本文選題：矽肺 + 動物模型��； 參考：《中國職業(yè)醫(yī)學》2017年03期

【摘要】：目的探討采用小動物正電子發(fā)射計算機斷層顯像(PET)-計算機體層掃描(CT)動態(tài)觀察活體大鼠矽肺模型的可行性。方法無特定病原體級SD大鼠分為模型組和對照組,模型組一次性氣管內(nèi)注入質(zhì)量濃度為30 g/L的矽塵混懸液建立大鼠矽肺模型,對照組予等體積的0.9%氯化鈉溶液。2組大鼠均于造模后第1、2、3、4、6、8和12周分別隨機選取6只,以小動物PET-CT進行肺部CT檢查并檢測CT值,同時進行PET檢查并檢測氟-18-氟代脫氧葡萄糖標準化攝取值(SUV)。掃描后處死大鼠,檢測肺臟臟器系數(shù),進行肺臟病理組織學觀察,檢測血清轉(zhuǎn)化生長因子β1(TGF-β1)、白細胞介素1(IL-1)和肺組織羥脯氨酸(HYP)水平。結果模型組大鼠肺臟病理組織學改變在早期表現(xiàn)為肺組織炎癥性滲出,隨著時間延長,炎癥反應逐漸減輕,而肺間質(zhì)纖維化逐漸明顯;PET-CT影像學表現(xiàn)與病理組織學改變基本一致。模型組大鼠肺部SUV在第1~3周3個時間點均高于同時間點對照組(P0.05),且在第1~4周隨染塵時間的增加而下降(P0.05)。模型組大鼠肺部CT值在7個時間點均高于同時間點對照組(P0.05),且在第1~6周隨染塵時間的增加而下降(P0.05),在第6~12周隨染塵時間的增加而升高(P0.05)。模型組大鼠肺臟臟器系數(shù)和肺組織HYP水平在7個時間點均高于同時間點對照組(P0.05),且在第1~4周肺臟臟器系數(shù)隨染塵時間的增加而下降(P0.05),在第6~12周肺組織HYP水平隨染塵時間的增加而升高(P0.05)。除第3和4周外,模型組大鼠血清TGF-β1水平在其余5個時間點均高于同時間點對照組(P0.05),且在第1~4周隨染塵時間的增加而下降(P0.05),在第4~8周隨染塵時間的增加而升高(P0.05);模型組大鼠血清IL-1水平在第1~4周4個時間點均高于同時間點對照組(P0.05),且隨染塵時間的增加而下降(P0.05)。結論小動物PET-CT可有效觀察活體矽肺模型大鼠肺部早期炎癥和晚期纖維化,可作為動態(tài)追蹤矽肺大鼠模型肺部損傷的檢測方法。
[Abstract]:Objective to investigate the feasibility of using positron emission computed tomography (PET) in small animals to dynamically observe the model of silicosis in vivo in rats. Methods Sprague-Dawley rats with no specific pathogens were divided into two groups: model group and control group. In the model group, the silicosis model was established by injecting 30 g / L silica dust suspension into the trachea of the model group. The control group was treated with 0.9% sodium chloride solution of equal volume. 6 rats were randomly selected at 8 and 12 weeks after modeling. Lung CT was performed with small animal PET-CT and CT values were measured. At the same time, PET was performed to detect the standardized uptake of fluorine-18-fluorodeoxyglucose (F-18-fluorodeoxyglucose). After scanning, the rats were killed, the lung organ coefficient was measured, the histopathology of lung was observed, the serum levels of transforming growth factor 尾 1 (TGF- 尾 1), interleukin 1 (IL 1) and hydroxyproline (HYP) in lung tissue were detected. Results the pulmonary histopathological changes in the model group showed inflammatory exudation of lung tissue at the early stage, and the inflammatory reaction gradually decreased with the prolongation of time, while the pulmonary interstitial fibrosis gradually showed obvious PET-CT imaging findings consistent with the histopathological changes. The pulmonary SUV in the model group was significantly higher than that in the control group at three time points at the 1st week, and decreased with the increase of the dust exposure time at the 1st and 4th week. The lung CT value in the model group was higher than that in the control group at 7 time points, and decreased with the increase of dust exposure time at the 1st week, and increased with the increase of dust exposure time at the 6th week. The lung organ coefficient and HYP level of lung tissue in the model group were higher than those in the control group at 7 time points, and the lung organ coefficient decreased with the increase of dust exposure time in the 1st week and the lung tissue HYP level in the 6th week and the 12th week in the model group, and the lung tissue HYP level in the model group was higher than that in the control group at the same time point (P 0.05), and the lung organ coefficient decreased with the increase of dust exposure time at the 1st week. With the increase of dust time, P0. 05% was increased. Except for weeks 3 and 4, The serum TGF- 尾 1 level in the model group was higher than that in the control group at the other five time points, and decreased with the increase of dust exposure time at the 1st week, and increased with the dust exposure time at the 48th week, while the serum IL-1 level in the model group was higher than that in the control group. The four time points of the first week were higher than that of the control group at the same time point (P 0.05), and decreased with the increase of the time of dust exposure. Conclusion small animal PET-CT can effectively observe early inflammation and late fibrosis in lung of rats with silicosis in vivo, and can be used as a method to detect lung injury in silicosis model.
【作者單位】： 廣東省職業(yè)病防治院廣東省職業(yè)病防治重點實驗室;貴州醫(yī)科大學;廣東省婦幼保健院;
【基金】：國家科技支撐計劃項目(2014BAI12B01) 國家自然科學基金(81302396) 國家臨床重點專科建設項目(2011-09) 廣東省科技計劃項目(2017A020215159) 廣東省醫(yī)學科研基金(B2016056) 廣東省職業(yè)病防治重點實驗室(2012A061400007)
【分類號】：R135.2;R-332

【相似文獻】

相關期刊論文 前10條

1 劉煥義,周克,魏東,江澤琴,許濤,周進軍;PET-CT在腫瘤臨床中的應用[J];西南軍醫(yī);2005年02期

2 周誠忠;夏海波;夏炎春;宋永浩;李定旺;;PET-CT模擬定位下三維適形放射治療Ⅲ期非小細胞肺癌的療效觀察[J];臨床肺科雜志;2013年11期

3 婁小萍;;PET-CT檢查中應注意的護理環(huán)節(jié)[J];護理實踐與研究;2008年14期

4 王倩;魏旭東;尹青松;周健;劉艷艷;宋永平;;PET-CT在繼發(fā)性噬血細胞綜合征診斷中的應用[J];河南醫(yī)學研究;2011年04期

5 梁穎;吳寧;;PET-CT在評價淋巴瘤療效中的作用及進展[J];癌癥進展;2012年04期

6 華逢春,管一暉,趙軍;~(18)F-氟代脫氧葡萄糖PET及PET-CT在淋巴瘤中的臨床應用[J];國外醫(yī)學(放射醫(yī)學核醫(yī)學分冊);2005年05期

7 盧曉林;劉銳;孫方方;文建民;;PET-CT顯像在口腔頜面部惡性腫瘤患者頸淋巴結轉(zhuǎn)移診斷中的價值[J];江蘇醫(yī)藥;2014年08期

8 趙健,王莉,劉理慧,黃葵紅,黃倩;PET-CT檢查中的護理[J];山東醫(yī)藥;2003年35期

9 黃倩,王莉,黃葵紅;腦部PET-CT檢查55例配合體會[J];山東醫(yī)藥;2005年26期

10 李九林;;PET-CT檢查中的護理配合[J];中國臨床保健雜志;2008年02期

相關會議論文 前10條

1 張爽;孫海波;呂紅;金偉;;正電子發(fā)射計算機斷層顯像(PET-CT)在臨床耳鼻咽喉-頭頸外科的應用[A];中華中醫(yī)藥學會耳鼻喉專業(yè)委員會山東中西醫(yī)結合學會耳鼻喉專業(yè)委員會學術研討會論文匯編[C];2007年

2 王中堂;李寶生;付正;張自成;孫洪福;;PET-CT在Ⅲ期非小細胞肺癌靶區(qū)勾畫中的應用研究[A];2007第六屆全國放射腫瘤學學術年會論文集[C];2007年

3 張聯(lián)合;;PET-CT診斷肝臟孤立性壞死結節(jié)1例[A];首屆浙江省青年核醫(yī)學與分子影像論壇暨浙江省第十三屆核醫(yī)學與放射醫(yī)學防護學術會議論文匯編[C];2013年

4 趙漢璽;;PET-CT對局部晚期非小細胞肺癌三維適形放療影響的劑量學研究[A];2007第六屆全國放射腫瘤學學術年會論文集[C];2007年

5 張霞;陳忠華;歐陽學農(nóng);陳樟樹;;血清腫瘤抗原檢測聯(lián)合CT-掃描或PET-CT對肺癌診斷的比較[A];2010’全國腫瘤分子標志及應用學術研討會暨第五屆中國中青年腫瘤專家論壇論文匯編[C];2010年

6 唐玲瓏;馬駿;陳勇;宗并鳳;孫穎;王巖;吳湖炳;崔念基;;MRI、CT和PET-CT對鼻咽癌咽后淋巴結轉(zhuǎn)移診斷價值的研究[A];2007第六屆全國放射腫瘤學學術年會論文集[C];2007年

7 吳寧;;PET-CT在乳腺癌診治中的應用[A];第二屆全國乳腺影像診斷與技術應用研討會暨學習班論文匯編[C];2007年

8 李文;;腫瘤PET-CT影像[A];2012年華東地區(qū)六省一市核醫(yī)學學術會議論文集[C];2012年

9 李軼忻;;針刺足三里穴-PET-CT腦功能成像的實驗研究[A];第八次全國中西醫(yī)結合影像學術交流大會暨全國中西醫(yī)結合影像學研究進展學習班論文集[C];2005年

10 崔勇;周存升;姚樹展;邵廣瑞;;PET-CT在肺部病變中的臨床應用價值[A];第八次全國中西醫(yī)結合影像學術交流大會暨全國中西醫(yī)結合影像學研究進展學習班論文集[C];2005年

相關碩士學位論文 前2條

1 杜佳輝;超聲內(nèi)鏡、增強CT及PET-CT聯(lián)合應用在食管癌術前分期的應用探討[D];鄭州大學;2012年

2 賈敬好;PET-CT圖像融合在食管癌臨床分期和精確放療中的應用探討[D];河北醫(yī)科大學;2009年

，

本文編號：1919964