本文選題：肺疾病 + 全自動特殊染色�。� 參考：《臨床與實驗病理學雜志》2017年06期

【摘要】：目的探討規(guī)范化特殊染色技術在肺真菌感染性疾病病理診斷中應用的意義。方法選取2011年9月~2016年3月北京朝陽醫(yī)院最終病理診斷為肺真菌感染性疾病104例,行HE染色、HE染色聯(lián)合傳統(tǒng)手工特殊染色法染過碘酸雪夫氏染色(PAS)及六胺銀和HE染色聯(lián)合全自動特殊染色法染PAS及六胺銀,將兩種特染技術規(guī)范化比較,鏡下觀察分析結果(均為初次染色結果,不包括復染結果),以最終病理診斷結果為標準,與臨床初診結果比較。結果肺真菌感染性疾病確診率和真菌分型率,從低到高順序一致,為臨床初步診斷(29.8%和19.2%)、HE染色法(32.7%和32.7%)、HE染色聯(lián)合傳統(tǒng)手工特殊染色法染PAS和六胺銀(90.4%和87.5%)、HE染色聯(lián)合全自動特殊染色法染PAS和六胺銀(98.1%和94.2%)。這4種方法從兩方面比較差異有統(tǒng)計學意義(P0.01,P0.01),第4種方法與前兩種比較方法差異有統(tǒng)計學意義(P0.01,P0.01),與第3種方法比較確診率差異有統(tǒng)計學意義(P0.05),分型率差異無統(tǒng)計學意義(P0.05)。但全自動特殊染色法染PAS及六胺銀操作步驟更簡單,化學試劑規(guī)范化,無人工和環(huán)境因素影響,耗時短,脫片和復染張數(shù)更少。結論 HE染色法聯(lián)合全自動特殊染色法染PAS和六胺銀更加規(guī)范化,有助于提高肺真菌感染性疾病的確診率和真菌分型率。
[Abstract]:Objective to explore the significance of standardized special staining technique in pathological diagnosis of pulmonary fungal infections. Methods 104 cases of pulmonary fungal infection were selected from September 2011 to March 2016 in Beijing Chaoyang Hospital. He and HE staining combined with traditional manual special staining were used to stain Pass and HE staining combined with automatic special staining for PAS and silver hexamine. The two special staining techniques were compared with each other. The results of microscopic observation and analysis (all of them were primary staining results, excluding the results of repeated staining) were compared with the results of clinical initial diagnosis according to the criteria of final pathological diagnosis. Results the rates of diagnosis and classification of pulmonary fungal infectious diseases were consistent from low to high. To make a preliminary diagnosis of 29.8% and 19.2% of them by HE staining, 32.7% and 32.7% of them were stained with PAS, 90.4% and 87.5% of them by traditional manual special staining, and 98.1% and 94.2% of them were stained with PAS and 94.1% and 94.2% respectively by fully automatic special staining. There were significant differences between the four methods in two aspects, the fourth method had statistical significance compared with the former two methods, and the difference between the four methods and the third method had statistical significance (P0.05A), but there was no significant difference in the classification rate between the four methods and the former two methods (P0.055.There was no significant difference in the classification rate between the four methods and the third method). However, the procedure of automatic special staining for PAS and hexamethylamine silver is simpler, the chemical reagent is standardized, the time is short, the number of strip and multiple dye is less, the time is short without the influence of artificial and environmental factors. Conclusion HE staining combined with automatic special staining for PAS and silver hexamine is more standardized, which is helpful to improve the rate of diagnosis and classification of pulmonary fungal infections.
【作者單位】： 首都醫(yī)科大學附屬朝陽醫(yī)院病理科;
【分類號】：R519

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