本文選題：慢性阻塞性肺疾病 + NOD2受體　； 參考：《川北醫(yī)學(xué)院》2015年碩士論文

【摘要】：目的:本研究通過對COPD患者外周血單個核細胞核內(nèi)NOD2m RNA的表達及血清中IL-6濃度的測定,根據(jù)對不同分組患者外周血單個核細胞核內(nèi)NOD2 m RNA的表達及血清中IL-6濃度與肺功能的相關(guān)性進行研究,探討NOD2在COPD患者炎癥機制中的作用及臨床意義。方法:川北醫(yī)學(xué)院附屬醫(yī)院呼吸內(nèi)科2013年10月至2014年4月門診及住院緩解后的COPD患者為研究對象。根據(jù)其肺功能(FEV1/FVC%、FEV1占預(yù)計值%)、癥狀(m MRC呼吸困難指數(shù))、過去1年急性加重次數(shù)進行綜合評估,將患者分為A、B、C、D四組,每組30人。同期門診健康體檢者30例作為健康對照組。收集各組患者及對照組外周血標本,用實時熒光定量聚合酶鏈反應(yīng)(PCR)測定標本外周血單個核細胞核內(nèi)NOD2 m RNA的表達,用ELISA法測定標本中的IL-6的濃度。對比分析不同分組COPD患者NOD2 m RNA表達和IL-6濃度的變化,探討COPD患者NOD2 m RNA表達和IL-6的濃度與COPD分組嚴重程度的關(guān)系及二者的相關(guān)性。結(jié)果①COPD穩(wěn)定期組患者及健康對照組的外周血單個核細胞核內(nèi)NOD2 m RNA分別為0.31±0.18、0.10±0.07,COPD穩(wěn)定期組明顯高于健康對照組,差異有統(tǒng)計學(xué)意義(P0.01);COPD穩(wěn)定期患者及健康對照組的血清IL-6的濃度分別為10.62±5.01pg/ml、3.00±0.40pg/ml,COPD穩(wěn)定期組明顯高于健康對照組,差異有統(tǒng)計學(xué)意義(P0.01)。②COPD患者A、B、C、D各組及健康對照組中外周血單個核細胞核內(nèi)NOD2 m RNA分別為0.18±0.10、0.18±0.08、0.36±0.11、0.51±0.17、0.10±0.07,A、B、C、D組與健康對照組比較,差異均具有統(tǒng)計學(xué)意義(P0.01)。COPD患者A、B、C、D各組之間兩兩比較,D組NOD2 m RNA表達明顯高于C組,差異有統(tǒng)計學(xué)意義(P0.01),C組NOD2 m RNA表達明顯高于A組和B組,差異有統(tǒng)計學(xué)意義(P0.01),A組和B組之間差異無統(tǒng)計學(xué)意義(P0.05)。③COPD患者A、B、C、D各組及健康對照組血清IL-6的濃度分別為6.36±1.82pg/ml、6.52±1.97pg/ml、12.12±1.78pg/ml、17.50±2.22pg/ml、3.00±0.40pg/ml,A、B、C、D組與健康對照組比較,差異均具有統(tǒng)計學(xué)意義(P0.01)。COPD患者A、B、C、D各組之間兩兩比較,D組IL-6濃度表達明顯高于C組,差異有統(tǒng)計學(xué)意義(P0.01),C組IL-6濃度明顯高于A組和B組,差異有統(tǒng)計學(xué)意義(P0.01),A組和B組之間差異無統(tǒng)計學(xué)意義(P0.05)。④COPD各組患者外周血單個核細胞核內(nèi)NOD2 m RNA表達隨血清IL-6濃度增加而增加,呈正相關(guān),相關(guān)系數(shù)r=0.82,相關(guān)性有統(tǒng)計學(xué)意義(P0.01)。COPD穩(wěn)定期患者NOD2 m RNA表達與FEV1占預(yù)計值%呈負相關(guān),相關(guān)系數(shù)r=-0.85,相關(guān)性有統(tǒng)計學(xué)意義(P0.01)。COPD穩(wěn)定期患者IL-6濃度與FEV1占預(yù)計值%呈負相關(guān),相關(guān)系數(shù)r=-0.74,相關(guān)性有統(tǒng)計學(xué)意義(P0.01)。結(jié)論①COPD患者NOD2受體NOD2 m RNA表達與IL-6濃度較健康對照組明顯增高,推測NOD2與IL-6參與了COPD的發(fā)生、發(fā)展。②隨著COPD病情加重,NOD2 m RNA表達與IL-6濃度逐漸升高,NOD2 m RNA與IL-6可能成為COPD病情嚴重程度評估的指標。③COPD各組中NOD2 m RNA表達與IL-6濃度呈正相關(guān),兩者可能相互作用促進炎癥反應(yīng)。
[Abstract]:Objective: To investigate the expression of NOD2m RNA in the nucleus of peripheral blood mononuclear in COPD patients and the determination of IL-6 concentration in serum, and to explore the role of NOD2 in the inflammatory mechanism of COPD patients according to the expression of NOD2 m RNA in the peripheral mononuclear nuclei of peripheral blood and the correlation between the serum IL-6 concentration and the pulmonary function in the peripheral blood mononuclear nuclei of different groups of patients. Methods: the COPD patients in the Department of respiratory medicine of Affiliated Hospital of Chuanbei Medical College from October 2013 to April 2014 were studied. According to the pulmonary function (FEV1/FVC%, FEV1 accounted for estimated%), the symptoms (m MRC respiratory difficulty index), the acute aggravation times of the past 1 years were evaluated comprehensively, and the patients were divided into groups of A, B, C, D four groups. 30 people in each group. 30 cases of health check in the same period were used as the healthy control group. The peripheral blood samples of each group and the control group were collected. The expression of NOD2 m RNA in the nucleus of peripheral blood mononuclear cells was measured by real-time fluorescence quantitative polymerase chain reaction (PCR), and the concentration of IL-6 in the specimens was measured by ELISA. The NOD2 of different groups of COPD patients was compared and analyzed. The expression of M RNA and the change of IL-6 concentration were used to investigate the relationship between the NOD2 m RNA expression and the concentration of IL-6 and the severity of COPD group and the correlation between the two. Results (1) the NOD2 m in the peripheral blood mononuclear nuclei of the COPD stable group and the healthy control group was 0.31 + 0.07, respectively, and the stability group was significantly higher than that of the healthy pair. The difference was statistically significant (P0.01). The concentration of serum IL-6 in patients with COPD stable period and healthy control group was 10.62 + 5.01pg/ml, 3 + 0.40pg/ml, and COPD stable period was significantly higher than that in healthy control group (P0.01). (P0.01), A, B, C, D groups and healthy controls. RNA was 0.18 + 0.10,0.18 + 0.08,0.36 + 0.11,0.51 + 0.17,0.10 + 0.07 respectively, A, B, C, and D group compared with the healthy control group, the difference was significantly higher than that between the 22 groups. There was no statistical significance (P0.01), and there was no significant difference between group A and B group (P0.05). (3) the serum IL-6 concentration of A, B, C, D and healthy control group was 6.36 + 1.82pg/ml, 6.52 + 1.97pg/ml, 12.12 +, 17.50 +, 3 +. 0.01) compared with A, B, C, D, the expression of IL-6 concentration in D group was significantly higher than that of C group, and the difference was statistically significant (P0.01). The IL-6 concentration in the C group was significantly higher than that of the A group and the group, the difference was statistically significant. There was a positive correlation with the increase of serum IL-6 concentration, and the correlation coefficient was r=0.82, the correlation was statistically significant (P0.01), the expression of NOD2 m RNA in.COPD stable patients was negatively correlated with the estimated value of FEV1, the correlation coefficient was r=-0.85, and the correlation was statistically significant (P0.01).COPD regular patients were negatively correlated with the expected value%. The correlation was statistically significant (P0.01). Conclusion (1) the NOD2 m RNA expression of NOD2 receptor in COPD patients and IL-6 concentration was significantly higher than that of the healthy control group. It was suggested that NOD2 and IL-6 participated in the COPD occurrence and development. There was a positive correlation between the expression of NOD2 m RNA and the IL-6 concentration in COPD groups, which may interact to promote inflammatory response.
【學(xué)位授予單位】：川北醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R563.9

【參考文獻】

相關(guān)碩士學(xué)位論文 前1條

1 李新勝;慢性阻塞性肺疾病患者血漿可溶性髓樣細胞觸發(fā)性受體-1、白三烯B4測定及臨床意義[D];川北醫(yī)學(xué)院;2014年

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本文編號：1922853