本文選題：呼吸道合胞病毒 + 哮喘��； 參考：《廣東醫(yī)學(xué)》2017年21期

【摘要】：目的使用人類全轉(zhuǎn)錄組芯片檢測(cè)呼吸道合胞病毒(RSV)誘發(fā)哮喘中的樹(shù)突狀細(xì)胞(DCs),以篩選差異表達(dá)的長(zhǎng)鏈非編碼RNAs(lncRNAs)。在lncRNAs轉(zhuǎn)錄調(diào)控層面研究RSV誘發(fā)哮喘的機(jī)制。方法設(shè)立HDC-BEAS+RSV組、HDC-RSV組和HDC-CONTROL組3組,其中HDC-BEAS+RSV組是將RSV感染的人支氣管上皮細(xì)胞BEAS-2B和未成熟DCs通過(guò)Transwell共培養(yǎng),HDC-RSV組是將RSV感染未成熟DCs,HDCCONTROL組是將RSV對(duì)照液感染未成熟DCs。使用affymatrix的HTA2.0芯片檢測(cè)3組DCs中基因和lncRNAs的表達(dá)差異,并對(duì)其進(jìn)行聚類分析、GO分析和Pathway分析。結(jié)果篩選出有差異表達(dá)的25個(gè)基因和29個(gè)lncRNAs,其中20個(gè)基因表達(dá)上調(diào),5個(gè)基因表達(dá)下調(diào),27個(gè)lncRNAs表達(dá)上調(diào),2個(gè)lncRNAs表達(dá)下調(diào)。上調(diào)的基因主要涉及促炎免疫反應(yīng),例如信號(hào)轉(zhuǎn)導(dǎo)、趨化因子信號(hào)傳導(dǎo)途徑、細(xì)胞因子受體相互作用等。結(jié)論我們篩選出了RSV誘發(fā)哮喘中差異表達(dá)的lncRNAs,為進(jìn)一步在lncRNA轉(zhuǎn)錄調(diào)控層面研究RSV誘發(fā)哮喘的機(jī)制奠定基礎(chǔ)。
[Abstract]:Objective to detect dendritic cells (DC) in asthma induced by respiratory syncytial virus (RSVV) using human whole transcription microarray to screen differentially expressed long chain noncoding RNAsC RNAsS. To study the mechanism of asthma induced by RSV at the level of lncRNAs transcriptional regulation. Methods HDC-BEAS RSV group (HDC-RSV) and HDC-CONTROL group (3 groups) were established. The HDC-BEAS RSV group infects RSV infected human bronchial epithelial cells BEAS-2B and immature DCs through Transwell coculture. The HDC-BEAS RSV group infects immature DC-RSV with RSV and infects immature DC-RSV with RSV control fluid. The difference of gene and lncRNAs expression in three groups of DCs was detected by HTA2.0 chip of affymatrix, and then cluster analysis and Pathway analysis were carried out. Results Twenty-five differentially expressed genes and 29 lncRNAss were screened. Among them, 20 genes were up-regulated, 5 genes were down-regulated, 27 lncRNAs expressions were up-regulated and 2 lncRNAs expressions were down-regulated. The up-regulated genes are mainly involved in pro-inflammatory immune response, such as signal transduction, chemokine signaling pathway, cytokine receptor interaction and so on. Conclusion We screened out the differential expression of lncRNAsin in asthma induced by RSV and laid a foundation for further study on the mechanism of RSV induced asthma at the level of transcriptional regulation of lncRNA.
【作者單位】： 上海交通大學(xué)醫(yī)學(xué)院附屬第九人民醫(yī)院呼吸內(nèi)科;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(編號(hào):81170028)
【分類號(hào)】：R562.25

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 謝民;;由呼吸道合胞病毒和金葡菌混合感染所致之成人致死性出血性肺炎[J];國(guó)外醫(yī)學(xué)(微生物學(xué)分冊(cè));1983年02期

2 謝健屏，常汝虛，張美德;從尸解肺組織分離呼吸道合胞病毒2例分析[J];中華實(shí)驗(yàn)和臨床病毒學(xué)雜志;1994年01期

3 申志強(qiáng),馮英璞,孫俊秀;呼吸道合胞病毒肺炎98例臨床觀察[J];中原醫(yī)刊;1994年05期

4 呂啟圣，周光延，龔明敏，閆田玉，，宗平平，張忠浩，楊春霞，鄭企靜，劉玉華，富長(zhǎng)玲，李佳，趙高潮;呼吸道合胞病毒肺炎623例臨床分析[J];北京醫(yī)學(xué);1995年01期

5 孫本韜;;呼吸道合胞病毒肺炎超微病理特征[J];中國(guó)中西醫(yī)結(jié)合雜志;1993年10期

6 袁孝賓;成人可群體獲得性發(fā)生呼吸道合胞病毒肺炎[J];國(guó)外醫(yī)學(xué).呼吸系統(tǒng)分冊(cè);1997年03期

7 曹玲;呼吸道合胞病毒肺炎[J];中國(guó)醫(yī)刊;2004年03期

8 陳瑞芬,竺慧琴,王一心,鄒如t

本文編號(hào)：1795981