發(fā)布時間：2018-04-24 08:55

  本文選題：呼吸道合胞病毒 + 哮喘�。� 參考：《廣東醫(yī)學》2017年21期

【摘要】：目的使用人類全轉(zhuǎn)錄組芯片檢測呼吸道合胞病毒(RSV)誘發(fā)哮喘中的樹突狀細胞(DCs),以篩選差異表達的長鏈非編碼RNAs(lncRNAs)。在lncRNAs轉(zhuǎn)錄調(diào)控層面研究RSV誘發(fā)哮喘的機制。方法設立HDC-BEAS+RSV組、HDC-RSV組和HDC-CONTROL組3組,其中HDC-BEAS+RSV組是將RSV感染的人支氣管上皮細胞BEAS-2B和未成熟DCs通過Transwell共培養(yǎng),HDC-RSV組是將RSV感染未成熟DCs,HDCCONTROL組是將RSV對照液感染未成熟DCs。使用affymatrix的HTA2.0芯片檢測3組DCs中基因和lncRNAs的表達差異,并對其進行聚類分析、GO分析和Pathway分析。結(jié)果篩選出有差異表達的25個基因和29個lncRNAs,其中20個基因表達上調(diào),5個基因表達下調(diào),27個lncRNAs表達上調(diào),2個lncRNAs表達下調(diào)。上調(diào)的基因主要涉及促炎免疫反應,例如信號轉(zhuǎn)導、趨化因子信號傳導途徑、細胞因子受體相互作用等。結(jié)論我們篩選出了RSV誘發(fā)哮喘中差異表達的lncRNAs,為進一步在lncRNA轉(zhuǎn)錄調(diào)控層面研究RSV誘發(fā)哮喘的機制奠定基礎。
[Abstract]:Objective to detect dendritic cells (DC) in asthma induced by respiratory syncytial virus (RSVV) using human whole transcription microarray to screen differentially expressed long chain noncoding RNAsC RNAsS. To study the mechanism of asthma induced by RSV at the level of lncRNAs transcriptional regulation. Methods HDC-BEAS RSV group (HDC-RSV) and HDC-CONTROL group (3 groups) were established. The HDC-BEAS RSV group infects RSV infected human bronchial epithelial cells BEAS-2B and immature DCs through Transwell coculture. The HDC-BEAS RSV group infects immature DC-RSV with RSV and infects immature DC-RSV with RSV control fluid. The difference of gene and lncRNAs expression in three groups of DCs was detected by HTA2.0 chip of affymatrix, and then cluster analysis and Pathway analysis were carried out. Results Twenty-five differentially expressed genes and 29 lncRNAss were screened. Among them, 20 genes were up-regulated, 5 genes were down-regulated, 27 lncRNAs expressions were up-regulated and 2 lncRNAs expressions were down-regulated. The up-regulated genes are mainly involved in pro-inflammatory immune response, such as signal transduction, chemokine signaling pathway, cytokine receptor interaction and so on. Conclusion We screened out the differential expression of lncRNAsin in asthma induced by RSV and laid a foundation for further study on the mechanism of RSV induced asthma at the level of transcriptional regulation of lncRNA.
【作者單位】： 上海交通大學醫(yī)學院附屬第九人民醫(yī)院呼吸內(nèi)科;
【基金】：國家自然科學基金資助項目(編號:81170028)
【分類號】：R562.25

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