本文選題：急性呼吸窘迫綜合征　切入點(diǎn)：血小板　出處：《中國呼吸與危重監(jiān)護(hù)雜志》2017年01期

【摘要】：目的探討急性呼吸窘迫綜合征(ARDS)發(fā)病的分子機(jī)制以及血小板活化的信號通路。方法將30只健康SD大鼠隨機(jī)分為5組。其中實(shí)驗(yàn)組4組,尾靜脈注射油酸(0.25 ml/kg)制備ARDS模型;對照組1組,尾靜脈注射等量生理鹽水。在實(shí)驗(yàn)組注射油酸后2、6、24、72 h,對照組注射生理鹽水后2 h,處死大鼠,從腹主動脈采血,分離血小板,提取血小板蛋白,用免疫印跡法檢測動物血小板內(nèi)絲裂原活化蛋白激酶激酶3(MKK3)的磷酸化水平變化,了解ARDS時(shí)血小板絲裂原活化蛋白激酶(MAPKs)信號通路的改變以及與ARDS發(fā)病的關(guān)系。結(jié)果注射油酸后6~72 h大鼠血小板的MKK3磷酸化水平明顯增高(P0.05),其中6 h組為對照組的2.4倍(0.50±0.09 vs.0.21±0.05);24 h組表達(dá)量最高(0.78±0.06),為對照組的3.7倍;72 h組稍有回落(0.75±0.13),但仍明顯高于對照組。結(jié)論 ARDS時(shí)血小板的活化過程與MKK3-p38 MAPK信號轉(zhuǎn)導(dǎo)通路的啟動有關(guān)。
[Abstract]:Objective to investigate the molecular mechanism of acute respiratory distress syndrome (ARDS) and the signal pathway of platelet activation. Methods Thirty healthy SD rats were randomly divided into five groups: experimental group (n = 4) and caudal vein injection of oleic acid 0.25 ml / kg to establish ARDS model. The rats in the control group were killed 2 hours after the injection of oleic acid, the rats were killed at 2 h after injection of oleic acid, the blood was collected from the abdominal aorta, platelets were isolated and platelet protein was extracted. The phosphorylation of mitogen-activated protein kinase 3 (MKK3) in animal platelets was detected by Western blot. To investigate the changes of mitogen-activated protein kinase (MAPKs) signaling pathway in platelets during ARDS and its relationship with the pathogenesis of ARDS. Results the level of MKK3 phosphorylation in platelets of rats at 6h after oleic acid injection was significantly higher than that of control group (P 0.05). The highest expression level was 0.78 鹵0.06 in the 24 h group with 0.50 鹵0.09 vs.0.21 鹵0.05 vs.0.21 鹵0.05, which was a little lower than that in the control group at 72 h, but still significantly higher than that in the control group. Conclusion the activation process of platelet during ARDS is related to the initiation of MKK3-p38 MAPK signal transduction pathway.
【作者單位】： 山西大同大學(xué)呼吸病與職業(yè)病研究所;臨汾市中心醫(yī)院呼吸科;
【基金】：山西省自然科學(xué)基金(2013011055-5) 大同市基礎(chǔ)研究項(xiàng)目(2014105-2)
【分類號】：R563.8

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