發(fā)布時(shí)間：2018-04-21 13:43

  本文選題：IL-4 + IL-13��； 參考：《華中科技大學(xué)》2013年碩士論文

【摘要】：研究背景： CXCR4存在于機(jī)體多種細(xì)胞表面，是趨化因子SDF-1α的特異性受體，在細(xì)胞的增殖和遷移中起著重要作用。有研究證實(shí)CXCR4及其配體SDF-1α參與了哮喘的氣道炎癥和氣道高反應(yīng)，在哮喘中發(fā)揮重要作用。Th2細(xì)胞因子IL-4和IL-13在哮喘的發(fā)病機(jī)制過程中扮演了重要角色，嗜酸性粒細(xì)胞表面CXCR4的表達(dá)在IL-4和IL-13的影響下有所改變。而哮喘中支氣管上皮細(xì)胞是否表達(dá)CXCR4以及其表達(dá)是否受Th2細(xì)胞因子IL-4和IL-13的影響，，目前尚未有研究報(bào)道。 目的： 探討支氣管上皮細(xì)胞在Th2細(xì)胞因子環(huán)境下是否表達(dá)CXCR4以及其表達(dá)是否受IL-4和IL-13的影響。 方法： 本實(shí)驗(yàn)通過用六孔板培養(yǎng)人支氣管上皮細(xì)胞系，饑餓16h，然后用IL-4（20ng/ml）和IL-13（20ng/ml）分別及聯(lián)合刺激支氣管上皮細(xì)胞24h，形成Th2細(xì)胞因子環(huán)境，以體外模擬哮喘環(huán)境。通過提取蛋白，電泳，把丙烯酰胺凝膠中的蛋白轉(zhuǎn)移到PVDF膜上，封閉1h，CXCR4單克隆抗體抗體孵育過夜，洗滌后加入二抗孵育1h，洗滌，ECL顯色、曝光。 結(jié)果： 正常組HBEsCXCR4蛋白表達(dá)灰度比值為（0.54±0.07），而IL-4刺激組CXCR4蛋白表達(dá)灰度比值為（0.91±0.07），明顯高于正常組（P0.05），具有統(tǒng)計(jì)學(xué)差異。IL-13刺激組CXCR4蛋白表達(dá)灰度比值為（1.11±0.11），與正常組比較，也明顯增高（P0.05），差異具有統(tǒng)計(jì)學(xué)意義。IL-4+IL-13聯(lián)合刺激組CXCR4蛋白表達(dá)灰度比值（1.19±0.18），明顯高于正常組（P0.05），具有統(tǒng)計(jì)學(xué)差異。 結(jié)論： 該實(shí)驗(yàn)結(jié)果表明哮喘環(huán)境中支氣管上皮細(xì)胞表面CXCR4蛋白表達(dá)上調(diào)，Th2細(xì)胞因子IL-4和IL-13均可使支氣管上皮細(xì)胞表面CXCR4表達(dá)增高，說明其表達(dá)受到Th2細(xì)胞因子的調(diào)控。
[Abstract]:Background: CXCR4 is a specific receptor of chemokine SDF-1 偽 and plays an important role in cell proliferation and migration. It has been confirmed that CXCR4 and its ligand SDF-1 偽 are involved in airway inflammation and airway hyperresponsiveness in asthma. Th2 cytokines, IL-4 and IL-13, play an important role in the pathogenesis of asthma. The expression of CXCR4 on eosinophil surface was affected by IL-4 and IL-13. However, whether bronchial epithelial cells express CXCR4 and whether their expression is affected by Th2 cytokines, IL-4 and IL-13, has not been reported. Objective: To investigate whether bronchial epithelial cells express CXCR4 in the environment of Th2 cytokines and whether their expression is affected by IL-4 and IL-13. Methods: In this experiment, human bronchial epithelial cell line was cultured with six-hole plate for 16 h, then stimulated with IL-4 + 20 ng / ml and IL-13 + 20 ng / ml for 24 h, respectively, to form Th2 cytokine environment, and to simulate asthma in vitro. The protein in acrylamide gel was transferred to PVDF membrane by extracting protein and electrophoretic. The monoclonal antibody against CXCR4 was incubated overnight after washing, then the second antibody was added for 1 hour. Results: The gray level ratio of HBEsCXCR4 protein expression in normal group was 0.54 鹵0.07, while that of CXCR4 protein in IL-4 stimulation group was 0.91 鹵0.07, which was significantly higher than that in normal group (P 0.05). The ratio of CXCR4 protein expression in IL-13 stimulated group was 1.11 鹵0.11, which was significantly higher than that in normal group. The grayscale ratio of CXCR4 protein expression in IL-4 IL-13 combined stimulation group was 1.19 鹵0.18, which was significantly higher than that in normal control group (P 0.05). Conclusion: The results showed that the expression of Th _ 2 cytokine IL-4 and IL-13 could increase the expression of CXCR4 on the surface of bronchial epithelial cells in asthmatic environment, which indicated that the expression of Th2 cytokines regulated the expression of Th _ 2 cytokines.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R562.25

【共引文獻(xiàn)】

相關(guān)期刊論文 前9條

1 劉蕊;;嬰幼兒支氣管哮喘發(fā)病機(jī)制中白介素-4及維生素D_3的研究進(jìn)展[J];內(nèi)蒙古醫(yī)學(xué)雜志;2011年S5期

2 金璇;趙學(xué)龍;;清肺平喘膠囊治療哮喘的作用機(jī)制研究[J];南京中醫(yī)藥大學(xué)學(xué)報(bào);2013年05期

3 吳文娟;董曉斐;李嬌艷;陳春輝;周婭薇;;咳喘寧?kù)F化吸入對(duì)哮喘大鼠肺泡灌洗液白介素-5與嗜酸性粒細(xì)胞的影響[J];湖南中醫(yī)藥大學(xué)學(xué)報(bào);2014年01期

4 朱曉潔;王s

本文編號(hào)：1782685