本文選題：人體呼出氣　切入點：相對高分子量　出處：《高等學�；瘜W學報》2017年05期 　論文類型：期刊論文

【摘要】：二次電噴霧電離源耦合超高分辨質譜(SESI-UHRMS)有望檢出人體呼出氣中分子量大于300的相對高分子量化合物,這些化合物的發(fā)現將有助于更準確地理解呼出氣中揮發(fā)性有機化合物(VOCs)的來源、產生機制以及SESI源電離機理,更好地實現SESI-UHRMS的轉化應用.本研究自組裝nanoSESI源(尚無商業(yè)產品)耦合四極桿-靜電場軌道阱質譜(最高質量分辨率1.2×10~5),考察了該裝置對健康人體呼出氣中分子量為300~500化合物的檢出情況.結果表明,所搭建的nanoSESI-UHRMS裝置檢測人體呼出氣的重現性好、靈敏度高,可檢出數十種分子量為300~500的化合物.根據這些化合物在單次呼氣過程中信號強度隨時間變化的趨勢,推斷其來源分別為內源性和外源性;各化合物的元素組成主要包括C,H,N和O,環(huán)-雙鍵當量(RDB)的均值為(4.5±3.1),表明檢出的化合物可能為醛酮或不飽和脂肪酸,容易在SESI源中被電離.本研究初步驗證了自組建nanoSESI-UHRMS檢測人體呼出氣中相對高分子量化合物的可行性,為后續(xù)進一步開展方法應用奠定了基礎.
[Abstract]:The secondary electrospray ionization source coupled with ultra-high resolution mass spectrometry (SESI-UHRMS) is expected to detect relative high molecular weight compounds with molecular weight greater than 300 in human exhalation. The discovery of these compounds will contribute to a more accurate understanding of the sources, production mechanisms and ionization mechanisms of volatile organic compounds (VOCs) in exhaled air, The conversion application of SESI-UHRMS is better realized. In this study, the self-assembled nanoSESI source (without commercial products) coupled with quadrupole electrostatic field orbital well mass spectrometry (maximum mass resolution 1.2 脳 10 ~ (5)) has been studied. The molecular weight of the device in the exhalation and exhalation of healthy human is investigated. The results show that, The nanoSESI-UHRMS device has good reproducibility and high sensitivity in detecting exhalation, and can detect dozens of compounds with molecular weight of 300 ~ 500. According to the trend of signal intensity changing with time during a single exhalation, It was inferred that the compounds originated from endogenous and exogenous sources respectively, and their elemental compositions mainly included Con HN and O, and the mean value of RDBs was 4.5 鹵3.1, indicating that the compounds detected might be aldehydes, ketones or unsaturated fatty acids. It is easy to be ionized in SESI source. This study preliminarily verifies the feasibility of self-made nanoSESI-UHRMS for the detection of relative high molecular weight compounds in human exhaled air, which lays a foundation for the further application of this method.
【作者單位】： 暨南大學質譜儀器與大氣環(huán)境研究所廣東省大氣污染在線源解析系統工程技術研究中心;暨南大學第一附屬醫(yī)院;
【基金】：中央高�；究蒲袠I(yè)務費專項資金(批準號:21615457)資助~~
【分類號】：O657.63;R56

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本文編號：1639218