本文選題：支氣管哮喘　切入點：單核苷酸多態(tài)性（SNP）　出處：《大連醫(yī)科大學》2013年碩士論文　論文類型：學位論文

【摘要】：目的： 白細胞介素17（interleukin17，IL-17）由T細胞亞群——輔助性T細胞17(Thelp cell17, Th17)分泌產(chǎn)生。IL-17家族中的成員之一IL-17A在變應(yīng)性炎癥中具有重要的作用[1]，與支氣管哮喘的發(fā)生、發(fā)展有關(guān)。IL-17G-152A基因多態(tài)性與自身免疫性疾病的相關(guān)性研究較多，然而對IL-17G-152A基因多態(tài)性與哮喘關(guān)系的研究甚少。本實驗旨在通過檢測哮喘患者外周血IL-17G-152A的基因表達，從而探討IL-17G-152A單核苷酸多態(tài)性（single nucleotide polymorphism,SNP)在哮喘發(fā)病機制中的作用及中國東北地區(qū)漢族人群IL-17G-152A多態(tài)性(rs2275913)與哮喘發(fā)病的相關(guān)性。 方法： 采用病例對照研究方法，共收集103例哮喘患者和48例健康對照組。采集外周靜脈血，提取全血基因組DNA，用Real-time PCR方法檢測IL-17G-152A(rs2275913)SNP。計量資料以均數(shù)±標準差（x±s）表示，符合正態(tài)分布數(shù)據(jù)采用樣本均數(shù)t檢驗。計數(shù)資料以構(gòu)成比表示，，組間基因型頻率等計數(shù)資料的比較采用χ~2檢驗，單變量Logistic回歸分析探討等位基因、基因型與哮喘發(fā)病的相關(guān)性。 結(jié)果： 1. IL-17G-152A位點在中國東北地區(qū)存在多態(tài)性，各組基因型頻率符合Hardy-Weinberg平衡。健康人群A、G等位基因頻率分別45.8％、54.2％；AA、AG和GG基因型頻率分別20.8％、50％、29.2％。 2．哮喘組IL-17G-152A位點等位基因A（χ~2=0.108，P=0.742＞0.05）及AA基因型頻率（χ~2=0.977，P=0.614＞0.05）與正常對照組比較無統(tǒng)計學差異。 3．外周血嗜酸性粒細胞、血清總IgE、基礎(chǔ)肺功能FEV1/pred%、FEV1/FVC%異常個體在各基因型組間比較差異無統(tǒng)計學意義（P＞0.05）。 4.單變量Logistic回歸分析探討等位基因、基因型與哮喘發(fā)病的相關(guān)性，P值均＞0.05，對疾病均沒有顯著性影響�；貧w模型對疾病變異的貢獻是6%。 結(jié)論： 1. IL-17G-152A位點在中國東北地區(qū)漢族人群中存在多態(tài)性。 2. IL-17G-152A單核苷酸多態(tài)性與中國東北地區(qū)哮喘發(fā)病無關(guān)，未發(fā)現(xiàn)A等位基因及AA基因攜帶者罹患哮喘的風險增加，即rs2275913SNP并非哮喘的易感位點。 3.IL-17G-152A基因多態(tài)性與外周血嗜酸性粒細胞、血清總IgE、肺功能FEV1/pred%、FEV1/FVC%異常個體的比例無明顯相關(guān)性。
[Abstract]:Objective:. Interleukin 17 interleukin 17 (IL-17) is secreted by T cell subsets, helper T cell 17T help cell 17 (Th17) to produce IL-17A, one of the members of the IL-17 family, which plays an important role in allergic inflammation [1], and is associated with the occurrence of bronchial asthma. The relationship between the polymorphism of IL-17G-152A gene and autoimmune diseases was studied, but the relationship between the polymorphism of IL-17G-152A gene and asthma was not studied. The aim of this study was to detect the gene expression of IL-17G-152A in peripheral blood of asthmatic patients. To explore the role of single nucleotide polymorphisms (IL-17G-152A) in the pathogenesis of asthma and the relationship between IL-17G-152A polymorphism rs2275913) and asthma. Methods:. Using a case-control study, 103 asthmatic patients and 48 healthy controls were collected. Peripheral venous blood was collected and genomic DNA was extracted from the whole blood. IL-17G-152Atran rs2275913 SNPs were detected by Real-time PCR. The measured data were expressed as mean 鹵standard deviation (x 鹵s). According to the normal distribution data, the sample mean t test was used. The counting data were expressed as composition ratio, the genotypes frequency and other counting data were compared by 蠂 ~ 2 test, and the alleles were analyzed by univariate Logistic regression analysis. Relationship between genotype and asthma. Results:. 1. The polymorphism of IL-17G-152A locus was found in Northeast China, and the allele frequencies of AHG in each group were consistent with Hardy-Weinberg balance. The allele frequencies of AHG in healthy population were 45.8and 54.2GG, respectively. The frequencies of AA AG and GG genotype were 20.850 and 29.229.2 respectively. 2. There was no significant difference in the allele A of IL-17G-152A locus between asthma group and normal control group (蠂 2 + 0.108) and the frequency of AA genotype (蠂 2 + 0.97 7 P0. 614 > 0. 05). 3.Peripheral blood eosinophilic granulocytes, serum total IgE, FEV 1 / pred% abnormal individuals had no significant difference among the genotypes (P > 0.05). 4. Univariate Logistic regression analysis was used to study alleles. The correlation between genotype and asthma was more than 0.05, which had no significant effect on disease. The contribution of regression model to disease variation was 6%. Conclusion:. 1. The polymorphism of IL-17G-152A locus was found in the Han population in Northeast China. 2. The single nucleotide polymorphisms of IL-17G-152A were not associated with asthma in Northeast China. There was no increased risk of asthma in A allele and AA gene carriers, that is, rs2275913SNP was not a susceptible site for asthma. 3. The polymorphism of IL-17G-152A gene had no significant correlation with the proportion of individuals with abnormal FEV 1 / FVC% in peripheral blood eosinophil, serum total IgE and pulmonary function FEV 1 / pred.
【學位授予單位】：大連醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R562.25

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相關(guān)期刊論文 前2條

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