本文選題：急性肺損傷 + 血管生成素-1　； 參考：《重慶醫(yī)科大學(xué)》2013年碩士論文

【摘要】：目的：探討丹參在急性肺損傷中對血管生成素-1（angiopoietin-1,Ang-1）、血管生成素-2（angiopoietin-2,Ang-2）的調(diào)控作用及其對肺的保護(hù)機制。方法：36只清潔級SD雄性大鼠隨機分為生理鹽水對照組（NS組）、LPS組和丹參干預(yù)組。（1）對照組12只，每只大鼠從尾靜脈注入相同劑量的生理鹽水。（2）LPS組12只，，每只從尾靜脈注入LPS（5mg/kg）造成急性肺損傷模型組。（3）丹參干預(yù)組12只，每只尾靜脈注入LPS（5mg/kg）建模，1小時后再從尾靜脈注入丹參注射液10g/kg（正大青春寶藥業(yè)公司生產(chǎn)）。每組實驗大鼠在12小時后處死，收集肺泡灌洗液、血液、肺組織進(jìn)行肺濕/干重（W/D）比值、肺系數(shù)（LI）、肺通透系數(shù)（LPI）等的計算；HE染色觀察大鼠肺組織病理改變；免疫印記法測Ang-1、Ang-2在肺組織的表達(dá)。結(jié)果：LPS組及丹參組肺組織的濕/干重（W/D）比值、肺系數(shù)（LI）、肺通透系數(shù)（LPI）均較對照組明顯升高，差異具有統(tǒng)計學(xué)意義（p0.01），且病理切片都有明顯的炎癥損傷改變。Ang-1在對照組蛋白表達(dá)量較LPS組及丹參組明顯增高（p0.05），Ang-2蛋白表達(dá)量在LPS組和丹參組較對照組蛋白表達(dá)量明顯增多（p0.01）。LPS組與丹參組相比，濕/干重（W/D）比值、肺系數(shù)（LI）、肺通透系數(shù)（LPI）較丹參組升高明顯（p0.05）。結(jié)論：在急性肺損傷中，肺組織中Ang-2與Ang-1的表達(dá)調(diào)控維持肺毛細(xì)血管的穩(wěn)定起著重要作用。丹參能使急性肺損傷肺組織中Ang-2的表達(dá)減少，Ang-1的表達(dá)增多來使毛細(xì)血管的滲出減少減輕炎癥所致的肺間質(zhì)水腫、紅細(xì)胞漏出來達(dá)到肺保護(hù)作用。
[Abstract]:Aim: to investigate the effects of salvia miltiorrhiza on angiopoietin-1 Ang-1 and angiopoietin-2 Ang-2 in acute lung injury. Methods Thirty-six clean SD male rats were randomly divided into control group (n = 12) and salvia miltiorrhiza intervention group (n = 12). Acute lung injury model group was induced by injecting 5 mg / kg LPS5 mg / kg into tail vein. 12 rats were treated with salvia miltiorrhiza (LPS 5 mg / kg) and then 10 g / kg of salvia miltiorrhiza injection was injected into caudal vein for 1 hour after injection of LPS5 mg / kg into caudal vein of Salvia miltiorrhiza (Zhengyangbao Pharmaceutical Company). The rats in each group were killed 12 hours later. The lung wet / dry weight (W / D) ratio, lung coefficient (Li), lung permeability coefficient (LPI), lung permeability index (LPI), lung tissue were collected from alveolar lavage fluid, blood and lung tissue to observe the pathological changes of lung tissue. The expression of Ang-1 Ang-2 in lung tissue was measured by immunoblotting. Results compared with the control group, the wet / dry weight W / D ratio, lung coefficient Li, lung permeability index of lung tissue in the control group were significantly higher than those in the control group. The expression of Ang-1 in the control group was significantly higher than that in the LPS group and the salvia miltiorrhiza group. Compared with Salvia miltiorrhiza group, Compared with Salvia miltiorrhiza group, the wet / dry weight W / D ratio, lung coefficient, lung permeability coefficient and lung permeability coefficient were significantly higher than those of Danshen group (P < 0.05). Conclusion: the expression of Ang-2 and Ang-1 plays an important role in maintaining the stability of pulmonary capillaries in acute lung injury. Salvia miltiorrhiza can reduce the expression of Ang-2 in lung tissue of acute lung injury and increase the expression of Ang-1 so that the capillary exudation can reduce the interstitial edema caused by inflammation and the red blood cells leak out to achieve lung protection.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R563.8

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