism and the possible signal transduction pathway of TWEAK on quadriceps femoris atrophy in COPD rats. Methods 50 Wistar rats at the age of 8 weeks, The COPD model was established by injecting porcine pancreatic elastase into smoke and trachea (3 months), and the pathological changes of lung and airway tissues were observed by HE staining in the model group. Lung function and rat performance were used to determine whether the model was successful or not. The expressions of NF- 魏 B, MuRF1 and TWEAK in quadriceps femoris were detected by immunohistochemical method and Western blot method, respectively. All the data were analyzed by SPSS18.0 software. Results: In 1.COPD model group, the model was successfully established, and the pulmonary function test and pathological results of lung tissue were in accordance with the diagnostic criteria of COPD. The body weight and the weight of quadriceps femoris in 2.COPD group were significantly decreased compared with the control group. The expression of TWEAK in quadriceps femoris in 3.COPD group was significantly higher than that in normal control group (P 0.05). 4. The expression of NF- 魏 B and MuRF1 in quadriceps femoris in COPD group was significantly higher than that in control group. 5. Correlation analysis between NF- 魏 BnMuRF1 expression and TWEAK expression in quadriceps femoris of rats in each group 6. Correlation analysis of MuRF1 and NF- 魏 B expression in quadriceps femoris of rats in each group (P 0.05). Conclusion: The expression of TWEAK in quadriceps femoris in 1.COPD model group was significantly increased and was closely related to quadriceps femoris atrophy. 2. The ubiquitin-proteasome pathway may be involved in quadriceps femoris atrophy in COPD rats. 3. TWEAK induced quadriceps femoris atrophy in COPD rats may be related to the activation of ubiquitin proteasome pathway, and NF- 魏 B may be one of the important regulatory factors. There are 22 figures, 11 tables and 46 references.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R563.9

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