發(fā)布時間：2018-04-14 02:31

  本文選題：微小RNA- + 慢性阻塞性肺疾病�。� 參考：《中南大學(xué)學(xué)報(醫(yī)學(xué)版)》2016年03期

【摘要】：目的:通過研究mi R-21在慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)患者外周血清及單個核細胞中的表達,探討mi R-21在COPD發(fā)病中的意義及機制。方法:將研究對象分為健康對照組(n=41)和COPD組(n=49),收集兩組外周血清,分離其外周血單個核細胞并收集臨床資料。運用實時熒光定量PCR法檢測各組外周血清及單個核細胞中mi R-21的表達水平。分析COPD組mi R-21與肺功能的關(guān)系,并對mi R-21與COPD患者一秒用力呼氣容積(forced expiratory volume in one second,FEV1)進行相關(guān)性分析。結(jié)果:COPD組外周血清及單個核細胞中mi R-21表達水平較健康對照組增高;COPD外周血清及單個核細胞中mi R-21差異表達與肺功能相關(guān);COPD患者mi R-21表達水平與FEV1呈正相關(guān)。結(jié)論:COPD患者外周血清及單個核細胞中mi R-21表達升高可能與COPD發(fā)病過程及病情嚴(yán)重程度相關(guān)。
[Abstract]:Objective: through the research of MI R-21 in patients with chronic obstructive pulmonary disease (chronic obstructive pulmonary disease, COPD) expression in serum and mononuclear cells in the patients, and to explore the significance and mechanism of MI R-21 in the pathogenesis of COPD. Methods: the subjects were divided into healthy control group (n= 41) and COPD group (n=49) two, collect serum group of peripheral isolated peripheral blood mononuclear cells and clinical data were collected. The expression level of serum and MI in mononuclear cells of R-21 were detected by real-time fluorescence quantitative PCR method. The relationship between peripheral R-21 analysis group COPD MI and pulmonary function, and the MI of R-21 and COPD in patients with a second forced expiratory volume (forced expiratory volume in one second, FEV1) were analyzed. Results: in COPD group, serum and peripheral blood mononuclear cells Mi expression level of R-21 increased compared with the healthy control group; and the expression of COPD in peripheral blood mononuclear cells in MI and R-21 between Lung function is correlated. The expression level of MI R-21 is positively correlated with FEV1 in COPD patients. Conclusion: the increase of MI R-21 expression in peripheral serum and mononuclear cells of COPD patients may be related to the pathogenesis and severity of COPD.

【作者單位】： 中南大學(xué)湘雅三醫(yī)院呼吸科;長沙市第四醫(yī)院呼吸科;
【基金】：國家自然科學(xué)基金青年基金(81500033)~~
【分類號】：R563.9

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