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  本文選題：小鼠　切入點：支氣管哮喘模型　出處：《華中科技大學學報(醫(yī)學版)》2014年02期 　論文類型：期刊論文

【摘要】：目的探討滴鼻和霧化兩種不同激發(fā)方式對小鼠支氣管哮喘模型氣道炎癥的影響,為研究和優(yōu)化支氣管哮喘動物模型提供實驗和理論依據(jù)。方法選擇雞卵清蛋白(OVA)致敏的BALB/c小鼠,分別采用滴鼻和霧化兩種不同方式進行激發(fā),復(fù)制哮喘模型,觀察支氣管肺泡灌洗液(BALF)中白細胞總數(shù)和細胞分類計數(shù),ELISA方法檢測BALF上清中IL-4、IL-13含量,常規(guī)病理切片蘇木精-伊紅(HE)染色觀察肺內(nèi)炎癥情況。結(jié)果滴鼻組小鼠BALF中白細胞總數(shù)最高,其次為霧化組小鼠;且滴鼻組小鼠嗜酸性粒細胞總數(shù)及中性粒細胞總數(shù)亦明顯高于霧化組與正常組,差異有統(tǒng)計學意義(P0.05)。滴鼻組小鼠BALF中IL-4、IL-13水平明顯高于霧化組與正常組小鼠,差異有統(tǒng)計學意義(P0.05);霧化組小鼠BALF中IL-4、IL-13水平高于正常組小鼠,差異有統(tǒng)計學意義(P0.05)。滴鼻組肺組織炎癥反應(yīng)最明顯,并可見上皮細胞破壞,杯狀細胞增生,支氣管和血管周圍有大量炎癥細胞浸潤,以嗜酸性粒細胞和淋巴細胞為主。霧化組炎癥程度較輕,正常對照組無明顯炎癥改變。結(jié)論霧化吸入和經(jīng)鼻滴入的激發(fā)方式都可以成功制作出支氣管哮喘模型,經(jīng)鼻滴入方式可以引起肺部更加明顯的炎癥浸潤及細胞因子表達,為哮喘研究提供更好的選擇。
[Abstract]:Objective to investigate the effect of intranasal spray and two kinds of excitation on the airway inflammation in mice model of bronchial asthma, to provide experimental and theoretical basis for the study and optimization of animal model of bronchial asthma. Methods ovalbumin (OVA) sensitized BALB/c mice were stimulated respectively by two different methods of intranasal or aerosol replication asthma model observation, bronchoalveolar lavage fluid (BALF) and the total number of cells in the classification of white blood cell count, IL-4, ELISA for the detection of BALF in supernatant of IL-13 content, the routine pathological sections with hematoxylin and eosin (HE) staining was used to observe the pulmonary inflammation. Results the total number of white blood cells in the intranasal group BALF in the highest, followed by inhalation group and mice; in the intranasal group number of eosinophils and neutrophils were higher than that in the aerosol group and normal group, the difference was statistically significant (P0.05). IL-4 nasal drops group in BALF, IL-13 The atomization group and the normal group was significantly higher than that in mice, the difference was statistically significant (P0.05); IL-4 inhalation group in BALF mice, the level of IL-13 was higher than in the normal group, the difference was statistically significant (P0.05). The most obvious drop in lung tissue of nasal inflammation and visible destruction of epithelial cells, goblet cell hyperplasia, around the bronchi and blood vessels there are a lot of inflammatory cells infiltration by eosinophils and lymphocytes. The atomization group inflammation to a lesser extent, the normal control group had no obvious inflammatory changes. Conclusion inhalation and excitation of nasal drops can be managed to create a model of bronchial asthma, nasal drops can be expressed more obvious infiltration and inflammation of the lungs cytokines cause, provide better choice for asthma research.

【作者單位】： 華中科技大學同濟醫(yī)學院附屬同濟醫(yī)院呼吸與危重癥醫(yī)學科;
【基金】：國家自然科學基金青年基金資助項目(No.30900647,30900648)
【分類號】：R562.25

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本文編號：1611987