發(fā)布時(shí)間：2018-05-03 21:23

  本文選題：慢性阻塞性肺疾病 + 聚合酶鏈?zhǔn)椒磻?yīng)�。� 參考：《山西醫(yī)科大學(xué)》2013年碩士論文

【摘要】：目的探討解整合素-金屬蛋白酶33(ADAM33)基因單核苷酸多態(tài)性(SNPs)與山西漢族人群慢性阻塞性肺疾病(COPD)發(fā)病的相關(guān)性。 方法對(duì)100例健康者(對(duì)照組)、103例COPD患者(病例組),利用聚合酶鏈?zhǔn)椒磻?yīng)(PCR)以及DNA測(cè)序的方法,對(duì)ADAM33基因的BC+1和F+1位點(diǎn)多態(tài)性與COPD及肺功能的關(guān)系進(jìn)行統(tǒng)計(jì)學(xué)分析。 結(jié)果ADAM33基因BC+1位點(diǎn)3種基因型AA.AG.GG在對(duì)照組中分布為17.0%、56.0%、27.0%,在病例組中分布為15.5%、45.6%、38.8%,差異無(wú)統(tǒng)計(jì)學(xué)意義(x2=3.296,P0.05),其等位基因A和G的頻率在對(duì)照組分別為45.5%、55.0%,病例組中分別為38.3%、61.7%,差異無(wú)統(tǒng)計(jì)學(xué)意義(x2=1.847,P0.05)。 ADAM33基因F+1位點(diǎn)3種基因型AA、AG、GG在對(duì)照組中分布為7.0%、51.0%、42.0%,在病例組中分布為20.4%、38.8%和40.8%,差異有統(tǒng)計(jì)學(xué)意義(x2=8.287,P0.05),其等位基因A和G的頻率在對(duì)照組分別為32.5%、67.5%,在病例組中分別為39.8%、60.2%,差異無(wú)統(tǒng)計(jì)學(xué)意義(x2=2.345,P0.05)。 ADAM33基因的F+1位點(diǎn)多態(tài)性與肺功能的關(guān)系：該位點(diǎn)3種基因型間FEV1/FVC(%)比較,差異有統(tǒng)計(jì)學(xué)意義(F=2.837,P0.05)；但是與FEV1預(yù)計(jì)值(%)比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(F=0.396,P0.05)。 結(jié)論BC+1位點(diǎn)與該人群COPD的發(fā)病關(guān)系不明顯；但是F+1位點(diǎn)對(duì)山西漢族人群COPD發(fā)病有一定的影響；F+1位點(diǎn)的多態(tài)性可能與肺功能有關(guān)指標(biāo)的下降有關(guān)聯(lián)。
[Abstract]:Objective To investigate the association between single nucleotide polymorphism ( SNPs ) of integrin - metal proteinase 33 ( SNPs ) and chronic obstructive pulmonary disease ( COPD ) in Shanxi Han population .

Methods 100 healthy subjects ( control group ) , 103 patients with COPD ( case group ) , polymerase chain reaction ( PCR ) and DNA sequencing were used to analyze the relationship between the polymorphism of BC + 1 and F + 1 sites in patients with COPD and lung function .

Results The three genotypes AA , AG and GG in the BC + 1 locus were 17.0 % , 56.0 % and 27.0 % in the control group , 15.5 % , 45.6 % and 38.8 % in the case group respectively . The frequencies of the alleles A and G were 45.3 % and 61.7 % in the control group , respectively , and the difference was not statistically significant ( x2 = 1.847 , P0.05 ) .

The frequencies of the alleles A and G of the three genotypes AA , AG and GG were 7.0 % , 51.0 % and 42.0 % in the control group . The frequencies of the alleles A and G were 32.5 % , 67.2 % in the control group and 39.8 % and 60.2 % respectively in the case group ( x2 = 2.345 , P0.05 ) .

The relationship between the polymorphism of the F + 1 locus and the lung function was found between the three genotypes : FEV1 / FVC ( % ) in the three genotypes ( F = 2.837 , P0.05 ) .
However , the difference was not statistically significant compared to predicted FEV1 ( % ) ( F = 0.396 , P0.05 ) .

Conclusion The BC + 1 locus is not related to the pathogenesis of COPD .
However , the F + 1 locus had some effects on COPD in the Han population in Shanxi Province .
The F + 1 site polymorphism may be associated with a decrease in lung function - related indicators .

【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R563.9

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 羅鳳鳴,李雙慶,王曾禮;解整合素-金屬蛋白酶與哮喘[J];國(guó)外醫(yī)學(xué).呼吸系統(tǒng)分冊(cè);2005年04期

2 方怡,冉丕鑫;腺病毒感染與慢性阻塞性肺疾病發(fā)病機(jī)制研究現(xiàn)狀[J];國(guó)外醫(yī)學(xué).呼吸系統(tǒng)分冊(cè);2005年06期

3 張?jiān)f,何農(nóng)躍,陸祖宏;基因芯片的PCR引物設(shè)計(jì)[J];化工時(shí)刊;2002年07期

4 余國(guó)輝;陳敏;;慢性阻塞性肺疾病發(fā)病機(jī)制的發(fā)展?fàn)顩r[J];臨床肺科雜志;2010年01期

5 劉清敏,劉景艷;炎癥細(xì)胞與COPD發(fā)病機(jī)制的關(guān)系[J];中原醫(yī)刊;2004年19期

6 揭志軍;金美玲;蔡映云;袁正宏;胡蕓文;徐楊;;白介素-4和白介素-13促進(jìn)人肺成纖維細(xì)胞ADAM33基因的表達(dá)[J];復(fù)旦學(xué)報(bào)(醫(yī)學(xué)版);2006年03期

7 張忠魯;徐立新;;COPD的研究現(xiàn)狀[J];中國(guó)實(shí)用內(nèi)科雜志;2006年18期

8 張偉兵;王欣燕;田曉彥;張慧;王志鵬;沈巖;高玉艷;;ADAM33基因多態(tài)性影響慢性阻塞性肺疾病氣道炎癥[J];中國(guó)呼吸與危重監(jiān)護(hù)雜志;2012年01期

9 蔡柏薔;慢性阻塞性肺疾病發(fā)病機(jī)制的新學(xué)說(shuō):肺炎衣原體慢性感染[J];中華結(jié)核和呼吸雜志;2000年02期

10 胡國(guó)平;彭公永;胡錦興;冉丕鑫;;腫瘤壞死因子α-308基因多態(tài)性與慢性阻塞性肺疾病易感性的薈萃分析[J];中華結(jié)核和呼吸雜志;2007年08期

，

本文編號(hào)：1840155