發(fā)布時(shí)間：2018-05-21 16:37

  本文選題：矽肺 + 動(dòng)物模型�。� 參考：《中國(guó)職業(yè)醫(yī)學(xué)》2017年03期

【摘要】：目的探討采用小動(dòng)物正電子發(fā)射計(jì)算機(jī)斷層顯像(PET)-計(jì)算機(jī)體層掃描(CT)動(dòng)態(tài)觀察活體大鼠矽肺模型的可行性。方法無(wú)特定病原體級(jí)SD大鼠分為模型組和對(duì)照組,模型組一次性氣管內(nèi)注入質(zhì)量濃度為30 g/L的矽塵混懸液建立大鼠矽肺模型,對(duì)照組予等體積的0.9%氯化鈉溶液。2組大鼠均于造模后第1、2、3、4、6、8和12周分別隨機(jī)選取6只,以小動(dòng)物PET-CT進(jìn)行肺部CT檢查并檢測(cè)CT值,同時(shí)進(jìn)行PET檢查并檢測(cè)氟-18-氟代脫氧葡萄糖標(biāo)準(zhǔn)化攝取值(SUV)。掃描后處死大鼠,檢測(cè)肺臟臟器系數(shù),進(jìn)行肺臟病理組織學(xué)觀察,檢測(cè)血清轉(zhuǎn)化生長(zhǎng)因子β1(TGF-β1)、白細(xì)胞介素1(IL-1)和肺組織羥脯氨酸(HYP)水平。結(jié)果模型組大鼠肺臟病理組織學(xué)改變?cè)谠缙诒憩F(xiàn)為肺組織炎癥性滲出,隨著時(shí)間延長(zhǎng),炎癥反應(yīng)逐漸減輕,而肺間質(zhì)纖維化逐漸明顯;PET-CT影像學(xué)表現(xiàn)與病理組織學(xué)改變基本一致。模型組大鼠肺部SUV在第1~3周3個(gè)時(shí)間點(diǎn)均高于同時(shí)間點(diǎn)對(duì)照組(P0.05),且在第1~4周隨染塵時(shí)間的增加而下降(P0.05)。模型組大鼠肺部CT值在7個(gè)時(shí)間點(diǎn)均高于同時(shí)間點(diǎn)對(duì)照組(P0.05),且在第1~6周隨染塵時(shí)間的增加而下降(P0.05),在第6~12周隨染塵時(shí)間的增加而升高(P0.05)。模型組大鼠肺臟臟器系數(shù)和肺組織HYP水平在7個(gè)時(shí)間點(diǎn)均高于同時(shí)間點(diǎn)對(duì)照組(P0.05),且在第1~4周肺臟臟器系數(shù)隨染塵時(shí)間的增加而下降(P0.05),在第6~12周肺組織HYP水平隨染塵時(shí)間的增加而升高(P0.05)。除第3和4周外,模型組大鼠血清TGF-β1水平在其余5個(gè)時(shí)間點(diǎn)均高于同時(shí)間點(diǎn)對(duì)照組(P0.05),且在第1~4周隨染塵時(shí)間的增加而下降(P0.05),在第4~8周隨染塵時(shí)間的增加而升高(P0.05);模型組大鼠血清IL-1水平在第1~4周4個(gè)時(shí)間點(diǎn)均高于同時(shí)間點(diǎn)對(duì)照組(P0.05),且隨染塵時(shí)間的增加而下降(P0.05)。結(jié)論小動(dòng)物PET-CT可有效觀察活體矽肺模型大鼠肺部早期炎癥和晚期纖維化,可作為動(dòng)態(tài)追蹤矽肺大鼠模型肺部損傷的檢測(cè)方法。
[Abstract]:Objective to investigate the feasibility of using positron emission computed tomography (PET) in small animals to dynamically observe the model of silicosis in vivo in rats. Methods Sprague-Dawley rats with no specific pathogens were divided into two groups: model group and control group. In the model group, the silicosis model was established by injecting 30 g / L silica dust suspension into the trachea of the model group. The control group was treated with 0.9% sodium chloride solution of equal volume. 6 rats were randomly selected at 8 and 12 weeks after modeling. Lung CT was performed with small animal PET-CT and CT values were measured. At the same time, PET was performed to detect the standardized uptake of fluorine-18-fluorodeoxyglucose (F-18-fluorodeoxyglucose). After scanning, the rats were killed, the lung organ coefficient was measured, the histopathology of lung was observed, the serum levels of transforming growth factor 尾 1 (TGF- 尾 1), interleukin 1 (IL 1) and hydroxyproline (HYP) in lung tissue were detected. Results the pulmonary histopathological changes in the model group showed inflammatory exudation of lung tissue at the early stage, and the inflammatory reaction gradually decreased with the prolongation of time, while the pulmonary interstitial fibrosis gradually showed obvious PET-CT imaging findings consistent with the histopathological changes. The pulmonary SUV in the model group was significantly higher than that in the control group at three time points at the 1st week, and decreased with the increase of the dust exposure time at the 1st and 4th week. The lung CT value in the model group was higher than that in the control group at 7 time points, and decreased with the increase of dust exposure time at the 1st week, and increased with the increase of dust exposure time at the 6th week. The lung organ coefficient and HYP level of lung tissue in the model group were higher than those in the control group at 7 time points, and the lung organ coefficient decreased with the increase of dust exposure time in the 1st week and the lung tissue HYP level in the 6th week and the 12th week in the model group, and the lung tissue HYP level in the model group was higher than that in the control group at the same time point (P 0.05), and the lung organ coefficient decreased with the increase of dust exposure time at the 1st week. With the increase of dust time, P0. 05% was increased. Except for weeks 3 and 4, The serum TGF- 尾 1 level in the model group was higher than that in the control group at the other five time points, and decreased with the increase of dust exposure time at the 1st week, and increased with the dust exposure time at the 48th week, while the serum IL-1 level in the model group was higher than that in the control group. The four time points of the first week were higher than that of the control group at the same time point (P 0.05), and decreased with the increase of the time of dust exposure. Conclusion small animal PET-CT can effectively observe early inflammation and late fibrosis in lung of rats with silicosis in vivo, and can be used as a method to detect lung injury in silicosis model.
【作者單位】： 廣東省職業(yè)病防治院廣東省職業(yè)病防治重點(diǎn)實(shí)驗(yàn)室;貴州醫(yī)科大學(xué);廣東省婦幼保健院;
【基金】：國(guó)家科技支撐計(jì)劃項(xiàng)目(2014BAI12B01) 國(guó)家自然科學(xué)基金(81302396) 國(guó)家臨床重點(diǎn)專(zhuān)科建設(shè)項(xiàng)目(2011-09) 廣東省科技計(jì)劃項(xiàng)目(2017A020215159) 廣東省醫(yī)學(xué)科研基金(B2016056) 廣東省職業(yè)病防治重點(diǎn)實(shí)驗(yàn)室(2012A061400007)
【分類(lèi)號(hào)】：R135.2;R-332

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